Current levels of suppression of waterhyacinth in Florida USA by classical biological control agents

Waterhyacinth, Eichhornia crassipes (Mart.) Solms (Pontederiaceae), has been a global target for classical biological control efforts for decades. In Florida, herbicidal application is the primary control method employed, usually without regard for the activities of the three biological control agents introduced intentionally during the 1970s, namely Neochetina eichhorniae Warner, Neochetina bruchi, Hustache (Coleoptera: Curculionidae), and Niphograpta albiguttalis Warren (Lepidoptera: Crambidae). A series of field experiments from 2008 to 2010 was conducted at four Florida sites using an insecticide-check approach to quantify the current levels of suppression provided by these agents. In the field N. albiguttalis was rarely found while more than 99% of all Neochetina sp. adults were N. eichhorniae. Although it was not possible to disentangle the relative impacts of Neochetina sp. adults from larvae on individual plant variables, the larvae played a major role in reducing plant biomass and the number of inflorescences. Plots exposed to unrestricted herbivory contained 58.2% less biomass and produced 97.3% fewer inflorescences at the end of the experiments. Despite these large reductions, herbivory decreased waterhyacinth coverage by only 16.8% and most of this was attributed to a low-nutrient site where coverage was reduced disproportionately. Overall, coverage trended upwards during the course of the experiments and was always close to 100% when the plots were harvested. Although coverage is a somewhat arbitrary metric, especially for floating plants subject to compression and dispersion, it influences the perception of biological control efficacy which, in turn, directly influences herbicide management decisions in Florida. Despite waterhyacinth populations that now produce less than half as much biomass and up to 98% fewer seeds than before the deployment of biological control agents, the overall approach used to achieve maintenance control of the plant in Florida will probably not change unless new biological control agents, such as Megamelus scutellaris Berg (Hemiptera: Delphacidae), can reduce coverage significantly.     

Performance and impact of the biological control agent Xubida infusella (Lepidoptera; Pyralidae) on the target weed Eichhornia crassipes (waterhyacinth) and on a non-target plant, Pontederia cordata (pickerelweed) in two nutrient regimes

Xubida infusella (Walker) (Lepidoptera: Pyralidae) is potentially a useful biological control agent targeting Eichhornia crassipes (waterhyacinth) in the USA but many regions infested with waterhyacinth are also inhabited by an alternative native host, Pontederia cordata (pickerelweed). Experiments were conducted in Australia to assess the impact of X. infusella on pickerelweed compared to waterhyacinth where both these plants were available and X. infusella had already been released. Overall X. infusella had a greater impact on pickerelweed than on waterhyacinth. More than one larva per plant was required to reduce the total shoot dry weight of waterhyacinth but only one larva per plant reduced the total shoot dry weight of pickerelweed. Insect feeding caused the number of secondary shoots (daughter plants) of pickerelweed to double whereas the number of daughter plants produced by waterhyacinth remained unchanged. We suggest this indicates a considerable impact on pickerelweed rather than effective compensation for insect damage because the shoots produced were very small. Waterhyacinth produced a constant number of daughter plants when fed on by up to three larvae per plant. Higher nitrogen status of both species of host plant increased the rate of larval development and pupal weight of X. infusella. The weight and fecundity of X. infusella reared on pickerelweed were lower than those reared on waterhyacinth but large numbers of progeny were produced on both plant species. This experiment demonstrates a considerable impact of X. infusella on pickerelweed suggesting this plant is at risk from this agent if released in the USA where pickerelweed is present. The considerable impact on waterhyacinth demonstrates the potential for this insect to contribute to waterhyacinth control in countries where risk assessment favours release.     

凤眼莲-根际微生物系统的降酚效应

 在自然水体中,由于风眼莲的存在,其根际的异养细菌的数量和种类均大量增加,从而可以提高降解水体中的有机物的能力;在实验室条件下,人工组建成风眼莲—根际细菌系统,系统的降酚效应要大于单独的细菌和风眼莲,证实这是一个具高效降酚能力的共生生物系统。本文从野外原位调查和实验室模拟试验两方面,讨论了凤眼莲—根际细菌共生系统的降酚效应,以及其中的机制和应用。     

Mycobiota associated with larval mines of Thrypticus truncatus and T. sagittatus (Diptera, Dolichopodidae) on waterhyacinth, Eichhornia crassipes, in Argentina

Thrypticus truncatus is a candidate agent for biocontrol of waterhyacinth; the larvae of this diptera mine in the petioles and feed on the phloem in the vascular bundles. The mycobiota associated with T. truncatus and T. sagittatus mines was investigated during two surveys undertaken in the spring and autumn in the Delta of the Paraná River, Argentina. Isolations were made from the mines and larval feeding points, as well as from the larvae, following dissection of the petioles, and plated onto agar. Young and upper parts of the petioles without Thrypticus mines were used as controls. Twenty eight fungal species were isolated from the mines. Pestalotiopsis guepinii, Mucor attenuatus, Phoma tropica, Achlya americana, Fusarium avenaceum, Cladosporium cladosporioides, Clonostachys rosea, Epicoccum purpurascens, Plectosporium tabacinum, Alternaria alternata, and Acremonium sp. were the most common fungi associated with mines and feeding points. Cladosporium cladosporioides, Cytospora sp., Mucor attenuatus, and Phoma tropica were associated with the larval body. The list of fungi in mines was compared with bibliographic information to determine if the species are known pathogens on waterhyacinth or other plant species. This is the first study on mycobiota associated with T. truncatus and T. sagittatus mines in waterhyacinth petioles in Argentina.