全文获取类型
收费全文 | 70篇 |
免费 | 0篇 |
国内免费 | 8篇 |
出版年
2021年 | 1篇 |
2015年 | 1篇 |
2014年 | 1篇 |
2012年 | 1篇 |
2011年 | 3篇 |
2010年 | 1篇 |
2009年 | 3篇 |
2008年 | 7篇 |
2007年 | 6篇 |
2006年 | 2篇 |
2005年 | 5篇 |
2004年 | 2篇 |
2003年 | 1篇 |
2001年 | 3篇 |
2000年 | 3篇 |
1999年 | 5篇 |
1997年 | 4篇 |
1996年 | 4篇 |
1995年 | 5篇 |
1994年 | 5篇 |
1993年 | 2篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1990年 | 2篇 |
1989年 | 2篇 |
1988年 | 1篇 |
1987年 | 1篇 |
1986年 | 1篇 |
1985年 | 1篇 |
1983年 | 1篇 |
1980年 | 1篇 |
1979年 | 1篇 |
排序方式: 共有78条查询结果,搜索用时 202 毫秒
1.
胚乳性状的遗传模型和世代平均数 总被引:25,自引:7,他引:18
谷类作物的胚乳是三倍体组织,胚乳性状受3N遗传控制。本文分析了胚乳性状的遗传特征,建立了相应的遗传模型,推导了世代群体的平均数分量,并提出了研究胚乳性状基因效应的一些简单的交配设计。 相似文献
2.
以异源四倍体体细胞杂种为父本杂交培育三倍体柑桔植株的研究 总被引:23,自引:1,他引:22
以 3个柑桔原生质体融合而来的四倍体体细胞杂种为父本 ,与二倍体单胚性种柚子 (Citrusgrandis)以及单多胚混合型品种“华农本地早”桔 (C.reticulata)有性杂交 ,授粉后 90 d,发现种子干瘪 ,大部分种子的胚败育。将干瘪种子在 MT附加 1mg/L GA3 或 50 0 mg/L麦芽浸出物的培养基中 ,经培养抢救 ,有 2 5.6%的种子萌发成苗或继续进行胚的生长 ,后者进一步诱导能形成丛芽 ,经试管嫁接或诱导生根形成完整植株。共获得 6个组合 73棵完整植株 ,染色体数检查表明 ,2 0株为三倍体 (2 n=3x=2 7) ,32株为二倍体 (2 n=2 x=18) ,8株为非整倍体 ,其它 13株还有待于进一步检查。 相似文献
3.
Induced single fertilization in maize 总被引:1,自引:1,他引:0
Akio Kato 《Sexual plant reproduction》1997,10(2):96-100
Bicellular pollen with one vegetative nucleus and one diploid arrested generative cell (”monospermic” pollen) was induced
by trifluralin treatment of diploid maize plants at 7–9 days before flowering. The arrested generative cell (seemingly a diploid
sperm cell) fused with the central cell of diploid plants and produced shriveled endosperm resembling that of a 2n×4n cross
in maize. Dual pollination experiments with a purple embryo marker revealed single fertilization events in which the union
of one sperm cell with the egg occurs but there is no union of a second sperm cell with the central cell. Singly fertilized
ovules survived at least 4 days. Furthermore, many viable triploid plants were obtained. This technique therefore appears
to have the potential for manipulating ploidy level in crops and may become useful in investigating fertilization mechanisms
of angiosperms.
Received: 1 October 1996 / Revision accepted: 8 January 1997 相似文献
4.
Xylooligosaccharides (XOS) with DP 2-4 are important synbiotics used as food ingredients based on its prebiotic characteristics. In this work, the production of XOS from lignocellulosic material was performed by combined chemical-enzymatic methods. Xylan was prepared from triploid Populas tomentosa, and bioconverted into XOS by crude xylanase solution obtained from Pichia stipitis. The effects of reaction time, temperature, enzyme dosage, and pH value on the production of XOS were fully evaluated. Under the optimal condition (25 U g−1 substrate, pH 5.4 and 50 °C), 36.8% of the xylan preparation was converted to XOS, equivalent to 3.95 mg/mL of the hydrolyzate. Xylobiose, xylotriose and xylotetrose were analyzed to be the main products of the enzymatic hydrolyzate, which together accounted for over 95% of the released oligosaccharides. Meanwhile, the effect of sonication pretreatment on the conversion efficiency of the xylan preparation was also investigated. 相似文献
5.
Parthenocarpic fruit set in triploid watermelon induced by CPPUand 2,4-D applications 总被引:1,自引:0,他引:1
J.V.?Maroto A.?Miguel S.?Lopez-GalarzaEmail author A.?San Bautista B.?Pascual J.?Alagarda J.L.?Guardiola 《Plant Growth Regulation》2005,45(3):209-213
The localized application of the synthetic cytokinin CPPU ((2-chloro-4-pyridyl)-N-phenyl urea) to ovaries at flower opening was as effective as free pollination in setting parthenocarpic fruit in the triploid watermelon cultivar ‘Reina de Corazones’, and increased yield per unit land area by at least 50%, simply due to the lack of requirement for diploid pollen producing plants within the orchard. The application of the synthetic auxin 2,4-D (2,4-dichlorophenoxy acetic acid) as a full coverage spray, was also effective in setting fruit; total yield was however 10% smaller than in the CPPU-treated plots, but the cost of application was much less expensive. These applications had no adverse effect on fruit quality, and their effectiveness in commercial watermelon production was evaluated over 4 years. Localized applications of 2,4-D to ovaries were less effective in setting fruit, and increased hollow fruit. 相似文献
6.
Calamus (Acorus calamus L., Araceae) is an aromatic herb, indigenous to Central Asia and Eastern Europe. The fragrant oils obtained by alcoholic extraction of the rhizome are mainly used in the pharmaceutical and oenological industries. Nevertheless, the occurrence of beta-asarone [(Z)-1,2,4-trimethoxy-5-prop-1-enyl-benzene] limits the possibility of its use due to the carcinogenic properties of this compound. The aim of this work was to identify a diploid beta-asarone-free A. calamus by using chemical and molecular approaches. For these purposes alcoholic extracts of both diploid and triploid A. calamus were analyzed by gas chromatography-mass spectrometry (GC-MS) and comparison of the 700 bp sequence of the non-transcribed spacer (NTS) in the 5S-rRNA gene was also performed. Alcoholic extracts of the triploid A. calamus were characterized by a higher percentage of beta-asarone (11%), which was the main compound, followed by higher percentages of camphene (2.27%), E-beta-ocimene (3.28%), camphor (1.54%), calarene (1.42%), alpha-selinene (5.02%) and tau-cadinol (2.00%), when compared to the diploid A. calamus. The latter had higher percentages of iso-shyobunone (8.62%), beta-sesquiphellandrene (3.28%), preiso calamendiol (22.81%) and acorone (26.33%), and completely lacked of beta-asarone. The 5S-rRNA spacer region of both diploid and triploid A. calamus were amplified by PCR using a pair of primers located at the 3' and 5' ends of the coding sequence of 5S-rRNA gene. The resulting PCR products (about 700 bp) were gel purified, subcloned into pGEM-T Easy vector and sequenced. By aligning the isolated nucleotide sequences of the two varieties and the sequences from different A. calamus chemotypes present in Genbank, sequence diversities were found in the spacer region. Furthermore, the PCR products were digested by using EcoRI. The restriction profile of the spacer domain resulted different for the two cytotypes. Along with chemical analysis of alcoholic extracts, sequence analysis coupled to restriction mapping was demonstrated to represent a powerful tool to distinguish the A. calamus diploid cytotype from the others. The security and effective usage of the diploid beta-asarone-free A. calamus was also discussed. 相似文献
7.
海水经济贝类在海水养殖中占有重要的地位,开展贝类育种、育苗对提高重要经济贝类的产量和质量意义重大。多倍体育种作为贝类育种的方法之一,研究者希望通过这种方法,诱导出个体大、生长快、肉质好的三倍体贝类,以提高群体生长速度,改善经济性状,增强养殖生物的抗逆性。对多倍体贝类的亲贝培育、育种原理、诱导方法、检测技术,以及生物学性状作了综述,最后探讨了多倍体研究中存在的主要问题。 相似文献
8.
Microsatellite-centromere distances and microsatellite diversity in different ploidy classes of Chinese shrimp (Fenneropenaeus Chinensis) 总被引:1,自引:0,他引:1
This is the first report of microsatellite-centromere mapping in this commercial species Fenneropenaeus Chinensis, and will be important for providing fixed points in the linkage groups of genetic maps. Triploid Chinese shrimp was induced
by heat shock. The fertilized eggs were treated either by retention of the first polar body or the second polar body to produce
Meiosis I (MI) or Meiosis II (MII) triploid. The triploidy status in each Chinese shrimp could be confirmed by nine polymorphic
microsatellite loci, in which the parents with different alleles and the female parents were each heterozygous. The nine loci
were mapped in relation to their centromeres in three MII triploid families, which were induced by retention of the second
polar bodies after fertilization with sperm. Microsatellite-centromere (M-C) distances ranged from 9.6 cM to 37 cM under the
assumption of complete interference. Information on the positions of centromeres in relation to the microsatellite loci will
represent a contribution towards assembly of genetic maps in F. chinensis. Twelve polymorphic microsatellites were used to assess the heterozygosity and allelic diversity in different ploidy classes.
As expected, triploids were significantly more polymorphic than diploids. The diploids had an average heterozygosity and allelic
diversity value of 0.86, whereas the triploids heterozygosity averaged 0.93 and had allelic diversity value of 1.29. However,
MI triploids were not significantly more polymorphic than MII in the microsatellite loci. 相似文献
9.
A mixture model approach to the mapping of QTL controlling endosperm traits with bulked samples 总被引:1,自引:0,他引:1
Endosperm traits are of triploid inheritance and have become a focus of breeding effort for their close relations with the
grain quality. Current methods for mapping quantitative trait loci (QTL) underlying endosperm traits are restricted to the
use of the phenotypes of single grain samples as input data set, which are often not available in practice due to the small
size of the cereal seeds. This paper proposed a statistical model for one specially tailored mapping strategy, where the marker
genotypes are obtained from the maternal plants in the segregation population and the phenotypic responses are replaced by
the trait means of composite endosperm samples pooled from each plant. It should therefore be more practical and have wide
applicability in mapping endosperm traits. The method was implemented by fitting the phenotypic means of endosperms into a
Gaussian mixture model. Both the exact and approximate Expectation-Maximization algorithms were proposed to estimate the model
parameters. The presence of the QTL was determined by likelihood ratio test statistics. Statistical power and other properties
of the new method were investigated and compared to the current single-seed method under a variety of scenarios through simulation
studies. The simulations suggest a reasonable sample size should be used to ensure reliable results. The proposed method was
also applied to a simulated genome data for further evaluation. As an illustration, a real data of maize was analyzed to find
the loci responsible for the popping expansion volume. 相似文献
10.