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1.
Formation of a Tree having a Low Lignin Content 总被引:2,自引:0,他引:2
Received 30 September 2001/ Accepted in revised form 26 October 2001 相似文献
2.
Jerry W. McClure 《Phytochemistry》1974,13(7):1065-1069
Five-day-old etiolated barley shoots respond to brief illumination with red light by increasing their level of PAL ca 50% within 5 hr. When assayed s 相似文献
3.
The non‐toxic compound N‐acetyl‐L‐phosphinothricin (N‐Ac‐L‐PPT) is used in a so‐called deacetylation system to induce male sterility in transgenic plants by tapetum specific deacetylation to the herbicide L‐phosphinothricin (L‐PPT). A procedure was developed to produce pure racemic and L‐isomeric N‐Ac‐PPT containing less than 30 ppm residual PPT. Experiments applied to wild type tobacco and PPT‐resistant tobacco showed that the maximal tolerated N‐Ac‐PPT concentration would be less than 45 mM of the L‐isomer. Otherwise unspecific deacetylation by several acylases, as well as by environmental conditions like higher temperatures or pHs beyond neutrality, increased the residual L‐PPT content to toxic concentrations. In contrast, N‐acetyl‐L‐phosphinothricyl‐alanyl‐alanine (N‐Ac‐L‐PPTT), a substance also occurring during the biosynthesis of phosphinothricyl‐alanyl‐alanine (PPTT) by some Streptomyces species, was tolerated up to 274 mM by wild type tobacco plants. However, the ArgE deacatylase from Escherichia coli originally used in the deacetylation system, as well as some other acylases, showed no activity towards N‐Ac‐L‐PPTT. 相似文献
4.
《Bioscience, biotechnology, and biochemistry》2013,77(12):2762-2765
Comparative two-dimensional electrophoresis showed six proteins, which were significantly produced in the root of salt-tolerant barley. These proteins were identified as stress/defense-related proteins that do not scavenge reactive oxygen species directly, suggesting that salt-tolerant barley develops not only an antioxidative system, but also physical and biochemical changes to cope with salt stress. 相似文献
5.
Harry J. Klee Yvonne M. Muskopf Charles S. Gasser 《Molecular & general genetics : MGG》1987,210(3):437-442
Summary 5-enolpyruvylshikimate-3-phosphate synthase (EPSPs), the target of the herbicide glyphosate, catalyzes an essential step in the shikimate pathway common to aromatic amino acid biosynthesis. We have cloned an EPSP synthase gene from Arabidopsis thaliana by hybridization with a petunia cDNA probe. The Arabidopsis gene is highly homologous to the petunia gene within the mature enzyme but is only 23% homologous in the chloroplast transit peptide portion. The Arabidopsis gene contains seven introns in exactly the same positions as those in the petunia gene. The introns are, however, significantly smaller in the Arabidopsis gene. This reduction accounts for the significantly smaller size of the gene as compared to the petunia gene. We have fused the gene to the cauliflower mosaic virus 35 S promoter and reintroduced the chimeric gene into Arabidopsis. The resultant overproduction of EPSPs leads to glyphosate tolerance in transformed callus and plants. 相似文献
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Cardarelli M Mariotti D Pomponi M Spanò L Capone I Costantino P 《Molecular & general genetics : MGG》1987,209(3):475-480
Summary Segments of the TL-DNA of the agropine type Ri plasmid pRi 1855 encompassing single and groups of open-reading frames were cloned in the Ti plasmid-derived binary vector system Bin 19. Leaf disc infections on Nicotiana tabacum led to transformed plants, some of which showed typical hairy root phenotypes, such as the wrinkled leaf morphology, excessive and partially non geotropic root systems and the ability of leaf explants to differentiate roots in a hormone-free culture medium. Particularly interestingly, most of these traits were shown by plants transformed with a TL-DNA segment encompassing the single ORF 11, corresponding to the rolB locus. Hairy root can be induced by this latter T-DNA segment on wounded stems of tobacco plants; hairy root induction on carrot discs requires, on the contrary, a more complex complement of TL-DNA genes.Abbreviations YMB
yeast mannitol broth
- MS
Murashige and Skoog medium
- 6-BAP
6-benzylaminopurine
- NAA
naphthalene acetic acid
- Km
kanamycin
- Cb
carbenicillin 相似文献
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10.
Emergence and growth of barley was severely decreased by short periods (less than 24 hours) of pre-emergence waterlogging
at 20°C. The extent of damage depended on a combination of duration of waterlogging, soil water potential and aggregate size.
Potentials of less than—4kPa prevented loss of plants developing in aggregates of less than 2 mm diameter after a transitory
period of waterlogging although some shoot and root damage occurred. By comparison seeds growing in soil consisting of aggregates
greater than 2 mm in diameter were not damaged by transitory waterlogging even when drainage only occurred at−0.8kPa. The
severity of damage increased with the period of waterlogging. A criterion obtained as the product of mean size grade and water
potential gave a single value (−4NM−1) below which emergence was satisfactory. Waterlogging halfway through germination gave more severe damage than near sowing
date or near emergence. 相似文献