中缅树鼩形态性状的遗传制约性初步研究

树鼩是一种在医学和生物学上用途广泛的实验动物遗传资源。主要以上海市肿瘤研究所购自云南昆明地区的35只中缅树鼩及其实验室条件下繁殖的G1代为试验素材,对涉及形态性状遗传制约性的一些指标进行分析, 应用Rife-Buranamanas 定律分析中缅树鼩涉及毛色等诸多具有遗传制约性的外貌特征的显隐性关系,得出中缅树鼩典型的外貌特征为野生型毛色有季节性红斑、白腹、没有白眼圈、掌底肤色为肉色、耳朵不竖、圆形的尾尖、两侧乳房每对之间的连线分别与体轴线垂直。 Abstract : Tree shrew is a kind of excellent experimental animal resource in medical science and biology . In this paper, 35 Tupaia blangeri chinensises (TBCs) captured from Kunming,Yunnan province were investigated. We analyzed hereditary conditionality index about some morphological characters. According to Rife-Buranamanas law, We analyzed the appearance characteristics with hereditary conditionality including color of fur, orbit etc.. The results showed the following: wild fur with seasonal red spot, white fur of abdomen, non-white orbit, fleshcolor palm, non-cocked ear, round tip tail, and the line between the breasts of both sides in a vertical position with axis line.     

Role of cofactors B (TBCB) and E (TBCE) in tubulin heterodimer dissociation

Tubulin folding cofactors B (TBCB) and E (TBCE) are alpha-tubulin binding proteins that, together with Arl2 and cofactors D (TBCD), A (TBCA or p14) and C (TBCC), participate in tubulin biogenesis. TBCD and TBCE have also been implicated in microtubule dynamics through regulation of tubulin heterodimer dissociation. Understanding the in vivo function of these proteins will shed light on the Kenny-Caffey/Sanjad-Sakati syndrome, an important human disorder associated with TBCE. Here we show that, when overexpressed, TBCB depolymerizes microtubules. We found that this function is based on the ability of TBCB to form a binary complex with TBCE that greatly enhances the efficiency of this cofactor to dissociate tubulin in vivo and in vitro. We also show that TBCE, TBCB and alpha-tubulin form a ternary complex after heterodimer dissociation, whereas the free beta-tubulin subunit is recovered by TBCA. These complexes might serve to escort alpha-tubulin towards degradation or recycling, depending on the cell requirements.