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In vitro versus in vivo genetic divergence in potato 总被引:1,自引:0,他引:1
J. Gopal 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1999,98(2):299-304
The objective of this study was to compare the genetic divergence pattern in potato (Solanum tuberosum L.) under in vitro and in vivo conditions. Twenty two potato genotypes were evaluated for ten morphological characters under
four in vitro conditions, and for 20 characters under four in vivo seasons. Mahalanobis’ generalized intra- and inter-group
genetic distances, and the distribution of genotypes into different clusters, led to the same conclusions under both in vitro
and in vivo conditions: (1) genetic diversity was not related to geographic diversity, (2) genetic distances were higher between
Tuberosum and Andigena than within Tuberosum and Andigena, and (3) present-day Indian varieties have more resemblance to Tuberosum
than to the Andigena group. The in vitro approach was more effective than the in vivo approach for differentiating the genotypes
per se, although its effectiveness for cross prediction is known to be low.
Received: 15 September 1997 / Accepted: 15 July 1998 相似文献
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Baker C. Jacyn Mock Norton M. Deahl Kenneth Domek John 《Plant Cell, Tissue and Organ Culture》1997,51(2):111-117
A method is described that allows the rate of oxygen consumption to be monitored in plant cell suspensions. The method utilized
oxygen electrodes placed in beakers of plant cells subjected to various treatments. The voltage readings from calibrated electrodes
were converted to % oxygen (100% equals air equilibration) and the rate of oxygen consumption was estimated by calibration
graphs made with no cells present. This system simultaneously monitors one to sixteen or more samples, allowing comparison
of treatments on identically treated cells. We have used this method to study the respiratory burst of plant cells produced
in response to viable or heat-killed bacteria. Because the system was computer-monitored and open to the atmosphere, data
could be collected over several hours. Various factors that affected the measurement of dissolved oxygen concentration with
this technique were explored and considered.
This revised version was published online in June 2006 with corrections to the Cover Date. 相似文献
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Leah Tsror 《Journal of Phytopathology》2010,158(10):649-658
Black scurf and stem canker on potato is an economically important disease complex, causing both quantitative and qualitative damage to potato crops which occurs in potato production areas throughout the world. The ribosomal DNA internal transcribed spacer sequence analysis is currently accepted and a commonly used method for classifying Rhizoctonia species and anastomosis groups (AGs). To date, 13 AGs have been recognized. The updated AG distribution in potato worldwide production areas confirm the status of AG‐3 as the most prevalent AG in potato and reflects the population dynamics of the pathogen probably due to global trading of tubers. As R. solani is a tuber‐ and soilborne pathogen, the ability to detect its levels in the seed tubers and in the soil and predict the potential damage is an important factor in controlling the disease. Effective disease management of Rhizoctonia disease requires implementation of an integrated disease management approach and knowledge of each of its stages. Although the most important control measures are cultural, chemical control (either by seed tuber‐ or in‐furrow treatments) is still an important tool in reducing the damages caused by R. solani. 相似文献
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Potato tubers ( Solanum tubersum L. cv. Grata) were stored for atleast 1 week at room temperature and then incubated with an equal amount of apples ( Malus domestica L.) for 2 days. After this treatment, intact tuber mitochondria isolated by Percoll gradient centrifugation showed a high degree of induction of the alternative oxidase, measured as cyanide-resistant, salicylhydroxamic acid-sensitive respiration. With succinate as substrate an activity of more than 130 nmol O2 (mg protein) 1 min t was obtained. An assay of the alternative oxidase using duroquinol as an electron donor was developed. To become reliable the assay required the presence of defatted bovine serum albumin (BSA) and catalase (EC 1. 11. 1. 6). Furthermore, a lowering of the assay temperature to 15°C improved the stability of the duroquinol-based activity. One remarkable finding was that with duroquinol (or external NADH) as substrate the alternative oxidase was synergistically activated by succinate (as well as by malate) even in the presence of the succinate dehydrogenase inhibitor malonate. Our interpretation is that succinate and malate (indirectly) activate the alternative oxidase and that this activation is part of a physiological mechanism for regulation of the alternative oxidase. 相似文献
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