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1.
Nitrogen fertigation of greenhouse-grown cucumber 总被引:2,自引:0,他引:2
I. Papadopoulos 《Plant and Soil》1986,93(1):87-93
Summary This greenhouse study investigated the response of trickle-irrigated cucumber (Cucumis sativa cv. ‘Petita’) to three N levels applied with every irrigation via the irrigation stream. The plants were grown in pots filled
with 12 kg of soil. Water containing 5.8, 11.8, or 17.8 mmol N/l, and uniformly supplied with 2.0 and 3.9 mmol/l of P and
K, respectively, was applied two to three times daily. In all treatments of 0.3 leaching fraction was allowed.
The resulting total N applications were 15.7, 31., and 47.2 g N/plant. The total amount of water applied was 1851/plant. Total
N and NO3-N, in lajinae and petioles, increased with increasing N level whereas P and K in generated decreased. Although different
NO3/NH4 ratios in the treatments may have influeced the response to N, it could be concluded that the highest yield was obtained
with 11.8 mmol N/1 due to increased number of fruit. In the root volume of this treatment the NO3-N concentration in the soil solution was aroun 7 mmol/1 for most of the growing season. The dry matter concentration of fruits
was not affected by the N levels.
It was concluded that 11.8 mmol N/1 applied with every irrigation via the irrigation stream is adequate to cover the needs
of greenhous-grown cucumber for higher yield (9.42 kg/plant over a harvesting period of 93 days). 相似文献
2.
We present a single-step procedure for the specific mass labeling of unblocked protein N termini. We show that the dye fluorescamine, which is commonly assumed to require mildly alkaline conditions for undergoing a nonspecific reaction with α- and ε-amino groups associated with amino acids, in fact shows a specific reaction only with α-amino groups present at protein N termini when mildly acidic conditions are used. We use this finding to label, identify, and sequence the trypsinolysis-derived N-terminal peptide of lysozyme, using only mass spectrometry, to illustrate how this method could be used with other proteins. 相似文献
3.
4.
Several unit-length minicircles from the kinetoplast DNA of Leishmania tarentolae were cloned into pBR322 and into M13 phage vectors. The complete nucleotide sequences of three different partially homologous minicircles were obtained. The molecules contained a region of approx. 80% sequence homology extending for 160–270 bp and a region unique to each minicircle. A 14-mer was found to be conserved in all kinetoplast minicircle sequences reported to date. The frequency distributions of various minicircle sequence classes in L. tarentolae were obtained by quantitative gel electrophoresis and by examination of the “T ladder” patterns of minicircles randomly cloned into M13 at several sites. By these methods we could assign approx. 50% of the total minicircle DNA into a minimum of five sequence classes. A sequence-dependent polyacrylamide gel migration abnormality was observed with several minicircle fragments both cloned and uncloned. The abnormality was dependent on the presence of a portion of the conserved region of the minicircle. 相似文献
5.
Summary Amylase, dehydrogenase, arylsulphatase and phosphatases activities were measured in a clay-loam soil amended with seven different
crop residues. All enzyme activities, except phosphomonoesterase, were generally higher in the derived soil samples than in
the original soil. Addition of tobacco and sunflower residues caused an increase on most of the enzyme activities while tomato
residues increased only the amylase and phosphodiesterase activities.
As the enzyme activities were positively correlated to each other, a common source of the enzymes is suggested even though
the coefficients of correlation demonstrate that only a low percentage of the variability can be ascribed to the interactions
among enzyme activities. 相似文献
6.
Enumeration of denitrifying microbial populations in turf 总被引:2,自引:0,他引:2
Summary Denitrifer populations of a silt and silt loam soil under a Kentucky bluegrass turf were enumerated using the most probable
number (MPN) procedure. The influence of soil texture, soil depth, soil moisture, and additions of nitrate fertilizer on denitrifier
populations were determined.
Saturated soil conditions increased denitrifier populations 87-fold in the silt soil and 121-fold in the silt loam soil. Denitrifier
populations did not differ significantly between soil depths and additions of fertilizer nitrate did not influence populations. 相似文献
7.
Gamze Gülez 《Journal of microbiological methods》2010,82(3):324-326
To study bacterial behavior under varying hydration conditions similar to surface soil, we have developed a system called the Pressurized Porous Surface Model (PPSM). Thin liquid films created by imposing a matric potential of − 0.4 MPa impact gene expression and colony development in Pseudomonas putida. 相似文献
8.
This is the first report on using green fluorescent protein (GFP) as a pH reporter in plants. Proton fluxes and pH regulation play important roles in plant cellular activity and therefore, it would be extremely helpful to have a plant gene reporter system for rapid, non‐invasive visualization of intracellular pH changes. In order to develop such a system, we constructed three vectors for transient and stable transformation of plant cells with a pH‐sensitive derivative of green fluorescent protein. Using these vectors, transgenic Arabidopsis thaliana and tobacco plants were produced. Here the application of pH‐sensitive GFP technology in plants is described and, for the first time, the visualization of pH gradients between different developmental compartments in intact whole‐root tissues of A. thaliana is reported. The utility of pH‐sensitive GFP in revealing rapid, environmentally induced changes in cytoplasmic pH in roots is also demonstrated. 相似文献
9.
Influence of ionic strength and pH on the interaction between high-affinity heparin and antithrombin
Birgitta Nordenman Ingemar Björk 《Biochimica et Biophysica Acta (BBA)/General Subjects》1981,672(3):227-238
Binding constants for the binding of high-affinity heparin to antithrombin at different ionic strengths were determined by fluorescence titrations and were also estimated from dissociation curves of the heparin-antithrombin complex. These curves were monitored by near-ultraviolet circular dichroism or fluorescence. The dependence of the binding constant on the activity of NaCl suggested that maximally 5–6 charged groups are directly involved in the interaction between the two macromolecules. Major pH-dependent changes of the interaction, as evident by changes of the spectroscopic properties of the complex between the molecules, were found to occur below pH 5.5 and above pH 8.5. The acid change, which was irreversible, was most likely caused by an irreversible conformational change of antithrombin. At alkaline pH, however, the gross conformation of antithrombin was stable up to pH 12, while the affinity of high-affinity heparin for antithrombin began to decrease markedly at pH 8.5. The dissociation curve, which was reversible, had a midpoint around pH 9.5. This is compatible with the loss of affinity being caused by either a local conformational change, by ionization of tyrosine or by titration of one or more amino groups. 相似文献
10.
《Cell calcium》2019
Alterations in the (bio)chemical and physical microenvironment of cells accompany and often promote disease formation and progression. This is particularly well established for solid cancers, which are typically stiffer than the healthy tissue in which they arise, and often display profound acidification of their interstitial fluid. Cell surface receptors can sense changes in the mechanical and (bio)chemical properties of the surrounding extracellular matrix and fluid, and signalling through these receptors is thought to play a key role in disease development and advancement. This review will look at ion channels and G protein coupled receptors that are activated by mechanical cues and extracellular acidosis, and stimulation of which results in increases in intracellular Ca2+ concentrations. Cellular Ca2+ levels are dysregulated in cancer as well as cancer-associated cells, and mechano- and proton-sensing proteins likely contribute to these aberrant intracellular Ca2+ signals, making them attractive targets for therapeutic intervention. 相似文献