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Protein preparations from seeds and seedlings (cotyledons) of rape (Brassica napus subsp. napus [L.] DC.) catalyzed the transfer of sinapic acid from 1-Osinapoyl--glucose to malate in the formation of O-s-inapoylmalate. The enzyme involved, 1-O-sinapoyl--glucose: l-malate O-sinapoyltransferase (SMT; EC 2.3.1), catalyzes the key step in the overall conversion of the seed constituent sinapine (O-sinapoylcholine) to the accumulating O-sinapoylmalate by way of the intermediate 1-O-sinapoyl--glucose. The present paper describes this phenomenon focussing on SMT activity.Abbreviations Sin-Glc
1-O-sinapoyl--glucose
- Sin-Mal
O-sinapoylmalate
- SMT
1-O-sinapoyl--glucose: l-malate sinapoyltransferase (EC 2.3.1)
This work was supported by the Deutsche Forschungsgemeinschaft, the Fonds der Chemischen Industrie and the Ontario Ministry of Agriculture and Food. 相似文献
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