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Effect of interleukin-8 on production of tumor-associated substances and autocrine growth of human liver and pancreatic cancer cells 总被引:12,自引:0,他引:12
Megumi Miyamoto Yukihiro Shimizu Kazuhiko Okada Yoshiro Kashii Kiyohiro Higuchi Akiharu Watanabe 《Cancer immunology, immunotherapy : CII》1998,47(1):47-57
We have previously reported that human liver cancer cell lines produce interleukin-8 (IL-8) at high levels. Those tumor cells
appeared to express two kinds of IL-8 receptor on their surface. In order to analyze the role of IL-8 on the biological characteristics
of those tumor cells, we suppressed IL-8 production from human liver (HuH-7 and HuCC-T1) and pancreatic cancer cell lines
(HuP-T4) by treatment with IL-8 antisense oligonucleotides. Suppression of IL-8 production resulted not only in inhibition
of cell growth, but also in an increase in the concentrations of some tumor-associated substances such as carbohydrate antigen
19-9 (CA19-9) in the medium. These data indicate that IL-8 produced by human liver and pancreatic tumors may act as an autocrine
growth factor and may control the production of some tumor-associated substances. Furthermore, surface expression of sialyl-Lewisa, which is a ligand for ELAM-1 on human umbilical vein endothelial cells (HUVEC), HuCC-T1 and HuP-T4 cells was decreased and
the attachment of these tumor cells to HUVEC was inhibited by treatment with IL-8 antisense oligonucleotide. Since the soluble
form of CA19-9 (sialyl-Lewisa) was shown to inhibit the tumor cell binding to HUVEC, the decrease in release of CA19-9 into the medium and increase in
the expression of sialyl-Lewisa on the cell surface may suggest that IL-8 production from the tumor cells enhances metastatic potential by augmenting the
binding activity of the tumor cells to HUVEC. These data demonstrate that a cytokine produced by tumor cells may function
as an autocrine growth factor and affect tumor cell dissemination.
Received: 4 February 1998 / Accepted: 6 May 1998 相似文献
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