Numerical abundance of myxomycetes (myxogastrids) in soils in the West of England

Abstract A most-probable-number technique was used to quantify the abundance of myxomycetes (myxogastrids) in soil samples taken from 23 'Sites of Special Scientific Interest' and one other site in the West of England. Associated organisms and soil conditions were also recorded. Sixteen of the 24 sites yielded myxomycete plasmodium-forming units (PFUs) in numbers averaging 230 cm⁻³ fresh soil. Where detectable in samples of grassland soil, the numbers ranged from 20–2750 cm⁻³, in woodland soil from 20–800 cm⁻³, and in sand dunes from 80–400 cm⁻³. Repeated sampling revealed changing numbers at single sites. The abundance of PFUs was correlated positively with numbers of soil amoebae, ciliates and nematodes, and with levels of magnesium and soil density, and negatively with organic matter content and levels of NH₄-nitrogen and phosphate. Each PFU probably corresponded to one or several uninucleate cells in the soil rather than to a plasmodium.     

THE LASER TOMOGRAPHICAL METHOD USINGMINIMUM OF PROJECTION FOR BIOLOGICAL OBJECT STRUCTURE STUDY

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Binding of simple carbohydrates and some N-acetyllactosamine-containing oligosaccharides to Erythrina cristagalli agglutinin as followed with a fluorescent indicator ligand

Erythrina cristagalli agglutinin, a dimeric lectin [J. L. Iglesias, et al. (1982) Eur. J. Biochem.123, 247–252] was shown by equilibrium dialysis to be bivalent for 4-methylumbelliferyl-β-d-galactoside. Upon binding to the lectin, this ligand showed a difference absorption spectrum with two maxima (at 322 and 336 nm) of equal intensity (Δ? = 1.2 × 10³m^?1 cm^?1). A similar spectrum with a comparable value of Δ? was obtained with 4-methylumbelliferyl-N-acetyl-β-d-galactosaminide. Binding of methyl-α-d-galactoside, lactose, and N-acetyllactosamine all produced small but equally intense protein difference spectra with a maximum (Δ? = 2.8 × 10² M^?1 cm^?1) at 291.6 nm. Upon binding of N-dansyl-d-galactosamine to the lectin, there was a fivefold increase in fluorescence intensity of this ligand. The association constant for N-dansyl-d-galactosamine was caused by a very favorable ΔS° of the dansyl group without affecting the strictly carbohydrate-specific character of binding. N-Dansyl-d-galactosamine was employed as a fluorescent indicator ligand in substitution titrations. This involved the use of simple carbohydrates, N-acetyllactosamine, and oligosaccharides which occur in the carbohydrate units of N-glycoproteins; the latter were Gal(β → 4)GlcNAc(β1 → 2)Man, Gal(β1 → 4)GlcNAc(β1 → 6)Man, and Gal(β1 → 4)GlcNAc(β1 → 6)[Gal(β1 → 4)GlcNAc(β1 → 2)]Man. The titrations were performed at two temperatures to determine the thermodynamic parameters. In the series N-acetyl-d-galactosamine, methyl-α-d-galactoside, and lactose, ?ΔH° increased from 24 to 41 kJ mol^?1; it increased further for N-acetyllactosamine and then remained unchanged for the N-acetyllactosamine-containing oligosaccharides (55 ± 1 kJ mol^?1). This indicated that the site specifically accommodated the disaccharide structure with an important contribution of the 2-acetamido group in the penultimate sugar. Beyond this, no additional contacts seemed to be formed. This conclusion also followed from considerations of ΔS° values which became more unfavorable in the above series (?23 to ?101 ± 4 J mol^?1 K^?1); the most negative value of ΔS° was observed with N-acetyllactosamine and the three N-acetyllactosamine-containing oligosaccharides.     

科研设计性大实验在微生物学实验教学中的应用

探讨了在工科专业的微生物实验教学中结合科研工作开展科研设计性大实验的方法与对师生的影响, 分析了开展科研设计性大实验存在的问题。科研设计性大实验在本科生教学实践中的应用表明该方法可促进本科生的专业学习, 提升本科生的理论应用能力及科研创新能力, 是具有推广意义的教学新模式。     

When physics and biology meet: the nanoscale case

As an illustration of the complexities involved in connecting physics and molecular biology at the nanoscale, in this paper I discuss two case studies from nanoscience. The first examines the use of a biological structure (DNA) to build nanostructures in a controlled way. The second discusses the attempt to build a single molecular wire, and then decide whether such a wire is indeed conducting. After presenting the central features of each case study, I examine the role played in them by microscopic imaging, the different styles of reasoning involved, and the various theoretical, methodological, and axiological differences. I conclude by arguing that, except for the probe microscopes that are used, there is very little in common between the two cases. At the nanoscale, physics and molecular biology seem to meet in a non-unified way.     

Play behaviour and active training of Montagu’s harrier (Circus pygargus) offspring in the post-fledging period

Play bouts and active training of juveniles by Montagus harrier (Circus pygargus) adults in the post-fledging period were observed. Fledglings often played with prey and with a variety of inanimate objects such as bits of moss, regurgitated pellets, sticks and a wad of hay. Inanimate objects selected for play were, in length, very similar to the common vole (Microtus arvalis), which is the most common prey of the species during their breeding period. Some recently fledged individuals were trained to capture invertebrate prey by adults demonstrating techniques for the fledglings and thus develop their hunting skills. Training sessions took place only in the foraging areas of the adult birds.     

Wooden objects from Ohalo II (23,000 cal BP), Jordan Valley, Israel

Eight wooden objects were found at Ohalo II, a submerged and well-preserved site in the Sea of Galilee, Israel. The fisher-hunter-gatherers' site has been radiometrically dated to 22,500-23,500 (cal BP) with 45 assays read by four laboratories. The wooden objects were found on brush-hut floors. They include a bark plank with polish and use signs, pencil-shaped specimens with longitudinal shavings, and other types that may have been decorative or symbolic. One incised wooden object is identical in size and incision pattern to a gazelle bone implement found in a grave, behind a human skull. The recovered wooden objects are not directly related to hunting, gathering, or fishing, and frustratingly, there are no remains of bows, arrows, spears, handles, or other such items. Nonetheless, the objects present a wide repertoire in terms of size, shape, and possible function. The new finds add to the growing body of evidence concerning the use of perishable materials during the Upper Paleolithic.     

Children's ideas of animals' internal structures

Children's understanding about animal internal structure can be affected by several factors which are poorly understoodby teachers. We conducted a large sample study (n=702) of children aged 6–16 years (Grades 1-9) examining children's responses to animals of various size, species and dimensions (2D and 3D objects), and exploring factors which might affect the development of their knowledge. Each child made a drawing of what they thought was inside a specimen animal. We found that using 2D representations of an animal negatively affected the content of children drawings.The effect of animalsize was shown only in children's understanding of skeletons, but not organ systems. The effect of animal species showedclear significant effect of children's ideas on both organ systems and skeleton. Investigation showed that parents' education level, children's experiences with animal-rearing and age significantly affected their scores from organ system. Surprisingly, school books were most frequently cited as sources of children's knowledge rather than their own experience, or information from the internet, encyclopaedias, television or parents.