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Tougou M Furutani N Yamagishi N Shizukawa Y Takahata Y Hidaka S 《Plant cell reports》2006,25(11):1213-1218
In an attempt to generate soybean plants resistant to soybean dwarf virus (SbDV), we transformed a construct containing inverted repeat-SbDV coat protein (CP) genes spaced by β-glucuronidase (GUS) sequences into soybean somatic embryos via microprojectile bombardment. Three T0 plants with an introduced CP gene were obtained, and one generated T1 seeds. The presence of the transgene in T1 plants was confirmed by PCR and Southern blot hybridization analysis, but expression of CP was not detected by northern blot hybridization analysis. Two months after inoculation of SbDV by aphid, T2 plants contained little SbDV-specific RNA and remained symptomless. These plants contained SbDV-CP-specific siRNA. These results suggest that the T2 plants achieved resistance to SbDV by an RNA-silencing-mediated process. 相似文献
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<Emphasis Type="Italic">Soybean dwarf virus</Emphasis>-resistant transgenic soybeans with the sense coat protein gene 总被引:2,自引:1,他引:1
Tougou M Yamagishi N Furutani N Shizukawa Y Takahata Y Hidaka S 《Plant cell reports》2007,26(11):1967-1975
We transformed a construct containing the sense coat protein (CP) gene of Soybean dwarf virus (SbDV) into soybean somatic embryos via microprojectile bombardment to acquire SbDV-resistant soybean plants. Six independent T(0) plants were obtained. One of these transgenic lines was subjected to further extensive analysis. Three different insertion patterns of Southern blot hybridization analysis in T(1) plants suggested that these insertions introduced in T(0) plants were segregated from each other or co-inherited in T(1) progenies. These insertions were classified into two types, which overexpressed SbDV-CP mRNA and accumulated SbDV-CP-specific short interfering RNA (siRNA), or repressed accumulation of SbDV-CP mRNA and siRNA by RNA analysis prior to SbDV inoculation. After inoculation of SbDV by the aphids, most T(2) plants of this transgenic line remained symptomless, contained little SbDV-specific RNA by RNA dot-blot hybridization analysis and exhibited SbDV-CP-specific siRNA. We discuss here the possible mechanisms of the achieved resistance, including the RNA silencing. 相似文献
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Atsushi Uchibori Jun Sasaki Toru Takeuchi Motokazu Kamiya Akiko Tazawa Tsuyoshi Inukai Chikara Masuta 《Molecular breeding : new strategies in plant improvement》2009,23(2):323-328
Soybean dwarf virus (SbDV), a member of the Luteoviridae family, causes serious yield losses in soybean production in northern Japan. We previously found that an Indonesian soybean
cv. Wilis had a high level of resistance to SbDV. Although Wilis is infected by SbDV, symptoms are always mild and develop
considerably later compared with many susceptible cultivars. To identify the resistance gene(s) to SbDV in Wilis in a quantitative
trait loci (QTL) analysis, we used 71 recombinant inbred lines derived from a cross between Wilis and the susceptible Japanese
cv. Toyokomachi and a set of published simple sequence repeat (SSR) markers. The SbDV resistance in Wilis was mainly controlled
by a single QTL located near the SSR marker Sat_271 on the linkage group A1. This QTL accounted for 79% of the phenotypic
variance.
A. Uchibori and J. Sasaki equally contributed to this work. 相似文献
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