排序方式: 共有24条查询结果,搜索用时 31 毫秒
1.
《Bioscience, biotechnology, and biochemistry》2013,77(8):2219-2223
Disruption of an SCS2 ortholog impaired the growth of the alkane-assimilating yeast Yarrowia lipolytica on n-alkanes, particularly on n-decane, although the mRNA level of the ALK1 gene encoding a highly inducible cytochrome P450ALK was not much affected. The same disruption did not cause inositol auxotrophy, implying that Y. lipolytica SCS2 has a different function from its Saccharomyces cerevisiae counterpart. 相似文献
2.
长双歧杆菌TTF菌株增强机体免疫活性研究 总被引:7,自引:0,他引:7
通过长双岐杆菌TTF活菌、菌体破碎物和发酵上清液3种处理物灌胃正常小鼠和免疫功能低下小鼠试验发现,长双歧杆菌TTF的3种受试物对环磷酰胺(Cy)造成的免疫功能低下小鼠模型(IDMM)3项免疫指标均有显著(P〈0.05)或极显著(P〈0.01)影响,且在本试验选取的3个剂量范围内基本呈量效关系。B.longum TTF的3种受试物对IDMM的非特异性免疫功能的影响普遍大于对小鼠的细胞免疫和体液免疫的影响;对正常小鼠免疫功能影响的水平低于其对IDMM各项免疫指标的影响,且无论活菌、菌体破碎物、发酵上清液均不会改变小鼠自身正常的免疫功能。急性毒性试验表明,B.longum,TTF3种处理物对健康小鼠无急性毒性。就3种处理物来讲,发酵上清液组的效果高于菌体破碎物和活菌。 相似文献
3.
Biomedical spectroscopic experiments generate large volumes of data. For accurate, robust diagnostic tools the data must be analyzed for only a few characteristic observations per subject, and a large number of subjects must be studied. We describe here two of the current data analytic approaches applied to this problem: SIMCA (principal component analysis, partial least squares), and the statistical classification strategy (SCS). We demonstrate the application of the SCS by three examples of its use in analyzing 1H NMR spectra: screening for colon cancer, characterization of thyroid cancer, and distinguishing cancer from cholangitis in the biliary tract. 相似文献
4.
Rising global demand for food and population increases are driving the need for improved crop productivity over the next 30 years. Plants have inherent metabolic limitations on productivity such as inefficiencies in carbon fixation and sensitivity to environmental conditions. Bacteria and archaea inhabit some of the most inhospitable environments on the planet and possess unique metabolic pathways and genes to cope with these conditions. Microbial genes involved in carbon fixation, abiotic stress tolerance, and nutrient acquisition have been utilized in plants to enhance plant phenotypes by increasing yield, photosynthesis, and abiotic stress tolerance. Transgenic plants expressing bacterial and archaeal genes will be discussed along with emerging strategies and tools to increase plant growth and yield. 相似文献
5.
6.
7.
Janssen SP Gerard S Raijmakers ME Truin M Van Kleef M Joosten EA 《Neurochemistry international》2012,60(1):21-30
Elevated spinal extracellular γ-aminobutyric acid (GABA) levels have been described during spinal cord stimulation (SCS)-induced analgesia in experimental chronic peripheral neuropathy. Interestingly, these increased GABA levels strongly exceeded the time frame of SCS-induced analgesia. In line with the former, pharmacologically-enhanced extracellular GABA levels by GABAB receptor agonists in combination with SCS in non-responders to SCS solely could convert these non-responders into responders. However, similar treatment with GABAA receptor agonists and SCS is known to be less efficient. Since K+ Cl− cotransporter 2 (KCC2) functionality strongly determines proper GABAA receptor-mediated inhibition, both decreased numbers of GABAA receptors as well as reduced KCC2 protein expression might play a pivotal role in this loss of GABAA receptor-mediated inhibition in non-responders. Here, we explored the mechanisms underlying both changes in extracellular GABA levels and impaired GABAA receptor-mediated inhibition after 30 min of SCS in rats suffering from partial sciatic nerve ligation (PSNL). Immediately after cessation of SCS, a decreased spinal intracellular dorsal horn GABA-immunoreactivity was observed in responders when compared to non-responders or sham SCS rats. One hour later however, GABA-immunoreactivity was already increased to similar levels as those observed in non-responder or sham SCS rats. These changes did not coincide with alterations in the number of GABA-immunoreactive cells. C-Fos/GABA double-fluorescence clearly confirmed a SCS-induced activation of GABA-immunoreactive cells in responders immediately after SCS. Differences in spinal dorsal horn GABAA receptor-immunoreactivity and KCC2 protein levels were absent between all SCS groups. However, KCC2 protein levels were significantly decreased compared to sham PSNL animals. In conclusion, reduced intracellular GABA levels are only present during the time frame of SCS in responders and strongly point to a SCS-mediated on/off GABAergic release mechanism. Furthermore, a KCC2-dependent impaired GABAA receptor-mediated inhibition seems to be present both in responders and non-responders to SCS due to similar KCC2 and GABAA receptor levels. 相似文献
8.
Kanzo Suzuki Fumitaka Kawakami Hisashi Sasaki Hiroko Maruyama Kenzo Ohtsuki 《Biochimica et Biophysica Acta (BBA)/General Subjects》2009
Background
We recently reported that both sulfatide and cholesterol-3-sulfate (SCS) function as potent stimulators for the GSK-3β-mediated phosphorylation of tau protein (TP) in vitro [J. Biochem. 143 (2008) 359–367].Methods
By means of successive gel filtration on a Superdex 200 pg column and three distinct ion-exchange column chromatographies, TP and its associated proteins were highly purified from the extract of rat brain.Results
We found that (i) syndapin 1 and novel protein kinase C? (nPKC?) were identified as the TP-associated proteins; (ii) SCS highly stimulated the phosphorylation of TP and syndapin 1 by nPKC? as well as CK1; (iii) the full phosphorylation of TP and syndapin 1 by nPKC? in the presence of sulfatide resulted in their dissociation; (iv) TP primed by CK1 functioned as an effective phosphate acceptor for GSK-3β; (v) syndapin 1 highly stimulated the GSK-3β-mediated phosphorylation of TP; and (vi) TP isoforms were highly expressed in aged brain, whereas syndapin 1 was consistently detected in adult brain, but not in newborn brain.General significance
These results provided here suggest that (i) TP-associated nPKC? suppresses the GSK-3β-mediated phosphorylation of TP through the phosphorylation of GSK-3β by the kinase in vitro; and (ii) SCS act as effective sole mediators to induce the GSK-3β-mediated high phosphorylation of both TP and its associated syndapin 1 involved in the biochemical processes of neuronal diseases, including Alzheimer's disease. 相似文献9.
The yeast VAMP-associated protein (VAP) homolog Scs2p is an endoplasmic reticulum (ER)/nuclear membrane protein that binds to an FFAT (diphenylalanine in an acidic tract) motif found in various lipid-metabolic proteins, including Opi1p, a negative regulator of phospholipid biosynthesis. Here, we show that Scs2p is a novel phosphoinositide-binding protein that can bind to phosphatidylinositol monophosphates and bisphosphates in vitro. The phosphoinositide-binding domain was assigned to the N-terminal major sperm protein (MSP) domain which also contains the FFAT-binding domain. When several lysine residues in the MSP domain were substituted for alanine, the resulting mutant Scs2 proteins lost the phosphoinositide-binding ability and failed to complement the inositol auxotrophy of an scs2 deletion strain. However, the mutant proteins still localized in the ER/nuclear membrane, in a similar manner to wild-type Scs2p. These results suggest the possibility that Scs2p activity is regulated by phosphoinositides to coordinate phospholipid biosynthesis in response to changes in phospholipid composition. 相似文献
10.