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Celso Sant'Anna Ernesto S. Nakayasu Miria G. Pereira Daniela Lourenço Wanderley de Souza Igor C. Almeida Dr. Narcisa L. Cunha‐e‐Silva 《Proteomics》2009,9(7):1782-1794
Reservosomes are the endpoint of the endocytic pathway in Trypanosoma cruzi epimastigotes. These organelles have the particular ability to concentrate proteins and lipids obtained from medium together with the main proteolytic enzymes originated from the secretory pathway, being at the same time a storage organelle and the main site of protein degradation. Subcellular proteomics have been extensively used for profiling organelles in different cell types. Here, we combine cell fractionation and LC‐MS/MS analysis to identify reservosome‐resident proteins. Starting from a purified reservosome fraction, we established a protocol to isolate reservosome membranes. Transmission electron microscopy was applied to confirm the purity of the fractions. To achieve a better coverage of identified proteins we analyzed the fractions separately and combined the results. LC‐MS/MS analysis identified in total 709 T. cruzi‐specific proteins; of these, 456 had predicted function and 253 were classified as hypothetical proteins. We could confirm the presence of most of the proteins validated by previous work and identify new proteins from different classes such as enzymes, proton pumps, transport proteins, and others. The definition of the reservosome protein profile is a good tool to assess their molecular signature, identify molecular markers, and understand their relationship with different organelles. 相似文献
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Sant'Anna C Parussini F Lourenço D de Souza W Cazzulo JJ Cunha-e-Silva NL 《Histochemistry and cell biology》2008,130(6):1187-1198
Trypanosoma cruzi epimastigote forms concentrate their major protease, cruzipain, in the same compartment where these parasites store macromolecules
obtained from medium and for this ability these organelles were named as reservosomes. Intracellular digestion occurs mainly
inside reservosomes and seems to be modulated by cruzipain and its natural inhibitor chagasin that also concentrates in reservosomes.
T. cruzi mammalian forms, trypomastigotes and amastigotes, are unable to capture macromolecules by endocytosis, but also express cruzipain
and chagasin, whose role in infectivity has been described. In this paper, we demonstrate that trypomastigotes and amastigotes
also concentrate cruzipain, chagasin as well as serine carboxypeptidase in hydrolase-rich compartments of acidic nature. The
presence of P-type proton ATPase indicates that this compartment is acidified by the same enzyme as epimastigote endocytic
compartments. Electron microscopy analyzes showed that these organelles are placed at the posterior region of the parasite
body, are single membrane bound and possess an electron-dense matrix with electronlucent inclusions. Three-dimensional reconstruction
showed that these compartments have different size and shape in trypomastigotes and amastigotes. Based on these evidences,
we suggest that all T. cruzi developmental stages present lysosome-related organelles that in epimastigotes have the additional and unique ability of
storing cargo. 相似文献
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