诱导多能干细胞研究进展

用4个外源基因从完全分化的人成纤维细胞诱导获得了具有胚胎干细胞特性的诱导多能干细胞(iPS细胞),成功逆转了细胞单向发育的规则,取得了细胞重编程和干细胞研究中的重大突破.围绕c-Myc基因和基因载体、诱导效率和外源基因替代因子等方面的研究内容,综述了自2007年人iPS细胞构建至今,国内外在改进iPS细胞诱导方法上的主要研究进展.     

Aldolase A Enhances Intrahepatic Cholangiocarcinoma Proliferation and Invasion through Promoting Glycolysis

Energy metabolism reprogramming has been implicated in tumorigenesis and development. Key metabolism enzyme Aldolase A (ALDOA) has been shown to be highly expressed and involved in various kinds of cancers including hepatocellular carcinoma. In this study, we found that ALDOA was highly expressed in clinical intrahepatic cholangiocarcinoma (ICC) tissues, and its high expression was negatively correlated with overall survival (OS) and recurrence-free survival (RFS) in ICC patients. Knockdown of ALDOA expression significantly inhibited the proliferation and migration of ICC both in vitro and in vivo, while highly-expressed ALDOA in ICC cells promoted the proliferation and migration of ICC cells. By applying ALDOA inhibitor and metabolic mass spectrometry tests, we demonstrated that ALDOA modulated the biological characteristics and metabolic level of ICC cells depending on its enzymatic activity. In summary, ALDOA promotes ICC proliferation and migration by enhancing ICC cells glycolysis. Blocking enzymatic activity of ALDOA provides a strategy to inhibit ICC.