S100 protein-immunoreactive trigeminal neurons innervating the rat molar tooth pulp

S100-immunoreactivity (ir) was examined in tooth pulp primary neurons of the rat. An immunofluorescence method demonstrated that the molar tooth pulp contained S100-immunoreactive (ir) nerve fibers. In the root pulp, pulp horn and roof of the pulp chamber, S100-ir smooth and varicose fibers ramified and formed subodontoblastic nerve plexuses. All the fibers became varicose at the base of the odontoblastic layer and extended to the odontoblastic layer. Some varicose endings could be traced into the dentin. The trigeminal neurons retrogradely labeled with fluorogold (FG) from the first and second maxillary molar tooth pulps exhibited S100- and parvalbumin-ir. Approximately 60% and 24% of the labeled cells were ir for S100 and parvalbumin, respectively. Virtually all parvalbumin-ir FG-labeled cells showed S100-ir, while 40% of S100-ir ones coexpressed parvalbumin-ir. An immunoelectron microscopic method revealed that all myelinated axons and half of the unmyelinated axons in the root pulp contained S100-ir. In the odontoblastic layer, predentin and dentin, S100-ir neurites lost the Schwann cell ensheathment and made close contact with cell bodies and processes of odontoblasts. The odontoblastic layer also contained parvalbumin-ir neurites. These neurites were devoid of the Schwann cell ensheathment and in close apposition to cell bodies and processes of odontoblasts. S100-ir pulpal axons seemed to be insensitive to repeated neonatal capsaicin treatment. This study suggests that S100-ir tooth pulp primary neurons are mostly myelinated and that S100-ir unmyelinated axons in the root pulp are preterminal segments of myelinated stem axons.     

阔筋膜与涤纶布修补术对胸壁肿瘤切除后胸壁缺损的临床疗效

目的:探讨阔筋膜修补术与涤纶布修补术对胸壁肿瘤切除后胸壁缺损的临床疗效观察。方法:选取我院胸外科收治的早期胸壁肿瘤患者81例,随机分为阔筋膜组40例行阔筋膜修补术,涤纶布组41例行涤纶布修补术。比较两组患者术后镇痛药用量、肺部体征、引流量、CRP、白细胞及临床康复情况。结果:治疗后与阔筋膜组比较,涤纶布组患者术后镇痛药量、呼吸受限时间及胸膜粘连发生率均较低,(P0.05)。与治疗前比较,两组患者白细胞水平均升高(P0.05),C反应蛋白水平均降低,(P0.05)。与阔筋膜组相比较,涤纶组白细胞水平,C反应蛋白水平均较低(P0.05),涤纶布组治疗后SPO2水平恢复正常的天数缩短(P0.05)。与阔筋膜组比较,涤纶布组患者术后发热天数,引流天数及住院天数均较少,(P0.05)。结论:涤纶布修补术较传统阔筋膜修补术临床疗效优越,且患者痛苦小,术后恢复快,对临床具有指导意义。     

Structural elucidation of the EPS of slime producing Brevundimonas vesicularis sp. isolated from a paper machine

The slime forming bacteria Brevundimonas vesicularis sp. was isolated from a paper mill and its EPS was produced on laboratory scale. After production, the exopolysaccharide (EPS) was purified and analysed for its purity and homogeneity, HPSEC revealed one distinct population with a molecular mass of more than 2,000 kDa. The protein content was around 9 w/w%. The sample was analysed to determine its chemical structure. The EPS was found to consist of rhamnose, glucose, galacturonic acid and glucuronic acid. Due to the presence of uronic acids the molar ratio between the four sugars found varies from 3:5:2:4 by sugar composition analyses after methanolysis to 1:1:1:1 found by NMR. A repeating unit with a molecular mass of 678 Da was confirmed by MALDI-TOF mass spectrometry after mild acid treatment. 13C and 1H hetero- and homonuclear 2D NMR spectroscopy of the native and partial hydrolysed EPS revealed a repeating unit, no non-sugar substituents were present.     

Incorporation of chlorohexidin diacetate into cotton fabrics grafted with glycidyl methacrylate and cyclodextrin

Linear electron beam radiation was used to induce grafting of glycidyl methacrylate/β-cyclodextrin mixture onto cotton fabric. Chlorohexidin diacetate was incorporated to the cavities of cyclodextrin fixed on the cotton fabric to form an inclusion complex having antimicrobial activity. After incorporating chlorohexidin diacetate, the fabric was subjected to several washing cycles to examine the durability of the antimicrobial finishing. Control and grafted cotton fabrics (before and after loading with antimicrobial agent) were characterized for their antimicrobial activity against different kinds of bacteria and fungi.Grafted fabrics loaded with antimicrobial agent were found to show good antimicrobial activity in comparison with control and grafted fabrics which are not loaded with antimicrobial agent. The grafted fabrics loaded with antimicrobial agent were found also to exhibit good antimicrobial activity after five washings and this lasting antimicrobial activity can be attributed to the inclusion complex formed between chlorohexidin diacetate molecules and the cavities of cyclodextrin.     

生物反应器规模化培养重组CHO-C28细胞表达HBsAg的研究

应用新型聚酯纤维盘片,采用连续灌注培养方式,分别试验了细胞接种量、pH、DO、罐流速度等因素对CHO-C28细胞生长分泌HBsAg的影响,初步建立了5L生物反应器生产重组乙型肝炎疫苗的生产工艺。经3次试验培养,每次培养60d,较适宜的培养条件确定为:pH6.80-7.10,DO 20%-30%,温度36-37℃,灌流速度138ml/h,接种浓度1.9×106cell/ml。收获液的HBsAg平均滴度是1∶256,最高滴度可达1∶512,纯化后的HBsAg产率为0.912mg/L。最后对反应器培养工艺与现行的转瓶培养工艺进行了比较,生物反应器培养具有可控制培养条件、不易污染和可使HBsAg产率提高等优点。     

麻栎壳斗染料在羊毛染色中的应用

本文研究了提取自麻栎壳斗的植物染料(麻栎染料)的耐酸、碱稳定性,染浴pH值及铝、铁离子等环保型媒染剂对其染毛织物效果的影响,并且探究了其染色动力学.研究表明,麻栎染料在强酸性染浴(pH=3)中对羊毛织物直接性好,染色后毛织物得棕色,也可采用铝离子、铁离子对直接染色后的毛织物进行后媒染,以得到不同色相的毛织物,尤其是铁后...     

绿僵菌无纺布菌条的制作(Ⅰ)液体种子培养条件

研究了金龟子绿僵菌菌株Ma83液体培养最佳营养及环境条件,为无纺布培养提供良好的液体种子.以生物量为指标,通过液体摇瓶试验确定了绿僵菌Ma83菌株液体种子培养参数,对不同的氮源、碳源及其浓度和初始pH进行单因素分析.结果表明,绿僵菌Ma83菌株液体种子培养最佳营养条件为4%黄豆粉,2%白砂糖,0.5%MgSO4·7H2O,0.05g/LKCl,0.1g/L FeSO4·7H2O,1.0g/L KH2PO4,pH6.3～6.5.70L发酵罐培养,干生物量第三天可达35g/L,显著优于筛选前培养基(SDA,28g/L,a=0.05).     

The Determination of Assay for Laccase of Bacillus subtilis WPI with Two Classes of Chemical Compounds as Substrates

Ligninolytic enzyme complexes are involved in lignin degradation. Among them laccases are outstanding because they use molecular oxygen as a co-substrate instead of hydrogen peroxide as used by peroxidases. Bacterial laccase of Bacillus genus was first reported in Claus and Filip (Microbiol Res 152:209–216, 1997), since then more bacterial laccases have been found. In this research, laccase-producing bacteria were screened from pulp and paper industry wastewater, bagass and sugarcane rhizosphere. Nutrient agar medium containing 0.5 mM of guaiacol was used. It was observed that the laccase-producing strains developed brown colour from which 16 strains of Bacillus were identified. One of the isolated strains was identified as Bacillus subtilis WPI based on the results of biochemical tests and 16S rDNA sequence analysis. This strain showed laccase-like activity towards the oxidizing substrates ABTS and guaiacol. In this study guaiacol was used as the substrate of laccase activity assay. For determination of laccase activity of this isolate guaiacol was used as a substrate of assay for the first time in this study. SDS-PAGE and Native-PAGE confirmed the presence of laccase.     

Effect of microwave irradiation on the physical properties and morphological structures of cotton cellulose

Microwave heating has been proved to be more rapid, uniform and efficient, and easily penetrate to particle inside. To investigate the effect of microwave irradiation on the physical property and morphological structure of cotton cellulose, cellulose fabric was treated with microwave irradiation at different conditions. The physical properties of the treated cellulose fabric were investigated. The morphological structures and thermal stabilities of the untreated and treated cellulose were investigated with differential scanning calorimetry (DSC) and X-ray diffraction. The results show that the physical properties of the treated cellulose fabrics were improved and the recoverability had not significant change. The thermal stability of the treated cellulose was changed. The crystallinity and preferred orientation of the treated cotton cellulose increased.     

Fruit Mineral Contents of Six Wild Species of the North Andean Patagonia, Argentina

The fruit mineral contents (K, Ca, Ba, Br, Zn, Co, Cr, Fe, Na, Rb, Cs, and Sr) of four native and two exotic naturalized shrubs growing in different areas of the Andean Patagonian region of Argentina were investigated. Native species Berberis darwinii, Berberis microphylla (Berberidaceae), Aristotelia chilensis (Elaeocarpaceae) and Ribes magellanicum (Saxifragaceae) produce small berries while the fruits of the exotic species Rosa rubiginosa and Rosa canina (Rosaceae) are aggregates of aquenes. They are used to prepare jams, tea, liquors, and ice creams. Native shrub fruits had higher content of Br, Zn, Co, Cr, Fe, Mo, and Na than those of the exotic naturalized species. Rosa species showed the highest contents of Ca and Ba in their fruits (the mean content doubled those of the native plant fruits). The fruit nutrient content found in the studied species was similar or higher than other values reported for fruits of temperate and tropical species in the world.