全文获取类型
收费全文 | 4553篇 |
免费 | 325篇 |
国内免费 | 393篇 |
出版年
2024年 | 14篇 |
2023年 | 70篇 |
2022年 | 93篇 |
2021年 | 147篇 |
2020年 | 140篇 |
2019年 | 167篇 |
2018年 | 178篇 |
2017年 | 124篇 |
2016年 | 125篇 |
2015年 | 142篇 |
2014年 | 235篇 |
2013年 | 271篇 |
2012年 | 151篇 |
2011年 | 184篇 |
2010年 | 178篇 |
2009年 | 218篇 |
2008年 | 220篇 |
2007年 | 224篇 |
2006年 | 225篇 |
2005年 | 220篇 |
2004年 | 192篇 |
2003年 | 163篇 |
2002年 | 162篇 |
2001年 | 130篇 |
2000年 | 95篇 |
1999年 | 73篇 |
1998年 | 91篇 |
1997年 | 95篇 |
1996年 | 61篇 |
1995年 | 59篇 |
1994年 | 59篇 |
1993年 | 46篇 |
1992年 | 59篇 |
1991年 | 48篇 |
1990年 | 38篇 |
1989年 | 50篇 |
1988年 | 49篇 |
1987年 | 36篇 |
1986年 | 26篇 |
1985年 | 44篇 |
1984年 | 60篇 |
1983年 | 72篇 |
1982年 | 71篇 |
1981年 | 56篇 |
1980年 | 34篇 |
1979年 | 39篇 |
1978年 | 12篇 |
1977年 | 6篇 |
1974年 | 5篇 |
1973年 | 5篇 |
排序方式: 共有5271条查询结果,搜索用时 15 毫秒
1.
《Molecular & cellular proteomics : MCP》2022,21(12):100438
Human pancreatic stellate cells (HPSCs) are an essential stromal component and mediators of pancreatic ductal adenocarcinoma (PDAC) progression. Small extracellular vesicles (sEVs) are membrane-enclosed nanoparticles involved in cell-to-cell communications and are released from stromal cells within PDAC. A detailed comparison of sEVs from normal pancreatic stellate cells (HPaStec) and from PDAC-associated stellate cells (HPSCs) remains a gap in our current knowledge regarding stellate cells and PDAC. We hypothesized there would be differences in sEVs secretion and protein expression that might contribute to PDAC biology. To test this hypothesis, we isolated sEVs using ultracentrifugation followed by characterization by electron microscopy and Nanoparticle Tracking Analysis. We report here our initial observations. First, HPSC cells derived from PDAC tumors secrete a higher volume of sEVs when compared to normal pancreatic stellate cells (HPaStec). Although our data revealed that both normal and tumor-derived sEVs demonstrated no significant biological effect on cancer cells, we observed efficient uptake of sEVs by both normal and cancer epithelial cells. Additionally, intact membrane-associated proteins on sEVs were essential for efficient uptake. We then compared sEV proteins isolated from HPSCs and HPaStecs cells using liquid chromatography–tandem mass spectrometry. Most of the 1481 protein groups identified were shared with the exosome database, ExoCarta. Eighty-seven protein groups were differentially expressed (selected by 2-fold difference and adjusted p value ≤0.05) between HPSC and HPaStec sEVs. Of note, HPSC sEVs contained dramatically more CSE1L (chromosome segregation 1–like protein), a described marker of poor prognosis in patients with pancreatic cancer. Based on our results, we have demonstrated unique populations of sEVs originating from stromal cells with PDAC and suggest that these are significant to cancer biology. Further studies should be undertaken to gain a deeper understanding that could drive novel therapy. 相似文献
2.
3.
Gender identification of shovelnose sturgeon using ultrasonic and endoscopic imagery and the application of the method to the pallid sturgeon 总被引:7,自引:0,他引:7
M. L. Wildhaber† D. M. Papoulias† A. J. DeLonay† D. E. Tillitt† J. L. Bryan† M. L. Annis† J. A. Allert† 《Journal of fish biology》2005,67(1):114-132
Monthly sampling of shovelnose sturgeon Scaphirhynchus platorynchus , a biological surrogate for the endangered pallid sturgeon Scaphirhynchus albus , was conducted to develop a multi‐seasonal profile of reproductive stages. Data collected included histological characteristics of gonads from wild caught fish and laboratory and field ultrasonic and endoscopic images. These data were used to compare effectiveness of ultrasonic and endoscopic techniques at identifying gender of adult shovelnose sturgeon at different reproductive stages. The least invasive method ( i.e . ultrasound) was least effective while the most invasive ( i.e . endoscope through an abdominal incision) was the most effective at identifying shovelnose sturgeon gender. In most cases, success rate for identifying males was greater than females, with success at identifying both genders greater in more advanced reproductive stages. Concomitantly, for most months average reproductive stage was more advanced for males than females. April and May were the months with the most advanced reproductive stage, and were the months when ultrasound was most effective. Methods were also applied in the Upper Missouri River to validate their use on pallid sturgeon Scaphirhynchus albus . Ultrasound was successful at identifying pallid sturgeon gender, however, endoscopic examination through the urogenital duct was only successful at identifying pallid sturgeon gender when the urogenital duct was not opaque. 相似文献
4.
5.
Colin K.W. Watts Robert L. Sutherland 《Biochemical and biophysical research communications》1984,120(1):109-115
Saturation and competitive binding analyses demonstrated the presence of a high affinity (KD = 0.92 nM), specific antiestrogen binding site (AEBS) in rat liver microsomes and at least 75% of total liver AEBS was recovered in this fraction. When microsomes were further separated into smooth and rough fractions, AEBS was concentrated in the latter. Subsequent dissociation of ribosomes from the rough membranes revealed that AEBS was associated with the membrane and not the ribosomal fraction. Antiestrogen binding activity could not be extracted from membranes with 1 M KCl or 0.5 M acetic acid but could be solubilized with sodium cholate. These data indicate that AEBS is an integral membrane component of the rough microsomal fraction of rat liver. 相似文献
6.
7.
Steven M. Melemis 《The Yale journal of biology and medicine》2015,88(3):325-332
There are four main ideas in relapse prevention. First, relapse is a gradual process with distinct stages. The goal of treatment is to help individuals recognize the early stages, in which the chances of success are greatest. Second, recovery is a process of personal growth with developmental milestones. Each stage of recovery has its own risks of relapse. Third, the main tools of relapse prevention are cognitive therapy and mind-body relaxation, which are used to develop healthy coping skills. Fourth, most relapses can be explained in terms of a few basic rules. Educating clients in these rules can help them focus on what is important: 1) change your life (recovery involves creating a new life where it is easier to not use); 2) be completely honest; 3) ask for help; 4) practice self-care; and 5) don’t bend the rules. 相似文献
8.
Lucilia sericata has a facultative diapause in the third larval instar after cessation of feeding. Induction of the diapause is influenced by the photoperiod and temperature conditions experienced by insects in the parental generation as well as those experienced by the larvae themselves. The sensitive stage of the parental generation for induction of diapause was examined using diapause‐averting conditions of 16 h light : 8 h darkness (LD 16:8) at 25°C and diapause‐inducing conditions of LD 12:12 at 20°C. The incidence of diapause in the progeny was predominantly determined by the conditions experienced by the parents in the adult stage. Moreover, the results of reciprocal crosses showed that only the mother's experience is involved in the induction of diapause in the progeny. 相似文献
9.
V. V. Galat 《Russian Journal of Developmental Biology》2000,31(1):1-8
Recent success in assisted fertilization mainly depended on the development of sperm microinjection methods: intracytoplasmic
sperm injection and subzonal insemination. Some basic mechanisms that under-lie fertilization were revealed by using intracytoplasmic
sperm injection. In respect to this, problems of fertility, oocyte activation, formation of pronuclei and practical aspects
of intracytoplasmic sperm injection are discussed. 相似文献
10.
Samuel M. Imathiu Rumiana V. Ray Matthew I. Back Martin C. Hare Simon G. Edwards 《Journal of Phytopathology》2013,161(7-8):553-561
Fusarium langsethiae is a toxigenic fungal species that has been reported in European small‐grain cereal crops such as oats, wheat and barley. Although its relative contribution to fusarium head blight (FHB) symptoms is not well understood, it is reported to contaminate these cereals with high levels of HT‐2 and T‐2 trichothecenes mycotoxins that are currently under consideration for legislation by the European Commission. Ten commercial oat fields in Shropshire and Staffordshire (two adjacent counties in the Midlands) in the UK were surveyed in the 2006/2007 growing season. Samples were taken from predetermined field locations at Zadoks growth stages 32/33, 69, 77‐85 and 90‐92 for F. langsethiae biomass and HT‐2 and T‐2 toxins quantification. The results from this study showed that oats can be heavily infected with F. langsethiae and have high concentrations of HT‐2 and T‐2 toxins with no apparent FHB symptoms. The regression of HT‐2 + T‐2 toxins on F. langsethiae DNA concentration was highly significant (P < 0.001, r2 = 0.55). The results indicated that although F. langsethiae had no direct effect on crop yield, it may result in indirect economic losses where the grain can be rejected or downgraded as a result of intolerable levels of HT‐2 and T‐2 toxins, which are of human food and animal feed safety concern. The influence of cultural field practices on the infection and HT‐2 and T‐2 toxins accumulation in oats was not clear and warrants further studies to identify the sources of F. langsethiae inoculum and conditions favourable for infection and mycotoxin production. 相似文献