受白粉菌诱导大麦抗感等基因系蛋白质变化的双向电泳分析

分别对接种与否的大麦抗—感白粉病等基因系—叶期幼苗取材进行蛋白质双向电泳分析。结果表明,病原的侵入使抗—感两系在30Kd以下的低分子量区域的蛋白质发生了明显变化。接种48小时之后,抗病系在pH5.5、6.0、6.8及8.8附近出现了对照中所没有的蛋白质,而在pH6.0和8.8附近的蛋白质则较对照有减小的趋势;感病系在pH6.0附近蛋白质明显增多,在pH8.8处不仅在量上有大幅度提高,而且种类也有增加。结果还表明,抗—感系间在未接种的情况下双向电泳图谱也有差异,接种之后由于感病系在pH8.8处蛋白质的特异性合成,使抗—感两系间的差异缩小。     

Effects of sowing date and vernalisation on the growth of winter barley and its resistance to powdery mildew (Erysiphe graminis f.sp. hordei)

Detailed studies on the production of individual leaves, and the development of powdery mildew on them, were made in field plots of winter barley sown on different dates. The greater severity of the disease on early-sown than on later-sown seedlings during the autumn and winter can probably be explained mainly by changes in the abundance of inoculum and the suitability of the weather for infection. Results from glasshouse experiments suggest that the differences may be reinforced by direct effects of vernalisation on the susceptibility of seedlings to the disease. Contrary effects of sowing date on mildew severity during summer are probably due to the progressively greater resistance to mildew of the later-formed than of seedling leaves, and the earlier appearance of corresponding leaves on early-sown than on later-sown plants. Early sowing can also increase the total number of leaves produced per stem. Therefore, because resistance of the leaves increases progressively, the maximum degree of resistance expressed by the later-formed (e.g. flag) leaves will often be greater on early-sown than on later-sown plants.     

禾谷多粘菌游动孢子超微结构观察

本文描述了寄生在大麦根部的禾谷多粘菌Polymyxa graminis Led.的次生游动孢子的超微结构,包括核、内质网、高尔基体、线粒体、脂质粒、排泄泡、小囊、具膜小囊、核糖体以及鞭毛基体(Kinetosome)和鞭毛杆等细胞器。游动孢子中未见微体。同时也在电镜下观察了游动孢子接触和穿透根细胞时所形成的管腔(Rohr)和棘杆(Stachel)以及游动孢子穿透细胞壁的详细过程。