Polycyclic polyprenylated acylphloroglucinol derivatives from Hypericum scabrum

Three new polycyclic polyprenylated acylphloroglucinol derivatives (PPAPs), hyperibrins E–G (1–3), along with seven known compounds were identified from the air-dried aerial parts of Hypericum scabrum. Their structures were determined by NMR spectroscopic methods, both experimental and calculated electronic circular dichroism (ECD) spectra and comparison with known compounds. Compound 1 was derived from an analogue of compound 2 by cyclization, while a retro-Claisen reaction transformed another analogue of compound 2 into compound 3. Compounds 3, 4, 5, and 10 showed obvious hepatoprotective activities against paracetamol-induced HepG2 cell damage.     

Polycyclic Polyprenylated Acylphloroglucinols and Chromone O‐Glucosides from Hypericum henryi subsp. uraloides

Two new C(30)‐epimeric polycyclic polyprenylated acylphloroglucinols (PPAPs), named uralodins B and C ( 1 and 2 , resp.), were isolated from the aerial parts of Hypericum henryi subsp. uraloides together with two new chromone glucosides, urachromones A and B ( 3 and 4 , resp.), as well as 16 known compounds. Their structures were established by extensive NMR techniques and MS analysis. The epimers 1 and 2 always behaved like a single compound when examined by TLC, and were separated by HPLC. Their configuration was distinguished by comparative analysis of the NMR data with known analogues together with the ROESY experiment. All the isolated PPAPs were evaluated for their cytotoxic activities against HepG2, SGC7901, HL‐60, and K562 cell lines. Compound 1 showed modest cytotoxic activities against SGC7901 and HL‐60 cell lines, and 2 showed modest cytotoxic activities against HepG2, SGC7901, HL‐60, and K562 cell lines.     

纤枝金丝桃化学成分研究北大核心CSCD

为了探究滇产植物纤枝金丝桃的物质基础,寻找活性化合物,该文用80%乙醇对纤枝金丝桃地上部分浸渍提取,应用HP-20大孔吸附树脂、硅胶、葡聚糖凝胶、半制备高效液相等色谱技术对纤枝金丝桃的化学成分进行分离纯化,根据波谱数据鉴定化合物的结构。结果表明:(1)从纤枝金丝桃中分离得到15个化合物,分别鉴定为attenuatumione G(1)、uralodin B(2)、chipericumin C(3)、2,5-二羟基-1-甲氧基氧杂蒽酮(4)、1,7-二羟基氧杂蒽酮(5)、1,7-二羟基-4-甲氧基氧杂蒽酮(6)、槲皮苷(7)、芹菜素-7-O-β-D-葡萄糖苷(8)、芹菜素-7-O-β-D-(6″-O-乙酰基)-葡萄糖苷(9)、木犀草素(10)、槲皮素(11)、白桦脂酸(12)、白桦脂酸甲酯(13)、白桦脂酮酸(14)、β-谷甾醇(15)。化合物1-14为首次从该植物中分离得到。(2)采用MTT法对化合物1-14进行体外抗乳腺癌活性测试,结果仅显示化合物3、6、13对2种乳腺癌细胞株MCF-7和MDA-MB-231有一定的抑制作用,其IC_(50)值为48.6~123.5μg·mL^(-1)。该研究结果对综合开发利用纤枝金丝桃资源具有理论和应用意义。