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1.
A new brain protein is described which forms an insoluble complex with tubulin, with concomitant stoichiometric hydrolysis of GTP. The complex contains a maximum of one tubulin-binding protein (MW 52,500) per two tubulin dimers. The tubulin-binding protein (TBP) does not compete with colchicine, but in the presence of microtubule-associated proteins tubulin appeared less accessible to it. Proteins such as TBP might sequester tubulin and thereby function either to inhibit indiscriminate polymerization, or to promote ordered nucleation by maintaining high local concentrations. 相似文献
2.
Pavel Veprek Jan Jezek Jirí Velek Hatem Tallima Mona Montash Rashika El Ridi 《Journal of peptide science》2004,10(6):350-362
Four monoepitopic MAPs (MAP A, B, C and E) and one bis-diepitopic MAP B-E derived fromthe primary sequence of Schistosoma mansoni glyceraldehyde 3-phosphate dehydrogenase, previously tested in BALB/c mice, were examined for their immunogenicity and protective capacity in C57BL/6 mice. Despite multimerization into MAPs, MAP Aand MAP C were poorly immunogenic. In contrast toBALB/c mice, MAP E was non-immunogenic in C57BL/6 mice. Peptide B in the form of MAP B orbis-diepitopic MAPB-E elicited immune responses in C57BL/6 mice that were associated with a significant decrease in worm burden. The MAPs were prepared by the stepwise solid-phase peptide synthesis using Boc/Bzl chemistry, successfully purified on the RP-HPLC column and characterized by RP-HPLC, HPCE and MALDI-TOF MS techniques. A general strategy for MAPs purification is discussed here and the purification of MAP Band MAP E is documented in detail. 相似文献
3.
Flemming Jessen Bruce D. Cherksey Thomas Zeuthen Else K. Hoffmann 《The Journal of membrane biology》1989,108(2):139-151
Summary Furosemide-binding proteins were isolated from cholate-solubilized membranes of Ehrlich ascites tumor cells by affinity chromatography, using furosemide as ligand. Solubilized proteins retarded by the affinity material were eluted by furosemide. In reducing and denaturing gels, the major proteins eluted by furosemide were 100 and 45 kDa. In nonreducing, nondenaturing gels, homodimers of both polypeptides were found, whereas no oligomeric proteins containing both polypeptides were seen. It is concluded that the furosemide gel binds two distinct dimeric proteins. The isolated proteins were reconstituted into phospholipid vesicles and the K+ transport activity of these vesicles was assayed by measurement of86Rb+ uptake against a large opposing K+ gradient. The reconstituted system was found to contain a K+ transporting protein, which is sensitive to Ba2+ like the K+ channel previously demonstrated to be activated in intact cells after cell swelling. 相似文献
4.
伤寒Vi多糖菌苗接种反应观察 总被引:3,自引:1,他引:2
本文报告了对我国首次成功的伤寒Vi多糖菌苗进行人体接种反应观察结果,接种对象为20至54岁无伤寒病史,近年无伤寒菌苗接种史的健康人,共60名,以完全随机的方法分为两组,实验组注射30μgVi多糖菌苗,对照组注射Vi多糖菌苗的稀释液。其结果30名Vi多糖菌苗接种者注射后体温无中重反应发生,局部红肿仅有1例中反应。注射后对血压、心律没有影响。红细胞计数,白细胞计数均在正常范围,与接种前相比,无显著差异 相似文献
5.
对79名HBV标志阴性少年人群,以乙肝疫苗10μg×3的免疫剂量和0、1、2月的免疫程序进行接种,对其抗-HBs免疫应答和临床保护效果作了为期7年的定人随访。结果表明,抗-HBs阳转率在免后三个月时为100%,均值为3084MIU/ml。至免后84个月时疫苗接受者中仍有55.7%的抗-HBs水平≥10MIU/ml。6例检出抗-HBs,其中5例的抗-HBs持续处于高水平。全部观察对象无一例检出HBsAg或发生临床肝炎。少年接种乙肝血源疫苗至少7年内可具有保护性抗体。故在此期内不需加强免疫。 相似文献
6.
不同月龄婴儿接种DTP后血清中百日咳抗体水平的观测 总被引:2,自引:1,他引:1
本文对比观察了213例2月龄、3月龄婴儿接种DTP后百日咳抗体水平的变化,探讨了母传抗体对免后抗体增长的影响。结果表明2月龄、3月龄婴儿DTP免疫后1个月和3个月血清中百日咳抗体达保护水平的百分率无显著性差异(x ̄2=0.036,p>0.9;x ̄2=0.327,p>0.5),免后1个月抗体GMT无显著性差异(t=0.17,p>0.5),免后3个月抗体GMT3月龄组高于2月龄组(t=2.22,p<0.05)。我们还发现免前抗体水平与免后1个月GMT虽有负相关(r=—0,754)的倾向,但总体上对抑制免后抗体应答不明显,因而建议将儿童DTP基础免疫的起始月龄提前至2月龄进行。 相似文献
7.
Bahman Tabaraie Bal Krishan Sharma Praveen Rishi nee Sharma Rakesh Sehgal Nirmal Kumar Ganguly 《Microbiology and immunology》1994,38(7):553-559
Porins were prepared from smooth strain of Salmonella typhi 0–901 and chemotype of rough mutant of S. typhimurium Ra-30. Mice were immunized with both the porin preparations in different groups and challenged with S. typhimurium LT2–71 and S. enteritidis SH-1269. Porin immunized mice showed significant protection (P <0.01) against challenge with homologous as well as heterologous strains. Hence, the use of porins may be attempted in future to protect against salmonellosis. 相似文献
8.
Per Putkonen Charlotta Nilsson Kerstin Hild Reinhold Benthin Martin Cranage Anne Marie Aubertin Gunnel Biberfeld 《Journal of medical primatology》1993,22(2-3):100-103
Several groups have reported protection against experimental SIV infection in macaques immunized with a whole inactivated virus vaccine. The aim of the current study was to investigate whether five macaques vaccinated with whole inactivated SIV and previously shown to be protected against challenge with two divergent strains of SIV grown on human cells could resist challenge with a subsequent homologous SIV grown on macaque cells. We show here that this same vaccine did not protect when the challenge virus was grown on primary cells of monkey origin. 相似文献
9.
本文对吸附精制百白破混合制剂的不同配方进行了实验,结果表明,新一代吸附精制百白破混合制剂最佳配方为:精制百日咳菌苗18μgPN/ml、精制白喉类毒素为30Lf/ml、精制破伤风类毒素为10Lf/ml。由该配方组成的吸附精制百白破混合制剂,其中百日咳菌苗的毒性试验BWDU/ml、LPU/ml、HSU/ml三种指标均符合制检规程要求。其效力单位(IU/ml)超过规程要求一倍以上,精制白喉和破伤风类毒素的安全试验均符合规程要求,白类效力试验≥80-100%,破类效力试验≥0.5-4.5IU/ml。上述结果说明本文提出的配方作为新一代精制百白破混合制剂的配方是适宜和实用的。 相似文献
10.
Henipaviruses and lyssaviruses target nucleolar treacle protein and regulate ribosomal RNA synthesis
Stephen M. Rawlinson Tianyue Zhao Katie Ardipradja Yilin Zhang Patrick F. Veugelers Jennifer A. Harper Cassandra T. David Vinod Sundaramoorthy Gregory W. Moseley 《Traffic (Copenhagen, Denmark)》2023,24(3):146-157
The nucleolus is a common target of viruses and viral proteins, but for many viruses the functional outcomes and significance of this targeting remains unresolved. Recently, the first intranucleolar function of a protein of a cytoplasmically-replicating negative-sense RNA virus (NSV) was identified, with the finding that the matrix (M) protein of Hendra virus (HeV) (genus Henipavirus, family Paramyxoviridae) interacts with Treacle protein within nucleolar subcompartments and mimics a cellular mechanism of the nucleolar DNA-damage response (DDR) to suppress ribosomal RNA (rRNA) synthesis. Whether other viruses utilise this mechanism has not been examined. We report that sub-nucleolar Treacle targeting and modulation is conserved between M proteins of multiple Henipaviruses, including Nipah virus and other potentially zoonotic viruses. Furthermore, this function is also evident for P3 protein of rabies virus, the prototype virus of a different RNA virus family (Rhabdoviridae), with Treacle depletion in cells also found to impact virus production. These data indicate that unrelated proteins of viruses from different families have independently developed nucleolar/Treacle targeting function, but that modulation of Treacle has distinct effects on infection. Thus, subversion of Treacle may be an important process in infection by diverse NSVs, and so could provide novel targets for antiviral approaches with broad specificity. 相似文献