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R. W. Thornburg C. Carter A. Powell R. Mittler L. Rizhsky H. T. Horner 《Plant Systematics and Evolution》2003,238(1-4):211-218
We have characterized the major nectar protein (Nectarin I) from ornamental tobacco as a superoxide dismutase that functions
to generate high levels of hydrogen peroxide in nectar. Other nectar functions include an anti-polygalacturonase activity
that may be due to a polygalacturonase inhibiting protein (PGIP). We also examined the expression of defense related genes
in the nectary gland by two independent methods. We isolated a sample of nectary-expressed cDNAs and found that 21% of these
cDNAs were defense related clones. Finally, we examined the expression of a number of specific defense-related genes by hybridization
to specific cDNAs. These results demonstrated that a number of specific defense genes were more strongly expressed in the
floral nectary than in the foliage. Taken together these results indicate that the floral nectary gland can have specific
functions in plant defense.
Received August 8, 2002; accepted January 7, 2003
Published online: June 2, 2003 相似文献
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M. Manuela Ribeiro Costa Júlia Costa Cândido P. Pinto Ricardo 《Physiologia plantarum》1997,99(2):263-270
A glycoprotein with an apparent molecular mass of 42 kDa (GP42), detected in the roots of Lupinus albus L. (cv. Rio Maior), was found to increase along the root axis with increasing distance from the apex and to be induced in roots cultured in vitro upon exogenous supply of high IAA (10-3 M ). GP42 is ionically bound to the cell wall, it has a pl>8.3, and it is N -glycosylated. The purification of GP42 was accomplished by affinity chromatography (ConA-Sepharose) followed by cation-exchange chromatography (Mono S). The amino acid sequence of the amino terminal part of the protein shows 70% identity to that of polygalacturonase inhibitor proteins (PGIPs) from other species. GP42 inhibits the polygalacturonase activity from Aspergilltus niger in vitro suggesting that it is a PGIP. The possible relationship between the L. albus PGIP and root development is discussed. 相似文献
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Hamed Doostdar T.Gregory McCollum Richard T Mayer 《Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology》1997,118(4):861-867
An endo-polygalacturonase (PG) (EC:3.2.1.15) with a pI of 9.4 and an Mr of 44,500 was purified to electrophoretic homogeneity from the gut of West Indies sugarcane rootstalk borer weevil (Diaprepes abbreviatus L.) larvae. Hydrolytic activity was maximal in 150 mM sodium acetate, pH 5.5, at 30°C. Kinetic determinations yielded an apparent Km of 3.68 mg polygalacturonic acid (PGA)/ml and a Vmax of 283 μmol galacturonic acid/min/mg protein for PGA. Enzymatic activity was inhibited by a polygalacturonase inhibitor protein from “Hamlin” orange flavedo. The purified protein does not appear to be glycosylated, and its N-terminal sequence showed no homology to any PG protein sequences in data banks. 相似文献
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Protsenko MA Buza NL Krinitsyna AA Bulantseva EA Korableva NP 《Biochemistry. Biokhimii?a》2008,73(10):1053-1062
It is generally believed that plants "evolved a strategy of defending themselves from a phytopathogen attack" during evolution. This metaphor is used frequently, but it does not facilitate understanding of the mechanisms providing plant resistance to the invasion of foreign organisms and to other unfavorable external factors, as well as the role of these mechanisms in plant growth and development. Information on processes involving one of the plant resistance factors--polygalacturonase-inhibiting protein (PGIP)--is considered in this review. The data presented here indicate that PGIP, being an extracellular leucine-rich repeat-containing protein, performs important functions in the structure of plant cell wall. Amino acid residues participating in PGIP binding to homogalacturonan in the cell wall have been determined. The degree of methylation and the mode of distribution of homogalacturonan methyl groups are responsible for the formation of a complex structure, which perhaps determines the specificity of PGIP binding to pectin. PGIP is apparently one of the components of plant cell wall determining some of its mechanical properties; it is involved in biochemical processes related to growth, expansion, and maceration, and it influences plant morphology. Polygalacturonase (PG) is present within practically all plant tissues, but the manifestation of its activity varies significantly depending on physiological conditions in the tissue. Apparently, the regulation of PG functioning in apoplast significantly affects the development of processes associated with the modification of the structure of plant cell wall. PGIP can regulate PG activity through binding to homogalacturonan. The genetically determined structure of PGIP in plants determines the mode of its interaction with an invader and perhaps is one of the factors responsible for the set of pathogens causing diseases in a given plant species. 相似文献
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马哈利樱桃PGIP cDNA克隆序列分析 总被引:3,自引:1,他引:2
以马哈利樱桃(Prunus mahaleb L.)为材料,通过RT-PCR获得了1045bp的目的段,经克隆测序,证实该片段包含1个完整的开放阅读框架,该阅读框架由990碱基组成,编码330个氨基酸。该序列与杏、梨、苹果的PGIP cDNA序列同源性分别达97.2%、83.4%和83.6%,可能编码的氨基酸与杏、梨、苹果的PGIP cDNA所编码的氨基酸的同源性分别达到96.7%、85.2%和85.2%。与已经克隆的PGIP DNA序列的对比分析表明,PGIP DNA序列中包含2个外显子和1个内含子,内含子全长147 ,符合TG-AG规律,2个外显子长度分别为581bp、464bp。 相似文献
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九台晚李PGIP基因的克隆及生物信息学分析 总被引:1,自引:0,他引:1
以九台晚李叶片基因组为模板,PGIP基因保守序列设计引物,PCR扩增到1条全长1192bp的目的片段(GenBank登录号:GU068978)。该基因包含有1个完整的开放阅读框,由2个外显子和1个内含子构成,外显子总长990bp,编码330个氨基酸,其编码的氨基酸序列中含有一段典型的亮氨酸重复序列。序列分析表明:该基因与中国李、杏、桃、马哈利樱桃、梅等李属植物的PGIP基因序列一致度达95%~99%。系统进化分析显示出属内亲缘关系较近、属间亲缘关系较远的特点。该序列为植物分子抗病育种提供了1条新的基因资源。 相似文献
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小麦内切多聚半乳糖醛酸酶抑制蛋白的分离纯化研究 总被引:9,自引:0,他引:9
小麦内切多聚半乳糖醛酸酶抑制蛋白的分离纯化研究郑远旗,杨宗剑,李建吾,周立,吴文莲(四川大学生物系,成都610064)余露(中国科学院成都生物研究所,成都610041)关键词内切多聚半乳糖醛酸酶抑制蛋白;内切多聚半乳糖醛酸酶;纯化;小麦内切多聚半乳糖... 相似文献
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Richter A Jacobsen HJ de Kathen A de Lorenzo G Briviba K Hain R Ramsay G Kiesecker H 《Plant cell reports》2006,25(11):1166-1173
The pea (Pisum sativum L.) varieties Baroness (United Kingdome) and Baccara (France) were transformed via Agrobacterium tumefaciens-mediated gene transfer with pGPTV binary vectors containing the bar gene in combination with two different antifungal genes coding for polygalacturonase-inhibiting protein (PGIP) from raspberry (Rubus idaeus L.) driven by a double 35S promoter, or the stilbene synthase (Vst1) from grape (Vitis vinifera L.) driven by its own elicitor-inducible promoter. Transgenic lines were established and transgenes combined via conventional crossing. Resveratrol, produced by Vst1 transgenic plants, was detected using HPLC and the PGIP expression was determined in functional inhibition assays against fungal polygalacturonases. Stable inheritance of the antifungal genes in the transgenic plants was demonstrated. 相似文献