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Various components of culture media were tested to characterize factors affecting plantlet regeneration from rice (Oryza sativa L.) callus. It was found that plantlet regeneration from rice callus was affected by concentrations of gelling agents, osmoticum,
and the combination of hormones in the regeneration medium. High concentrations (4–6 g/l gellan gum, 10–16 g/l agar) of gelling
agents promoted regeneration frequency. However, the total number of plantlets decreased with gellan gum concentrations above
4 g/l. Addition of sorbitol (15–75 g/l) promoted plantlet regeneration. However, the addition of mannitol was inhibitory and
no regeneration was observed at concentrations above 30 g/l. This difference in the effects on regeneration suggests that
sorbitol had another function besides as a osmoticum. High regeneration frequency was obtained with combinations of NAA (0.05–0.5
g/l) and kinetin (0.5–2 mg/l). However, higher concentrations (2 mg/l) of NAA are preferred to increase the total number of
regenerated plantlets. 相似文献
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碱性盐胁迫下星星草幼苗中几种渗透调节物质的变化 总被引:27,自引:2,他引:25
模拟松嫩盐碱草地碱化土壤的离子组成配制碱性盐溶液处理星星草(Puccinelliatenuiflora)幼苗,测定了碱性盐胁迫下星星草幼苗地上部分几种渗透调节物质含量的变化,随着碱性协浓度及胁迫天数的增加,Na^+可溶性盐,游离脯氨酸和可淀粉糖的含量逐渐增加,其中游离脯氨酸的变化倍数最大,K^+含量在浓度小于20gL^-1碱性胁迫下随胁迫天数增加而略为减少,在40grL^-1和60gL^-1碱性胁 相似文献
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