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1.
Merc Torres Jonh Jairo M ndez Ventura Sanahuja Ramon Canela 《Biocatalysis and Biotransformation》2003,21(3):129-134
We carried out reactive extractions at moderate temperatures using Rhizopus oryzae resting cells and various solid materials containing different proportions of vegetable oil. Our methodology allows for free fatty acids and either methyl or solketal fatty esters to be prepared in moderate to high yields. Moreover, the process can be carried out in either a solvent or a solvent-free system. In a solvent-free system, the final yield can be increased if easily ground solid material is used. The water present in each material and the solvents used has an influence on the results. 相似文献
2.
Luo-Ye Li Ling Wang Jin-Xue Jing Zhen-Qi Li Fei Lin Li-Fei Huang Qing-Hua Pan 《Molecular breeding : new strategies in plant improvement》2007,20(2):179-188
The Pik
m
gene in rice confers a high and stable resistance to many isolates of Magnaporthe oryzae collected from southern China. This gene locus was roughly mapped to the long arm of rice chromosome 11 with restriction
fragment length polymorphic (RFLP) markers in the previous study. To effectively utilize the resistance, a linkage analysis
was performed in a mapping population consisting of 659 highly susceptible plants collected from four F2 populations using the publicly available simple sequence repeat (SSR) markers. The result showed that the locus was linked
to the six SSR markers and defined by RM254 and RM144 with ≈13.4 and ≈1.2 cM, respectively. To fine map this locus, additional
10 PCR-based markers were developed in a region flanked by RM254 and RM144 through bioinformatics analysis (BIA) using the
reference sequence of cv. Nipponbare. The linkage analysis with these 10 markers showed that the locus was further delimited
to a 0.3-cM region flanked by K34 and K10, in which three markers, K27, K28, and K33, completely co-segregated with the locus.
To physically map the locus, the Pik
m
-linked markers were anchored to bacterial artificial chromosome clones of the reference cv. Nipponbare by BIA. A physical
map spanning ≈278 kb in length was constructed by alignment of sequences of the clones anchored by BIA, in which only six
candidate genes having the R gene conserved structure, protein kinase, were further identified in an 84-kb segment. 相似文献
3.
本文利用聚乙二醇和磷酸盐组成的水溶液双相系统,从米曲霉(Aspergillus oryzae)中提取氨基酰化酶。通过实验对影响氨基酰化酶分配的各参数进行了研究,确定了最适体系:PEC-1540 10%(W/V),K_2HPO_418%(W/V),pH8.5;PEG-154010%(W/V),K_2HPO_412%(W/V),NaCl 0.5mol/L,pH8.5。二步萃取收率90%,纯化倍数9。为实验室和工业上采用双水相系统萃取氨基酰化酶提供了一个新方法。 相似文献
4.
A. Ouazzani Touhami B. Ennaffah M. El Yachioui et A. Douira 《Journal of Phytopathology》2000,148(4):221-226
A comparative study into the pathogenicity of four Helminthosporiumspp. towards rice cultivars in Marocco In this paper, the behaviour of four Helminthosporium species on nine rice cultivars widely cultivated in Morocco, permit the classification of this species according to their virulence. Helminthosporium oryzae and Helminthosporium sativum are the most virulent and the best equipped to sporule on all varieties of tested lesions. The level of pathogenicity of Helminthosporium spiciferum and Helminthosporium australiensis and their capacity to sporule on host plant depend on the rice variety used. At the same time, each Helminthosporium induces characteristics symptoms on rice leaves. 相似文献
5.
OsSERK1 regulates rice development but not immunity to Xanthomonas oryzae pv. oryzae or Magnaporthe oryzae 总被引:1,自引:0,他引:1
Shimin Zuo Xiaogang Zhou Mawsheng Chen Shilu Zhang Benjamin Schwessinger Deling Ruan Can Yuan Jing Wang Xuewei Chen Pamela C. Ronald 《植物学报(英文版)》2014,56(12):1179-1192
Somatic embryogenesis receptor kinase (SERK) proteins play pivotal roles in regulation of plant development and immunity. The rice genome contains two SERK genes, OsSerk1 and OsSerk2. We previously demonstrated that OsSerk2 is required for rice Xa21-mediated resistance to Xanthomonas oryzae pv. oryzae (Xoo) and for normal development. Here we report the molecular characterization of OsSerk1. Overexpression of OsSerk1 results in a semi-dwarf phenotype whereas silencing of OsSerk1 results in a reduced angle of the lamina joint. OsSerk1 is not required for rice resistance to Xoo or Magnaporthe oryzae. Overexpression of OsSerk1 in OsSerk2-silenced lines complements phenotypes associated with brassinosteroid (BR) signaling defects, but not the disease resistance phenotype mediated by Xa21. In yeast, OsSERK1 interacts with itself forming homodimers, and also interacts with the kinase domains of OsSERK2 and BRI1, respectively. OsSERK1 is a functional protein kinase capable of auto-phosphorylation in vitro. We conclude that, whereas OsSERK2 regulates both rice development and immunity, OsSERK1 functions in rice development but not immunity to Xoo and M. oryzae. 相似文献
6.
Takeshi Yamaguchi Yumiko Maehara Osamu Kodama Mitsuo Okada Masatoshi Matsumura Naoto Shibuya 《Journal of plant physiology》2002,159(10):1147
Two purified oligosaccharide elicitors generatable from fungal cell walls, N-acetylchitoheptaose and a tetraglucosyl glucitol from rice blast fungus (Magnaporthe grisea), synergistically activated phytoalexin biosynthesis in cultured rice cells. Inhibition experiments for the binding of radiolabeled N-acetylchitooligosaccharide elicitor to the plasma membrane from rice cells indicate that the two elicitors are recognized by different receptors. These results also indicate the presence of a positive interaction between the signal transduction cascade downstream of each elicitor/receptor, which enhances resistance against pathogens. 相似文献
7.
利用5个小麦亲本,按Griffing方法4组配一套完全双列杂交,研究小麦籽粒蛋白质组分的配合力和遗传力。结果表明,同一性状不同亲本的一般配合力效应和不同组合间的特殊配合力效应差异都较大。球蛋白、醇溶蛋白和谷蛋白含量的遗传主要受加性基因控制,清蛋白含量的遗传不存在加性基因作用,以显性基因作用为主。
Abstract: A set of diallel crosses involving 5 wheat parents, according to the random model of Griffing Method 4, was made to study the combining ability and heritability of grain protein components in wheat. The results indicated that GCA effects of different parents for the same trait varied significantly. And SCA effects of different combination studied varied obviously. The inheritance of globulin, gliadin and glutenin contents were mainly controlled by additive genes. Dominant genes functioned mainly on albumin content without additive gene. 相似文献
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10.
Toshifumi Miyazawa Makoto Hiramatsu Takashi Murashima Takashi Yamada 《Biocatalysis and Biotransformation》2013,31(3):93-100
We examined Aspergillus melleus protease (Amano protease P) and A. oryzae protease (Amano protease A) as catalysts for peptide bond formation via the kinetically controlled approach. As the coupling efficiency was only moderate, even with a good amino acid substrate as the carboxyl component, in acetonitrile as a solvent (with or without a small amount of added water) that we had mainly employed previously in α-chymotrypsin catalyzed couplings, other solvent systems were sought. In 1,1,1,3,3,3-hexafluoro-2-propanol-DMF (1:1) without added water, these Aspergillus proteases were found to remain active for a long period of time and to be utilizable for peptide synthesis when the carbamoylmethyl ester was employed as the acyl donor, though the coupling efficiencies were dependent rather largely on the combination of the amino acid residues at the coupling site. The superiority of the carbamoylmethyl ester to conventional esters, for example the methyl ester, was once again established. Furthermore, some segment condensations were also achieved by the same procedure. 相似文献