Abstract Extended survival of Legionella pneumophila , using both a clinical and an environmental isolate, was studied in drinking water, creek water, and estuarine water microcosms. Legionella populations were monitored by acridine orange direct counts (AODC) and viable count on buffered charcoal yeast extract agar amended with alpha-ketoglutarate (BCYEα). Initial colony counts of the clinical isolate in drinking and creek water microcosms were 2 × 108 cfu/ml and, after incubation for 1.5 years, the plate counts decreased to 3 × 106 cfu/ml. The AODC counts, however, did not change significantly. The clinical isolate in estuarine water decreased in plate counts to 102 (cfu/ml) over the same period. After incubation for 1.5 years at 15°C in the microcosms, Legionella plate counts of creek and drinking water decreased by two logs. Direct microscopic examination of aliquots removed from all microcosms revealed the presence of small bacilli, large bacilli and rare filamentous cells. The environmental isolate demonstrated only one colony morphology upon culture on BCYEα. Interestingly, after four months incubation in the microcosm, upon plating the clinical isolate on BCYEα, two distinct colony types were evident. Examination by immunofluorescent staining employing a monoclonal antibody against L. pneumophila revealed both bacillus and filamentous forms. The total cellular proteins of both morphotypes were examined by sodium dodecyl sulfate polyacrylyamide gel electrophoresis (SDS-PAGE), demonstrating identical protein patterns. Those Legionella cells remaining culturable during 1.5 years of incubation grew rapidly when transferred to BCYEα. Incubation was continued and it was found that some strains of L. pneumophila serogroup 1 can remain viable for longer than 2.4 years under low-nutrient conditions. 相似文献
Abstract The process of colony formation by bacteria from grassland soil sampled in April, July and September was simulated by a colony-forming curve (CFC). The CFC was a super-imposition of several component curves (cCFC) given theoretically by the first order reaction (FOR) model [3,6]. The pattern of FOR model curves was not influenced by the time of sampling and four cCFCs were always recognized during an incubation period of 160 h. It was considered that the CFC describes an inherent property of the bacterial population of the field. Bacterial isolates were obtained from colonies produced in each of four cCFCs on agar plates. Isolates corresponding to one cCFC were classified as one group. The bacterial isolates were characterized by morphological and physiological tests and subsequently clustered. Few oligotrophic bacteria were obtained among bacteria which produced visible colonies within 63 h of incubation time. On the other hand, approx. 50% of bacteria which produced v colonies after 63 h were oligotrophic bacteria. The time required for the appearance of the first colony, t r of the FOR model, was very similar in the isolates belonging to one group. A close linear relationship was observed between t r value and doubling time of isolates. 相似文献
Synopsis Ages determined by counts of apparent annuli on scales, sagittae, vertebrae, pectoral fin ray and dorsal fin spine cross sections
of largemouth bass from northern populations, which are older and slower growing fish than in the southern parts of its native
range, were compared to establish the accuracy of each method. Linear regression techniques indicated strong agreement (r>
0.9) among ages assigned from the examination of scales, sagittae, and vertebrae. The pattern of growth zones on pectoral
fin ray and dorsal fm spine cross sections proved too variable for accurate age determination. Limited data suggest that ages
greater than 7+ assigned from scales were more likely to underestimate true ages than the other body parts used, although
none of these methods gave satisfactory results. Examination of scales from recovered tagged fish, and the similarity between
back-calculated lengths of fish through age 7+ to annulus I and observed lengths of juvenile largemouth bass near the end
of their first growing season, support the validity of ages determined from scales.
Despite a very limited amount of habitat suitable for largemouth bass and severe climatic conditions, growth of this species
in Tadenac Lake was similar to growth in other waters north of the Great Lakes. Differences in physical characteristics among
these waters does not appear to influence growth rates of largemouth bass, but probably affects production and biomass. 相似文献
It was investigated whether quorum sensing (QS) mediated by N-acylhomoserine lactones (AHLs) was important for heterotrophic bacteria from the littoral zone of the oligotrophic Lake Constance
for growth with organic particles. More than 900 colonies from lake water microcosms with artificial organic aggregates consisting
of autoclaved unicellular algae embedded in agarose beads were screened for AHL-production. AHL-producing bacteria of the
genus Aeromonas enriched in the microcosms but AHLs could not be detected in any microcosm. To test for a potential function of AHL-mediated
QS, growth experiments with the wild type and an AHL-deficient mutant of Aeromonas hydrophila in lake water microcosms were performed. Growth of both strains did not differ in single cultures and showed no mutual influence
in co-cultures. In co-cultures with a competitor bacterium belonging to the Cytophaga–Flavobacterium group, growth of both A. hydrophila strains was reduced while growth of the competitor bacterium was not affected. Exogenous AHL-addition did not influence growth
of the Aeromonas strains in any microcosm experiment. These results showed that AHL-mediated QS was not required for A. hydrophila during colonization and degradation of organic particles in lake water microcosms, suggesting that cell–cell signalling of
heterotrophic bacteria in oligotrophic waters relies on novel signal molecules. 相似文献
In this study the effect of ontogenetic drift on crassulacean acid metabolism (CAM) was investigated in the aquatic CAM-isoetid Littorella uniflora. The results of this study strengthen the general hypothesis of CAM being a carbon-conserving mechanism in aquatic plants, because high-CAM capacity (45–183 μequiv. g−1 FW) was present in all leaves of L. uniflora irrespective of age. Since possession of CAM in aquatic plants allows CO2 uptake throughout the light/dark cycle, presence of CAM in all leaves influences the carbon balance of L. uniflora positively. On average for all lakes, different leaf classes accounted for 11–36% of the total dark CO2 uptake by the individual plant.
The capacity for both CAM and photosynthesis declined with increasing leaf age, and was in the oldest leaves only 25–53% of the capacity in the youngest. The photosynthetic capacity was estimated to be sufficiently high to ensure refixation of the CO2 released from malate during decarboxylation in the daytime. In line with this, a linear coupling between CAM capacity and photosynthetic capacity was found. Parallel to the change in photosynthetic capacity, an age-related change in total ribulose-bisphosphate carboxylase/oxygenase (rubisco) activity from 732 μmol C g−1 DW h−1 in the youngest leaves to 346 μmol C g−1 DW h−1 in the oldest was observed. In contrast, no significant change in phosphoenolpyruvate carboxylase (PEPcase) activity with leaf age was observed (means ranged between 46 and 156 μmol C g−1 DW h−1). 相似文献
Temporal and spatial variation in CH4 emissions was studied at hummock, Eriophorum lawn, flark and Carex lawn microsites in an oligotrophic pine fen over the growing season using a static chamber method, and CH4 production and oxidation potentials in peat profiles from hummock and flark were determined in laboratory incubation experiments.
Emissions were lowest in the hummocks, and decreased with increasing hummock height, while in the lawns and flarks they increased
with increasing sedge cover. Statistical response functions with water table and peat temperature as independent variables
were calculated in order to reconstruct seasonal CH4 emissions by reference to the time series for peat temperature and water table specific to each microsite type. Mean CH4 emissions in the whole area in the snow-free period of 1993, weighted in terms of the proportions of the microsites, were
1.7 mol CH4 m–2. Potential CH4 production and oxidation rates were very low in the hummocks rising above the groundwater table, but were relatively similar
when expressed per dry weight of peat both in the hummocks and flarks below the water table. The CH4 production potential increased in autumn at both microsites and CH4 oxidation potential seemed to decrease. The decrease in temperature in autumn certainly reduced in situ decomposition processes,
possibly leaving unused substrates in the peat, which would explain the increase in CH4 production potential.
Received: 16 August 1996 / Accepted: 30 November 1996 相似文献
Summary A 22-year-old crop ofPinus contorta on oligotrophic peat was found to contain 85 kg ha–1 more K than adjacent unplanted areas. It is suggested that the forest cover aids in conserving rainfall derived K which in the natural (unplanted) state is lost to drainage. 相似文献