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Post-translational regulation of CND41 protease activity in senescent tobacco leaves 总被引:2,自引:0,他引:2
The degradation of chloroplast proteins is an important occurrence in the mobilization of nutrients from senescing leaves
to reproductive organs during senescence. Recently, we proved that tobacco CND41 protease is involved in Rubisco degradation
and the translocation of nitrogen during senescence. In this study, we show the post-translational regulation of CND41 protease.
Using very specific antibodies that were prepared against CND41-specific peptide (anti-Val 186 to Ser 206), immunoblot analysis
clearly indicated a change in the accumulation and processing of CND41 during the maturation of leaves in whole plants. The
developmental modification of CND41 was also observed in transgenic tobacco with constitutive expression of CND41 under cauliflower
mosaic virus 35S promoter. Further studies of seedlings under senescence induced by combined treatment with nitrogen-starvation
and high sucrose confirmed that the processing of CND41 was important for protease activity and senescence. A possible mechanism
for the regulation of CND41 activity is discussed. 相似文献
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