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1.
Pavel Veprek Jan Jezek Jirí Velek Hatem Tallima Mona Montash Rashika El Ridi 《Journal of peptide science》2004,10(6):350-362
Four monoepitopic MAPs (MAP A, B, C and E) and one bis-diepitopic MAP B-E derived fromthe primary sequence of Schistosoma mansoni glyceraldehyde 3-phosphate dehydrogenase, previously tested in BALB/c mice, were examined for their immunogenicity and protective capacity in C57BL/6 mice. Despite multimerization into MAPs, MAP Aand MAP C were poorly immunogenic. In contrast toBALB/c mice, MAP E was non-immunogenic in C57BL/6 mice. Peptide B in the form of MAP B orbis-diepitopic MAPB-E elicited immune responses in C57BL/6 mice that were associated with a significant decrease in worm burden. The MAPs were prepared by the stepwise solid-phase peptide synthesis using Boc/Bzl chemistry, successfully purified on the RP-HPLC column and characterized by RP-HPLC, HPCE and MALDI-TOF MS techniques. A general strategy for MAPs purification is discussed here and the purification of MAP Band MAP E is documented in detail. 相似文献
2.
Summary Adult mice were found to show regional variation in the epithelial expression of some molecules of the blood-group antigen series. To investigate connective tissue influences on such differences, heterotypic recombinants of epithelia and connective tissues from various regions were prepared and examined using monoclonal antibodies directed against bloodgroup antigens H and Ley. The results indicate that epithelia may maintain a preexisting regionally specific pattern following recombination but that, in some recombinant matches, the connective tissue is capable of signalling redirection of the pattern of expression towards that typical of the epithelium with which it is normally associated.This work was supported by NIH-NIDR RO1-DEO-5190 相似文献
3.
An immunohistochemical study on the postnatal development of rat nasal-associated lymphoid tissue (NALT) 总被引:3,自引:0,他引:3
Dona M. H. Hameleers Marja van der Ende Jeike Biewenga Taede Sminia 《Cell and tissue research》1989,256(2):431-438
Summary This study concerns the development of nasal-associated lymphoid tissue in the rat, using immuno- and enzyme-histochemical staining techniques on cryostat sections. Nasal-associated lymphoid tissue is present at birth as a small accumulation of mainly T lymphocytes and non-lymphoid cells; B cells are rare. Distinct areas of T and B cells appear at 10 days after birth; by that time high endothelial venules are also observed. Intra-epithelial lymphocytes are present, most of them being T-helper cells. ED1+ macrophages are seen throughout the tissue. The proportion of ED1+cells does not change during ontogeny. ED2+cells (tissue macrophages) are present predominantly at the border between the lymphoid tissue and the surrounding connective tissue, in all age-groups. ED3+mononuclear cells are scattered throughout the nasal-associated lymphoid tissue of young animals. Later on, the ED3+ cells migrate into the border-area between lymphoid and connective tissue. Ia+ non-lymphoid cells in the nasal lymphoid tissue increase in number during ontogeny. Only a few of them show acid phosphatase activity, indicating that the proportion of classical scavenger macrophages is low. Some of them may be antigen presenting (dendritic) cells. Ia+ dendritic cells also occur between the epithelial cells. Moreover, some epithelial cells express the Ia marker. 相似文献
4.
观察了初生、幼仔、亚成体和老年大熊猫的食管、胃和肠的石蜡切片。与成体的对比,生长发育阶段的主要变化发生在两个主要机能层:粘膜层和肌肉层;衰老的变化则主要出现在粘膜层,尤其是上皮组织的变化最显著,内分泌亲银细胞的数量在生长期似略有增多,成年到老年数量变化不明显。 相似文献
5.
伤寒Vi多糖菌苗接种反应观察 总被引:3,自引:1,他引:2
本文报告了对我国首次成功的伤寒Vi多糖菌苗进行人体接种反应观察结果,接种对象为20至54岁无伤寒病史,近年无伤寒菌苗接种史的健康人,共60名,以完全随机的方法分为两组,实验组注射30μgVi多糖菌苗,对照组注射Vi多糖菌苗的稀释液。其结果30名Vi多糖菌苗接种者注射后体温无中重反应发生,局部红肿仅有1例中反应。注射后对血压、心律没有影响。红细胞计数,白细胞计数均在正常范围,与接种前相比,无显著差异 相似文献
6.
对79名HBV标志阴性少年人群,以乙肝疫苗10μg×3的免疫剂量和0、1、2月的免疫程序进行接种,对其抗-HBs免疫应答和临床保护效果作了为期7年的定人随访。结果表明,抗-HBs阳转率在免后三个月时为100%,均值为3084MIU/ml。至免后84个月时疫苗接受者中仍有55.7%的抗-HBs水平≥10MIU/ml。6例检出抗-HBs,其中5例的抗-HBs持续处于高水平。全部观察对象无一例检出HBsAg或发生临床肝炎。少年接种乙肝血源疫苗至少7年内可具有保护性抗体。故在此期内不需加强免疫。 相似文献
7.
不同月龄婴儿接种DTP后血清中百日咳抗体水平的观测 总被引:2,自引:1,他引:1
本文对比观察了213例2月龄、3月龄婴儿接种DTP后百日咳抗体水平的变化,探讨了母传抗体对免后抗体增长的影响。结果表明2月龄、3月龄婴儿DTP免疫后1个月和3个月血清中百日咳抗体达保护水平的百分率无显著性差异(x ̄2=0.036,p>0.9;x ̄2=0.327,p>0.5),免后1个月抗体GMT无显著性差异(t=0.17,p>0.5),免后3个月抗体GMT3月龄组高于2月龄组(t=2.22,p<0.05)。我们还发现免前抗体水平与免后1个月GMT虽有负相关(r=—0,754)的倾向,但总体上对抑制免后抗体应答不明显,因而建议将儿童DTP基础免疫的起始月龄提前至2月龄进行。 相似文献
8.
Bahman Tabaraie Bal Krishan Sharma Praveen Rishi nee Sharma Rakesh Sehgal Nirmal Kumar Ganguly 《Microbiology and immunology》1994,38(7):553-559
Porins were prepared from smooth strain of Salmonella typhi 0–901 and chemotype of rough mutant of S. typhimurium Ra-30. Mice were immunized with both the porin preparations in different groups and challenged with S. typhimurium LT2–71 and S. enteritidis SH-1269. Porin immunized mice showed significant protection (P <0.01) against challenge with homologous as well as heterologous strains. Hence, the use of porins may be attempted in future to protect against salmonellosis. 相似文献
9.
Per Putkonen Charlotta Nilsson Kerstin Hild Reinhold Benthin Martin Cranage Anne Marie Aubertin Gunnel Biberfeld 《Journal of medical primatology》1993,22(2-3):100-103
Several groups have reported protection against experimental SIV infection in macaques immunized with a whole inactivated virus vaccine. The aim of the current study was to investigate whether five macaques vaccinated with whole inactivated SIV and previously shown to be protected against challenge with two divergent strains of SIV grown on human cells could resist challenge with a subsequent homologous SIV grown on macaque cells. We show here that this same vaccine did not protect when the challenge virus was grown on primary cells of monkey origin. 相似文献
10.
本文对吸附精制百白破混合制剂的不同配方进行了实验,结果表明,新一代吸附精制百白破混合制剂最佳配方为:精制百日咳菌苗18μgPN/ml、精制白喉类毒素为30Lf/ml、精制破伤风类毒素为10Lf/ml。由该配方组成的吸附精制百白破混合制剂,其中百日咳菌苗的毒性试验BWDU/ml、LPU/ml、HSU/ml三种指标均符合制检规程要求。其效力单位(IU/ml)超过规程要求一倍以上,精制白喉和破伤风类毒素的安全试验均符合规程要求,白类效力试验≥80-100%,破类效力试验≥0.5-4.5IU/ml。上述结果说明本文提出的配方作为新一代精制百白破混合制剂的配方是适宜和实用的。 相似文献