Plasma N-glycome composition associates with chronic low back pain

Background

Low back pain (LBP) is the symptom of a group of syndromes with heterogeneous underlying mechanisms and molecular pathologies, making treatment selection and patient prognosis very challenging. Moreover, symptoms and prognosis of LBP are influenced by age, gender, occupation, habits, and psychological factors. LBP may be characterized by an underlying inflammatory process. Previous studies indicated a connection between inflammatory response and total plasma N-glycosylation. We wanted to identify potential changes in total plasma N-glycosylation pattern connected with chronic low back pain (CLBP), which could give an insight into the pathogenic mechanisms of the disease.

Measurement analysis of two radials with a common‐origin point and its application

In spectral analysis, a chemical component is usually identified by its characteristic spectra, especially the peaks. If two components have overlapping spectral peaks, they are generally considered to be indiscriminate in current analytical chemistry textbooks and related literature. However, if the intensities of the overlapping major spectral peaks are additive, and have different rates of change with respect to variations in the concentration of the individual components, a simple method, named the ‘common‐origin ray’, for the simultaneous determination of two components can be established. Several case studies highlighting its applications are presented.     

S_(180)肉瘤细胞对小鼠基因组DNA结构变化影响的核磁共振氢谱~1H—NMR研究

本实验利用核磁共振技术,对体内培养了腹水癌小鼠肝细胞基因组DNA的结构变化进行了测试,发现随致癌时间的延长,基因组DNA内胸腺嘧啶核苷酸周围的化学环境发生了定向的变化,造成DNA核磁共振氢谱中,部分胸腺嘧啶的甲基质子发生了化学位移,在′H-NMR图谱中1.9ppm峰Ⅰ减小,而2.6-2.7ppm区域的峰Ⅱ却明显增加,结果使双峰比(峰Ⅰ积分面积/峰Ⅱ积分面积)显著下降,本文为研究癌症发生和发展的遗传学机制提供了一个新的方法.     

Ecology of rotifers of Surinsar,a subtropical,fresh water lake in Jamme (J & K), India

17 species of rotifers have been recorded from Lake Surinsar, Jammu (J & K), India of which some are either exclusively limnetic (Brachionus angularis, Hexarthra sp., Filinia opliensis), or littoral (Brachionus patulus, M. ventralis, Trichotoria sp., Platyias quadricornis, Lecane (Monostyla) decipiens, L (M), bulla and Lecane sp.) and others (Keratella tropica, Anuraeopsis fissa, Brachionus quadridentatus, B. calyciflorus, Trichocera sp., T. similis, and Polyarthra sp.) seem to be wandering species. Seasonal maxima for both littoral and limnetic zones are reported. Most population maxima are contributed mainly by one or at best two species.Physico-chemical factors like temperature, pH, dissolved oxygen, free carbon dioxide, calcium, magnesium, and total alkalinity have been studied and their infuence on these rotifer species are discussed. On their thermal responses, the available rotifer species have been classified as warm stenothermal, cold stenothermal or eurythermal. The importance of Mytilina ventralis as a biological indicator for dissolved oxygen in this lake has been pointed out.     

Phase determination from direct sequencing of length‐variable DNA regions

For diploid organisms, haplotype determination usually requires sequencing cloned polymerase chain reaction (PCR) products or comparing the genotypes of several individuals. We found out that phase could be reconstructed from direct sequencing of mixed PCR products by combining for each individual the complementary information contained in its forward and reverse chromatograms, provided these products had different lengths. When applied to the internal transcribed spacer 2 (ITS2) from corals of the genus Pocillopora, this new method allowed us to identify two dominant sequence types in some specimens; however, sequencing cloned PCR products from the same specimens yielded more variants, including possible PCR‐generated recombination artifacts.     

Atomic-scale structure of nanocrystals by the atomic pair distribution function technique

The approach of the atomic pair distribution function (PDF) technique to determine the atomic-scale structure of nanocrystalline materials is introduced and illustrated with results of studies on V₂O₅ nanotubes and clusters of Cs atoms intercalated inside the pores of the zeolite ITQ-4. We find that V₂O₅ nanotubes are built of double layers of V-O₅ and V-O₄ units. Inside the channels in ITQ-4 Cs atoms are found to assemble in short-range ordered zigzag chains.     

中国的主要山脉和山峰

基于我国的地貌特征、数字高程模型(DEM)以及数字地形模型(DTM)等资料,参考以往的研究文献,给出了我国主要山峰和山脉的分布图及相关的地理信息,为我国的宏观生态学和生物地理学研究提供基本的山地资料。     

利用基因组学和MALDI-TOF MS技术鉴定放线菌纲细菌的核糖体蛋白质标志物

【目的】基质辅助激光解吸电离飞行时间质谱(MALDI-TOF MS)法基于微生物的特征蛋白指纹图谱鉴定菌种,本研究利用基因组学和MALDI-TOFMS技术鉴定放线菌纲细菌的核糖体蛋白质标志物。【方法】从MALDI-TOF MS图谱数据库选取放线菌纲代表菌种,在基因组数据库检索目标菌种,获取目标菌株或其参比菌株的核糖体蛋白质序列,计算获得分子质量理论值,用于注释目标菌株MALDI-TOFMS指纹图谱中的核糖体蛋白质信号。【结果】从8目,24科,53属,114种,142株放线菌的MALDI-TOFMS图谱中总共注释出31种核糖体蛋白质。各菌株的指纹图谱中核糖体蛋白质信号数量差异显著。各种核糖体蛋白质信号的注释次数差异显著。总共15种核糖体蛋白质在超过半数图谱中得到注释,注释次数最高的是核糖体大亚基蛋白质L36。【结论】本研究找到了放线菌纲细菌MALDI-TOF MS图谱中常见的15种核糖体蛋白质信号,可为通过识别核糖体蛋白质的质谱特征峰鉴定放线菌的方法建立提供依据。     

PEAK SHIFTS PRODUCED BY CORRELATED RESPONSE TO SELECTION

Traits may evolve both as a consequence of direct selection and also as a correlated response to selection on other traits. While correlated response may be important for both the production of evolutionary novelty and in the build-up of complex characters, its potential role in peak shifts has been neglected empirically and theoretically. We use a quantitative genetic model to investigate the conditions under which a character, Y, which has two alternative optima, can be dragged from one optimum to the other as a correlated response to selection on a second character, X. High genetic correlations between the two characters make the transition, or peak shift, easier, as does weak selection tending to restore Y to the optimum from which it is being dragged. When selection on Y is very weak, the conditions for a peak shift depend only on the location of the new optimum for X and are independent of the strength of selection moving it there. Thus, if the “adaptive valley” for Y is very shallow, little reduction in mean fitness is needed to produce a shift. If the selection acts strongly to keep Y at its current optimum, very intense directional selection on X, associated with a dramatic drop in mean fitness, is required for a peak shift. When strong selection is required, the conditions for peak shifts driven by correlated response might occur rarely, but still with sufficient frequency on a geological timescale to be evolutionarily important.