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排序方式: 共有88条查询结果,搜索用时 15 毫秒
1.
Jürgen M. Schmitt Christine Michalowski Hans J. Bohnert 《Photosynthesis research》1988,17(1-2):159-171
Mesembryanthemum crystallinum responds to high salinity in the soil by shifting the mode of carbon assimilation from the C3 mode to Crassulacean acid metabolism (CAM). Several enzymes of carbon metabolism have increased apparent activities in the CAM mode, including phosphoenolpyruvate carboxylase (PEPcase) and pyruvate orthophosphate dikinase (PPDK). We have identified cDNA clones for PEPcase and PPDK by immunological screening of a cDNA library constructed in the protein expression vector lambda gt11. The clones were characterized by immunoblotting and RNA blotting techniques. RNA blotting showed that during CAM induction the steady-state level of mRNAs for both PEP case and PPDK increased.Abbreviations IPTG
isopropyl thiogalactoside
- PEP
phosphoenolpyruvate
- PEPcase
phosphoenolpyruvate carboxylase
- PPDK
pyruvate orthophosphate dikinase
- Xgal-5
bromo-4-chloro-3-indolyl-beta-D-galactopyranoside 相似文献
2.
CAM植物按其对环境的反应可分为两种类型:即专一CAM植物和兼性CAM植物。前者不易受外界环境的变化而改变其CAM性质;后者的光合型可随季节和水分胁迫等而发生变化,也可因人工诱导作用而使其由C_3型转变为CAM型。 相似文献
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Christiane Sommer Barbara Thonke Marianne Popp 《Plant biology (Stuttgart, Germany)》1990,103(3):270-273
Pinitol (1d -3-O-methyl-chiro-inositol) and 1d -1-O-methyl-muco-inositol, two cyclitols wide-spread in the plant kingdom, were isolated from plant sources in order to test their compatibility with malate dehydrogenase activity. Both compounds had no inhibitory effect on malate dehydrogenase from Rhizophora mangle in a range of 100 to 1000 mol . m?3. Their influence on malate dehydrogenase activity from different plant sources (Rh. mangle L., Mesembryanthemum crystallinum L., Cicer arietinum L. and Spinacia oleracea L.) was also small and similar to that observed for a number of well established compatible solutes (e.g. proline, glycine betaine). A possible role of cyclitols as cryoprotectants or radical scavengers is discussed. 相似文献
7.
The structure of the vacuolar ATPase from mesophyll tonoplasts of Mesembryanthemum crystallinum has been studied by electron microscopy using negatively stained specimens of membrane-bound and detergent-solubilized ATPase molecules. We observed a high density of particles on the surface of tonoplast vesicles and “head and stalk” structures on the edge of the membrane, similar to the F0F1-ATPases of mitochondrial and chloroplast membranes. The staining conditions, which are often critical for such small objects, were improved by using methylamine tungstate as negative stain for the membrane-bound ATPase. Compared to other staining solutions generally applied, dissociation of the F1-like enzyme complex from the membrane was best prevented and structural damage of the vesicles was least observed with methylamine tungstate. In freeze-fracture electron microscopy of tonoplast vesicles, where dissociation never occurs since no detergent is used, we also observed “head and stalk” structures on the edge of the membranes, beside many particles on the fracture faces. The detergent-solubilized ATPase forms string-like structures, caused by the aggregation of the hydrophobic membrane-embedded F0-like part of the enzyme. After negative staining the F1-like enzyme complex is arranged alternately along both sides of the string and connected by a narrow stalk. 相似文献
8.
【目的】深入了解针垫花(Leucospermum cordifolium)的开花结实特性,探求其结实率低的原因,推动其种子生产、杂交育种、花期调控以及新品种培育工作。【方法】本文以针垫花(Leucospermum cordifolium)为主要材料,对其开花物候、花部特征、花粉活力、柱头可授性、杂交指数、授粉结实特性进行研究。【结果】1)针垫花的花期一般在冬末、早春至夏季;2)最适合测定其花粉活力的离体萌发培养基为30g/L蔗糖+150mg/L硼酸+50mg/L氯化钙,花粉活力为67.47%;3)在开花第1-7d柱头可授性逐渐加强,第5-7d的时最为强烈;杂交指数估算结果表明针垫花部分自交亲和,异交,需要传粉者;4)田间观测发现其在自然状态下结实率较低,但可以自发完成授粉,不存在无融合生殖现象;5)人工授粉试验发现针垫花异株异花授粉的结实率最高,为17.14%,而自花授粉的结实率最低,为4.94%。【结论】针垫花的雌雄蕊异熟是导致其自然结实率低的原因之一,而雌蕊花柱中及子房部位的胼胝质、花粉萌发后花粉管顶端膨大畸形是造成其结实率低的重要原因。 相似文献
9.
Using the patch-clamp technique, we observed profound oscillations of the whole-vacuole outward current across the tonoplast
of Mesembryanthemum crystallinum L. (common ice plant). These current oscillations showed a clear voltage dependence and appeared at membrane potentials more
positive than 90–100 mV. This paper describes the oscillations in terms of two separate mechanisms. First, the Mesembryanthemum vacuolar membrane shows a negative slope conductance at membrane potentials more positive than 100–120 mV. The fact that
the oscillations and the negative slope conductance show a similar threshold potential suggests that (part of) the same mechanism
is involved in both phenomena. The second mechanism involved is the voltage drop across the series resistance. As a result,
the potential actually experienced by the vacuolar membrane deviates from the command potential defined by the patch-clamp
amplifier. This deviation depends in an Ohmic manner on the current magnitude. We suggest that the interplay of the negative
slope conductance and the voltage drop across the series resistance can cause a positive feedback which is responsible for
the current oscillations.
Received: 30 April 1999/Revised: 9 September 相似文献
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