Molecular localization of two cattle allotypic specificities.

McA1 and McA2 are two cattle allotypic specificities carried on high molecular weight glycoproteins and controlled by allelic genes. Results derived from two independent experimental approaches (digestion of McA1 and McA2 antigen preparations with specific glycosidases and inhibition tests of anti McA1 and anti McA2 with simple sugars) showed that mannose is essential in determining the serological specificity of McA1 and glucose of McA2.     

Endoplasmic reticulum-localized hepatic lipase decreases triacylglycerol storage and VLDL secretion

Hepatic triacylglycerol levels are governed through synthesis, degradation and export of this lipid. Here we demonstrate that enforced expression of hepatic lipase in the endoplasmic reticulum in McArdle RH7777 hepatocytes resulted in a significant decrease in the incorporation of fatty acids into cellular triacylglycerol and cholesteryl ester accompanied by attenuation of secretion of apolipoprotein B-containing lipoproteins. Hepatic lipase-mediated depletion of intracellular lipid storage increased the expression of peroxisome proliferator-activated receptor α and its target genes and augmented oxidation of fatty acids. These data show that 1) hepatic lipase is active in the endoplasmic reticulum and 2) intracellular hepatic lipase modulates cellular lipid metabolism and lipoprotein secretion.