Determination of marbofloxacin in plasma samples by high-performance liquid chromatography using fluorescence detection

A simple and sensitive HPLC method has been developed for the determination of marbofloxacin (MAR) in plasma. Sample preparations were carried out by adding phosphate buffer (pH 7.4, 0.1 M), followed by extraction with trichloromethane. MAR and the internal standard, enrofloxacin (ENR), were separated on a reversed-phase column and eluted with aqueous solution–acetonitrile (80:20). The fluorescence of the column effluent was monitored at λ_ex=338 and λ_em=425 nm. The retention times were 2.20 and 3.30 min for MAR and ENR, respectively. The method was shown to be linear from 15 to 1500 ng/ml (r²=0.999). The detection limit was 15 ng/ml. Mean recovery was determined as 90% by the analysis of plasma standards containing 150, 750, and 1500 ng/ml. Inter- and intra-assay precisions were 3.3% and 2.7%, respectively.     

Efficacy of marbofloxacin for naturally occurring contagious caprine pleuropneumonia

The aim of the present study is to determine the efficacy of marbofloxacin used in goats with naturally occurring contagious caprine pleuropneumonia (CCPP). The study was performed in two groups (consisting of 15 animals in each group) with two different doses of 10% aqueous solution of marbofloxacin injected intramuscularly into the semitendinous muscle. 2 mg/kg BW for 3 days (total dose administered: 6 mg/kg BW) was injected to the first group (group 1) and 3 mg/kg for 2 times every other day (total dose administered: 6 mg/kg BW) was injected to the second group (group 2). Microbiological analyses revealed that the causative agent of the disease was Mycoplasma capricolum subsp. capripneumoniae. Cure rates for groups 1 and 2 were determined as 100% (15/15 goats) and 93% (14/15 goats), respectively. The results of this field trial suggest that marbofloxacin could be an effective drug against CCPP in goats.