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HPLC法测定山茱萸注射液中马钱素和莫诺甙的含量 总被引:8,自引:0,他引:8
山茱萸注射液中有多种有效成分,采用高效液相色谱法对山茱萸注射液中的马钱素(Loganin)和莫诺甙(Morroniside)进行分离定量.色谱柱:Kromasil-100℃_(18)柱.流动相:0.05mol/L磷酸二氢钠缓冲液-乙腈(6.4:1).马钱素和莫诺甙的平均回收率和相对标准偏差分别为99.79%、1.51(n=6)和99.73%、2.75%(n=6).结果满意,可作为山茱萸主射液质量控制的标准方法. 相似文献
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The Madagascar periwinkle (Catharanthus roseus) produces numerous indole alkaloids, several of which have an important pharmaceutical uses such as ajmalicine, vinblastine
and vincristine. The relationship between hypoxia and ajmalicine production in a cell suspension culture of C. roseus were investigated during the cycle of cell culture, in correlation with the effects on growth. The results show that the
lack of oxygenation in C20D cells provokes a very strong inhibition in accumulation of the alkaloids and of other possible
substances. Moreover, the addition of loganin, a metabolic intermediate of the biosynthetic pathway, in the culture medium
of cells subjected to hypoxia restored the alkaloid production. Also, the results showed that the addition of benzyladenine
(BA) to the culture medium increased the ajmalicine production and that the inhibitory effect of hypoxia was almost absent
in these conditions. Therefore, it could be suggested that BA can without doubt decrease the effects of the hypoxia and increase
the ajmalicine production in periwinkle cell suspensions. 相似文献
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Cornus officinalis is a small deciduous tree with significant medicinal values. To explore the ecological effects on phenotypic, cytological and biochemical diversity of medicinal plants, we estimated the loganin content, C-values and fruit and seed phenotypic traits of 80 C. officinalis germplasms collected from across three genuine producing areas of China and Madison, USA. Our results showed that most fruit and seed phenotypic traits of C. officinalis germplasms from Madison, USA were significantly larger than those from China, but the loganin contents and 2C values of germplasms from China (0.9% and 5.92 pg) were significantly higher than those from Madison (0.6% and 5.29 pg) (P < 0.01). These data highlight the fact that there was a tight association between temperature, precipitation and loganin content in C. officinalis. Based on the above results, we found that C. officinalis had a tendency to decline in genome size (GS) and loganin content during the long period of adaptation and evolution with new ecological variables. We are able to presume that the ecological variable is the key factor in determining phenotypic diversity, GS and active ingredient of medicinal plants. 相似文献
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Jasmonates enhance the expression of various genes involved in terpenoid indole alkaloid (TIA) biosynthesis in Catharanthus roseus. We applied precursor feeding to our C. roseus suspensions to determine how methyl jasmonate (MJ) alters the precursor availability for TIA biosynthesis. C. roseus suspensions were induced with MJ (100 μM) on day 6 and fed loganin (0.30 mM), tryptamine (0.15 mM), loganin plus tryptamine, or geraniol (0.1–1.0 mM) on day 7. While MJ increased ajmalicine production by 3-fold, induced cultures were still limited by terpenoid precursors. However, both induced and non-induced cultures became tryptamine-limited with excess loganin. Geraniol feeding also increased ajmalicine production in non-induced cultures. But MJ appeared to increase geraniol availability in induced cultures, due presumably to the increased expression of Dxs with MJ addition. 相似文献
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