Anticancer effects of melatonin via regulating lncRNA JPX-Wnt/β-catenin signalling pathway in human osteosarcoma cells

Osteosarcoma (OS) is a type of malignant primary bone cancer, which is highly aggressive and occurs more commonly in children and adolescents. Thus, novel potential drugs and therapeutic methods are urgently needed. In the present study, we aimed to elucidate the effects and mechanism of melatonin on OS cells to provide a potential treatment strategy for OS. The cell survival rate, cell viability, proliferation, migration, invasion and metastasis were examined by trypan blue assay, MTT, colony formation, wound healing, transwell invasion and attachment/detachment assay, respectively. The expression of relevant lncRNAs in OS cells was determined by real-time qPCR analysis. The functional roles of lncRNA JPX in OS cells were further examined by gain and loss of function assays. The protein expression was measured by western blot assay. Melatonin inhibited the cell viability, proliferation, migration, invasion and metastasis of OS cells (Saos-2, MG63 and U2OS) in a dose-dependent manner. Melatonin treatment significantly downregulated the expression of lncRNA JPX in Saos-2, MG63 and U2OS cells. Overexpression of lncRNA JPX into OS cell lines elevated the cell viability and proliferation, which was accompanied by the increased metastasis. We also found that melatonin inhibited the OS progression by suppressing the expression of lncRNA JPX via regulating the Wnt/β-catenin pathway. Our results suggested that melatonin inhibited the biological functions of OS cells by repressing the expression of lncRNA JPX through regulating the Wnt/β-catenin signalling pathway, which indicated that melatonin might be applied as a potentially useful and effective natural agent in the treatment of OS.     

LncRNA AL133467.1作为miR-661的ceRNA抑制乳腺癌细胞增殖和侵袭

长非编码RNAs((long non-coding RNAs,lncRNAs)在多种肿瘤中异常表达并参与肿瘤的发生发展.然而,众多lncRNAs在肿瘤的表达及功能尚未完全阐明.本文通过分析TCGT数据库113例正常乳腺组织和1109例乳腺癌组织发现,LneRNA AL133467.1在乳腺癌组织中低表达,并与乳腺癌患者...     

A novel lncRNA,Lnc-OC1, promotes ovarian cancer cell proliferation and migration by sponging miR-34a and miR-34c

Long non-coding RNAs (lncRNAs) have been reported to be of great importance in tumorigenesis and progression of a variety of cancers. However, the role of lncRNAs in ovarian cancer (OC) remains largely unknown. In the present study, we identified a novel lncRNA, LOC100288181 (named as Lnc-OC1), which acted as a key regulator in the development and progression of OC. The combined Gene Expression Omnibus (GEO) database analysis revealed that Lnc-OC1 was significantly upregulated in OC tissues and Kaplan-Meier survival analysis confirmed that high Lnc-OC1 expression was associated with poor prognosis of OC patients. Importantly, we also demonstrated that knockdown of Lnc-OC1 suppressed cell proliferation, colony formation, invasion and migration in vitro and inhibited tumorigenicity in vivo. Mechanistically, Lnc-OC1 repressed the expression of endogenous miR-34a and miR-34c as a sponge and vice versa. Moreover, rescue experiments demonstrated that the oncogenic function of Lnc-OC1 at least partially depended on suppressing miR-34a and miR-34c. In conclusion, our results suggest that the Lnc-OC1-miR-34a/34c axis may play a pivotal role in OC, and may serve as a potential diagnostic biomarker and a powerful therapeutic target for OC.     

死亡相关的LncRNAs在甲状腺癌中的预后价值及预后风险模型构建

目的:探讨铁死亡相关的长链非编码RNAs(LncRNAs)在甲状腺癌中的预后价值并构建预后风险模型。方法:从癌症基因组图谱(TCGA)数据库下载甲状腺癌的转录本数据和临床数据,铁死亡相关的基因数据集是从铁死亡数据库(http://www.zhounan.org/ferrdb/)下载的259个基因集。得到铁死亡相关LncRNAs,与患者临床信息合并后,通过单因素Cox回归分析和Kaplan-Meier生存分析两种方法得到与甲状腺癌预后相关的铁死亡LncRNAs,通过R的survival包构建COX模型以此来建立最佳预后风险模型并予以验证。结果:获得30个铁死亡相关的LncRNAs,多因素cox分析得到10个与甲状腺癌预后相关的铁死亡LncRNAs,包括AL136366.1、AL162231.2、CRNDE、AC004918.3、LINC02471、AC092279.1、AC046143.1、LINC02454、DOCK9-DT、AC008063.1。Kaplan-Meier生存分析表明高风险组预后较差。单因素和多因素Cox分析表明风险评分可以作为独立预后因子。KEGG通路富集分析发现,差异基因可能与嘧啶代谢、核苷酸切除修复、NOTCH_信号通路等通路有关。结论:通过生物信息学方法筛选出10个与甲状腺癌预后的铁死亡相关LncRNAs,并成功构建预后风险模型。     

长链非编码RNA在胃癌中的研究进展

长链非编码RNA(1ong non-coding RNA,lnc RNA)是一组长度超过200 bp、缺少特异开放阅读框、不具备完整蛋白编码功能的RNA,其在表观遗传学调控以及转录和转录后调控等方面发挥重要作用。目前,在乳腺癌和肝癌中对Lnc RNA的研究颇多,而对胃癌中Lnc RNA的报道却刚刚兴起。近几年越来越多的研究发现,在胃癌中有很多特异表达的Lnc RNA与胃癌的发生、发展、侵袭、转移密切相关。本文结合国内外最新研究就lnc RNA在胃癌中的研究进展作一简要综述,主要介绍了Lnc RNA在肿瘤研究中的最新发现,尤其是其与胃癌发生发展的密切联系,旨在为胃癌的诊断和治疗提供新思路。