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排序方式: 共有113条查询结果,搜索用时 31 毫秒
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Cell suspension cultures of Catharanthus roseus G. Don, Glycine max (L.) Merr. and Nicotiana tabacum L. were incubated with [14C]acetate, [14C]oleic acid and [14C]linoleic acid at five different temperatures ranging from 15 to 35° C. When the incubation temperature was increased, [14C]acetate was incorporated preferentially into [14C]palmitate, with a concomitant drop in [14C]oleate formation. Between 15 and 20° C, [14C]oleic acid accumulated in C. roseus cells. In all cultures, optimum desaturation of [14C]oleic acid to [14C]linoleic acid occurred between 20 and 25° C, and in G. max this was also the optimal range for desaturation of [14C]linoleic acid to [14C]linolenic acid. Elongation of [14C]palmitic acid was inhibited when cultures grown at 15° C for 25 h were subsequently incubated with [14C]acetate at 25° C. [14C]oleic acid accumulated in G. max and C. roseus cultures grown at 35° C for 25 h and subsequently incubated at 25° C. Desaturation of [14C]oleic acid increased up to 25° C, but then decreased or leveled off depending on the cell line and on the temperature prior to incubation. 相似文献
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Sivaraman Indira Arumugam Neelakantan Sodhi Yashpal Singh Gupta Vibha Mukhopadhyay Arundhati Pradhan Akshay K. Burma Pradeep Kumar Pental Deepak 《Molecular breeding : new strategies in plant improvement》2004,13(4):365-375
A zero erucic acid (C22:1) line of Brassica juncea (VH486), adapted to the agronomic conditions of Northern India, has been modified for its fatty acid composition in the seed oil with antisense constructs using the sequence of fad2 gene of B. rapa. The full-length B. rapa fad2 cDNA sequence was determined by 5 and 3 RACE of a partial sequence available in the EST database. Construct pASfad2.1 contained 315 to 1251 bp and construct pASfad2.2 contained 1 to 1251 bp fragment of the fad2 gene, both in antisense orientation, driven by a truncated napin promoter. Analysis of the levels of linoleic acid (C18:2) in the BC1 seeds of single-copy transgenics showed that the construct pASfad2.2 gave better suppression of the fad2 gene as compared to the construct pASfad2.1. The BC1 transgenic seeds containing the pASfad2.2 construct segregated into two distinct classes of C18:2>20% (putative null homozygotes) and C18:2<20% (putative heterozygotes) in a 1:1 ratio, while the T1 seeds segregated into three classes, C18:2>20%, C18:2 between 12% and 20%) and C18:2<12% (putative homozygotes) in a 1:2:1 ratio. Putative homozygous T1 seeds (C18:2<12% analyzed by the half-seed method) of four of the transgenic lines were grown to establish T2 homozygous lines. These had ca. 73% C18:1 and 8 to 9% each of C18:2 and C18:3 (-linolenic acid) fractions in comparison to ca. 53% C18:1, 24% C18:2 and 16% C18:3 in the parental line VH486. 相似文献
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López Y. Nadaf H.L. Smith O.D. Simpson C.E. Fritz A.K. 《Molecular breeding : new strategies in plant improvement》2002,9(3):183-192
Increasing the oleic to linoleic acid ratio (O/L) in peanut has positiveeffects on peanut quality and its nutritional value. 12-Fattyacid desaturases (12-Fad) have been targeted as logicalcandidates controlling the high oleate trait. A previous study using genomicDNA identified an insertion and a polymorphism resulting in an amino acid changeassociated with the high oleate trait in Spanish-type peanut cultivars. Theobjectives of this research were to use RT-PCR to confirm that the SingleNucleotide Polymorphims (SNPs) identified by analysis of genomic DNA wereexpressed, and to determine if expression patterns for 12-Fadwere the same in both seeds and leaves. A polymorphic region of the12-Fad containing a series of nucleotide changes wasamplified, cloned, and sequenced from mRNA of 155 clones of two parental linesand their independent derived backcross lines (IDBLs). The latter differed intheir oleic to linoleic ratio. Data indicated that the Ainsertion and the amino acid change were expressed in both leaf and seed tissue of thehigh and low-intermediate O/L genotypes. It is postulated that several copiesof the 12-Fad are present in the genome. It is reasonable toconclude that total activity, and ultimately the O/L ratio, is dependent on thenumber of functional copies. The results provide the basis for an assay toscreen for the high O/L ratio at the molecular level. We also report thepresence of another isozyme of 12-Fad with high homology tosoybean isozyme 2 that was expressed in seeds.
These authors contributed equally to this work 相似文献
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Iwahashi H 《Free radical research》2003,37(9):939-945
ESR, HPLC-ESR and HPLC-ESR-MS analyses were performed for the reaction mixtures of oxidized linoleic acid with ferrous ions combined use of spin trapping technique. More than 14 peaks were detected on the HPLC-ESR elution profile. In addition to 7-carboxyheptyl and pentyl radicals, several new radicals such as 7-carboxy1dihydroxyheptyl, 1,5-dihydroxypentyl, 8-carboxy-1-hydroxyoctyl, 7-carboxy-1-hydroxyheptyl, 1-hydroxypentyl and 1-hydroxyhexyl were identified using HPLC-ESR and HPLC-ESR-MS. 相似文献
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Summary. In the literature taurine is characterized as a non-specific growth or blood clotting factor, an antioxidant, a membrane
protector, or a regulator of calcium ion homeostasis, just as vitamins A, D, E, F, and K are similarly characterized. On the
basis of recent finding concerning the relationship between taurine and the aldehyde of vitamin A-retinal (Petrosian and Haroutounian,
1988, 1998; Petrosian et al., 1996), as well as on the basis of data from the literature, we now suggest a hypothesis that
taurine promotes the bioavailability of the lipid soluble vitamins A, D, E, K, and F, probably by forming different types
of water soluble, easily hydrolyzable complexes. It is quite possible that the ability of taurine to convert lipids and lipid
soluble substances into a water soluble state is the key to understanding the unusually wide diversity of biological phenomena
associated with taurine. This form of delivery may be an additional, secondary mechanism for the transport of lipid soluble
vitamins, which was probably acquired early in evolution, and remains extremely important for mammals and humans directly
after birth for a variety of physiological functions such as: vision in normal and in emergency situations, rapid blood clotting,
sperm eruption, and situations requiring a prompt consumption of lipid soluble vitamins characteristic of excitable systems.
Clearly, the role of taurine in the physiology of the water insoluble vitamins remains an enigma and is worthy of further
investigations.
Received December 23, 1999; Accepted December 28, 1999 相似文献
8.
龙生型高油酸花生种质油酸亚油酸含量及其比值的遗传分析 总被引:2,自引:1,他引:1
应用植物数量性状主基因+多基因混合遗传模型,对2个龙生型花生高油酸种质与低油酸珍珠豆型品种杂交组合F2的油酸、亚油酸含量及其比值(O/L值)进行遗传分析,结果表明:花生油酸、亚油酸含量的遗传均表现为1对主基因加性-显性模型。控制油酸含量主基因的加性、显性效应值和遗传率在组合I中分别为8.6281、-2.0164和65.26%,在组合II中则分别为10.6638、1.0652和71.39%;控制亚油酸含量主基因的加性、显性效应值和遗传率在组合I中分别为8.0327、1.2858和73.64%,在组合II中则分别为9.0885、-1.0826和71.59%。O/L值的遗传表现为2对主基因加性-显性-上位性模型。2对主基因的加性效应值分别为0.6855、0.6814(组合I)和1.6842、0.8835(组合II),显性效应值分别为-0.6838、0.024(组合I)和-1.6559、-0.5127(组合II);加性×加性效应(i)、加性×显性效应(jab)、显性×加性效应(jba)、显性×显性效应(l)分别为0.6812、0.024、-0.6803、-0.0244(组合I)和0.8822、-0.5124、-0.8594、0.496(组合II);组合I、II主基因遗传率分别为82.57%和88.64%。 相似文献
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Eight-carbon volatiles in mushrooms and fungi: properties, analysis, and biosynthesis 总被引:1,自引:0,他引:1
Eight-carbon volatiles are ubiquitous among fungi and characteristic of the fungal aroma. They are the product of the oxidation
and cleavage of the fatty acid linoleic acid and are classified as oxylipins, molecules taking part in a wide range of biological
processes. Their involvement in the fungal aroma, interactions with pests and pathogens, and reproductive events are reviewed
here, as well as the enzymic systems involved in their biosynthesis. 相似文献