Tissue culture independent transformation for Corchorus olitorius

An efficient microprogation protocol has been developed for Dendrobium densiflorum Lindl. ex Wall., a traditional medicinal plant, through protocorm-like bodies (PLBs) from nodal stem segments using 6-benzylamino-purine (BAP) and the lanthanoid neodymium. The highest percentage of explants producing PLBs (72%), with an average of 15 PLBs per explant, was induced by culturing stem segments on Murashige and Skoog (MS) medium supplemented with 5.0 mg l⁻¹ BAP. The newly formed PLBs proliferated well on the basal MS medium and completely converted into shoots on MS medium containing 2.0 mg l⁻¹ BAP. Shoots produced an average of 22 roots per plantlet when cultured on MS medium supplemented with 2.0 mg l⁻¹ neodymium nitrate. Healthy plantlets with well-developed roots were successfully acclimatized. The obtained result suggests that the lanthanoids can be used to effectively initiate rooting in the micropropagation and conservation of D. densiflorum.     

Efficient synthetic route to anhydrous mononuclear tris(8-quinolinolato)lanthanoid complexes for organic light-emitting devices

A new lanthanoid 8-quinilinolates type structure was found for lanthanum complex La₃(q^Me)₉(H)(NO₃) (1) formed in the reaction of La(NO₃)₃ · 6H₂O with 2-methyl-8-hydroxyquinoline (Hq^Me) and aqueous ammonia in methanol. The molecule of 1 contains three La atoms connected by six bridging quinolinolate ligands, two terminated η²-coordinated q^Me ligands, one terminated η¹-coordinated q^Me ligand and one terminated NO₃ group. The geometry and ¹H NMR spectrum of the complex suggest that it is bearing −1 charge balanced by proton, which was localized objectively. The arrangement of the compound in crystalline state and in pyridine solution is discussed. Syntheses of water- and acid residual-free mononuclear lanthanoid quinolinolates La(q^Me)₃(py)₂ (2) and Lnq₃(py)₂, (Ln = Y (3), La (4), Sm (5), Eu (6), Tb (7), Er (8), Tm (9); q = 8-quinolinolate, py = pyridine) by the reaction of appropriate amido complexes Ln[N(SMe₃)₂]₃ with 3 equiv. of 2-methyl-8-hydroxyquinoline or 8-hydroxyquinoline in pyridine solution is also described. The complex Laq₃(Ph₃PO)₂ (10) was prepared by treatment of 4 with triphenylphosphine oxide in pyridine solution. Lanthanum 2 complex revealed photoluminescence intensity ca. 3 × 10³ times higher than that of the compound 1 prepared by the traditional way in water-alcohol medium. These data give a ground to consider the Lnq₃(py)₂ complexes as promising material for design of light-emitting devices.