小麦Kr基因在小麦与玉米或鸭茅状摩擦禾杂交中的失活

用３７个小麦（Ｔｒｉｔｉｃｕｍａｅｓｔｉｖｕｍ）品种（系）为母本,分别与黑麦（Ｓｅｃａｌｅｃｅｒｅａｌｅ）、球茎大麦（Ｈｏｒｄｅｕｍｂｕｌｂｏｓｕｍ）、玉米（Ｚｅａｍａｙｓ）和鸭茅状摩擦禾（Ｔｒｉｐｓａｃｕｍｄａｃｔｙｌｏｉｄｅｓ）杂交,比较其亲和性,小麦和玉米或鸭茅状摩擦禾杂交比小麦与黑麦或球茎大麦杂交的亲和性显著提高。携带着显性Ｋｒ１和Ｋｒ２基因的小麦品种Ｈｏｐｅ与黑麦杂交,不能形成胚,而与玉米及鸭茅状摩擦禾杂交时,成胚率分别达１６．００％和３２．５０％。表明控制小麦与黑麦及球茎大麦杂交亲和性的Ｋｒ基因系统在小麦与玉米及小麦与鸭茅状摩擦禾属间杂交中失活。讨论了还存在有其它控制小麦属间杂交亲和性的遗传调控系统的可能性。     

环磷酰胺辅助化疗对不同年龄乳腺癌患者卵巢功能的损伤情况及机制研究

目的:以月经变化情况及血清激素水平为观察指标,探讨环磷酰胺辅助化疗方案对绝经前不同年龄段乳腺癌患者卵巢功能的损伤情况及可能的机制研究.方法:收集2017年1月至2018年12月期间在我院接受环磷酰胺辅助化疗的绝经前乳腺癌患者77例.患者年龄范围为26-48岁,并按年龄分为两组:≤ 35岁组为16例;＞35岁组为61例....     

寄主植物对棉铃虫越冬蛹抗寒能力的影响

通过对滞育蛹过冷却点的测定 ,初步明确寄主对棉铃虫Helicoverpaarmigera的越冬抗寒性有影响 ,幼虫取食棉花时产生的滞育蛹的过冷却点低于取食玉米的滞育蛹的过冷却点 ,即前者的抗寒能力高于后者 ;但用Bt棉喂养后的棉铃虫滞育蛹抗寒能力下降     

Cloning and characterization of Methoprene-tolerant(Met)and Kruppel homolog 1(Kr-h1)genes in the wheat blossom midge,Sitodiplosis mosellana

Juvenile hormone(JH),a growth regulator,inhibits ecdysteroid-induced meta-morphosis and controls insect development and diapause.Methoprene-tolerant(Met)and Krippel homolog I(Kr-h1)are two proteins involved in JH action.To gain some insight into their function in development of Sitodiplosis mosellana,an insect pest undergoing obligatory larval diapause at the mature 3rd instar stage,we cloned full-length complemen-tary DNAs of Met and Kr-h1 from this specics.SmMet encoded a putative protein,which contained three domains typical of the bHLH-PAS family and eight conserved amino acid residues important for JH binding.SmKr-h1 encoded a protein showing high sequence homology to its counterparts in other specics,and contained all eight highly conserved Zn-finger motifs for DNA-binding.Expression patterns of SmMet and SmKr hl were de-velopmentally regulated and JH III responsive as well.Their mRNA abundance increased as larvae entered carly 3rd instar,pre-diapause and maintenance stages,and peaked during post-diapause quiescence,a pattern correlated with JH titers in this species.Different from reduced expression of SmMer,SmKr-h1 mRNA increased at mid-to-late period of post-diapause development.Topical application of JH II on diapausing larvac also induced the two genes in a dose-dependent manner.Expression of SmuMer and SmKr-h1 clearly declined in the pre-pupal phase,and was significantly higher in female adults than male adults.These results suggest that JH-responsive SmMet and SmKr-h1 might play key roles in diapause induction and maintenance as well as in post-diapause quiescence and adult reproduction,whereas metamorphosis from larvae to pupac might be correlated with their reduced expression.     

Very early and transient vegetal-plate expression of SpKrox1, a Krüppel/Krox gene from Strongylocentrotus purpuratus

All endodermal and mesenchymal cells of the sea urchin embryo descend from the vegetal plate, a thickened epithelium of approximately 50 cells arising at the early blastula stage. Cell types that derive from the vegetal plate are specified conditionally by inductive interactions with underlying micromeres, but the molecular details of vegetal-plate specification remain unresolved. In a search for regulatory proteins that have roles in vegetal-plate specification, a screen was performed to clone Krüppel/Krox-related genes from a Strongylocentrotus purpuratus embryo cDNA library. One newly identified clone, named SpKrox1, contained four zinc fingers and a leucine zipper domain. SpKrox1 expression was low in unfertilized eggs, increased severalfold to the early blastula stage and decreased between the early gastrula and pluteus stages. SpKrox1 mRNA was first seen in macromeres of 16-cell stage embryos and was restricted to cells of the developing vegetal plate thereafter. Vegetal-plate expression corresponded to a ring of cells around the blastopore and overlapped the expression patterns of other genes with potential roles in vegetal plate-specification. As the vegetal-plate cells invaginated into the blastopore, SpKrox1 expression was lost, suggesting that its role was not in endoderm differentiation per se but rather in the initial establishment of the vegetal plate.     

慢性阻塞性肺疾病大鼠转录因子KLF2对Nrf2调控γ-GCS表达的影响

目的:研究肺Krüppel样转录因子(KLF2/LKLF)在慢性阻塞性肺疾病(COPD)大鼠肺组织中的表达,探讨KLF2与红系衍生因子2(Nrf2)之间的关系以及对抗氧化酶γ-谷氨酰半胱氨酸合酶(γ-GCS)表达的影响。方法:复制大鼠COPD模型,应用免疫组化、Western blot、原位杂交和RT-PCR方法检测KLF2、Nrf2、γ-GCS蛋白及mRNA的表达,并行相关分析。同时利用免疫共沉淀技术(CO-IP)研究KLF2与Nrf2蛋白之间的相互关系。结果:免疫组化及Western blot结果显示COPD组KLF2、Nrf2、γ-GCS蛋白水平较对照组明显升高(P<0.05);原位杂交及RT-PCR显示KLF2、γ-GCS mRNA水平在COPD组显著高于对照组(P<0.01),而Nrf2 mRNA在两组表达无明显差异(P>0.05);CO-IP结果显示在Nrf2抗体捕获的免疫沉淀中,KLF2抗体可杂交出明显的蛋白条带(P<0.01);直线相关分析发现KLF2蛋白与Nrf2蛋白呈正相关(P<0.05),KLF2、Nrf2蛋白与γ-GCS蛋白及mRNA均呈正相关(均P<0.05)。结论:KLF2在COPD肺组织中表达上调,可能通过活化Nrf2,促进Nrf2核积聚上调γ-GCS的基因表达,在氧化失衡中发挥作用。     

Detection of QTLs for crossability in wheat using a doubled-haploid population

 An intervarietal molecular-marker map was used for the detection of genomic regions influencing crossability between wheat (Triticum aestivum L. em Thell) and rye (Secale cereale L.). Analysis of deviance and logistic marker-regression methods were conducted on data from doubled haploid lines from a cross between “Courtot” and “Chinese Spring”. A major quantitative trait locus (QTL) involved in crossability, associated with the marker Xfba367-5B, was detected on the short arm of chromosome 5B. An additional locus, Xwg583-5B, was indicated on the long arm of chromosome 5B. This minor QTL might correspond to Kr1 which was presumed to be the major gene controlling crossability. Another locus of the genome, Xtam51-7A on chromosome 7A, was significantly associated with this trait. Alleles of “non-crossability” were contributed by the non-crossable cultivar “Courtot”. The three-marker model explains 65% of the difference in crossability between the two parents. The present results are discussed in relation to those previously carried out to locate the Kr genes by using the telocentric mapping technique. Received: 27 February 1998 / Accepted: 15 May 1998