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Genbo Xu Changwei Shao Xiaolin Liao Yongsheng Tian Songlin Chen 《Conservation Genetics》2009,10(4):1009-1011
We describe the first isolation of 12 polymorphic microsatellite markers from Belenger’s jewfish (Johnius belengnerii Cuvier 1830). From a (GT)n-enriched genomic library, 54 microsatellites were selected for designing microsatellite primers,
of which 36 gave working primer pairs. 12 of these loci were polymorphic in a test population of 21 individuals with alleles
ranging from 3 to 18, and expected and observed heterozygosities from 0.5772 to 0.9449 and from 0.4286 to 0.9231, respectively.
No significant linkage disequilibrium between pairs of loci was found, however, loci Jobe24 significantly deviated from Hardy–Weinberg
equilibrium after Bonferroni correction. These polymorphic microsatellite loci should provide sufficient level of genetic
diversity to investigate population structure in Belenger’s jewfish. 相似文献
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Kunio Sasaki 《Ichthyological Research》1999,46(3):271-279
A new sciaenid,Johnius (Johnieops) philippinus, is described from the Davao Gulf, Mindanao Island Philippines. It differs from all known species of the subgenus in the
combination of 29–32 dorsal fin soft rays, 5–6 scales above and 10–13 scales below the lateral line, 10–12 lower gill rakers,
a broadly rounded anterior snout margin (from dorsal aspect), large eyes (28–35% HL), a narrow interorbital space (23–28%
HL) and well-developed pleural ribs on 11th vertebra. A synopsis of species included in the subgenusJohnieops is provided. 相似文献
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Fish bone peptide promotes osteogenic differentiation of MC3T3‐E1 pre‐osteoblasts through upregulation of MAPKs and Smad pathways activated BMP‐2 receptor 下载免费PDF全文
Seung Yun Nam Junghwan Oh Young‐Mog Kim Jae‐Il Kim Namwon Kim Myunggi Yi Won‐Kyo Jung 《Cell biochemistry and function》2018,36(3):137-146
Fish bone, a by‐product of fishery processing, is composed of protein, calcium, and other minerals. The objective of this study was to investigate the effects of a bioactive peptide isolated from the bone of the marine fish, Johnius belengerii, on the osteoblastic differentiation of MC3T3‐E1 pre‐osteoblasts. Post consecutive purification by liquid chromatography, a potent osteogenic peptide, composed of 3 amino acids, Lys‐Ser‐Ala (KSA, MW: 304.17 Da), was identified. The purified peptide promoted cell proliferation, alkaline phosphatase activity, mineral deposition, and expression levels of phenotypic markers of osteoblastic differentiation in MC3T3‐E1 pre‐osteoblast. The purified peptide induced phosphorylation of mitogen‐activated protein kinases, including p38 mitogen‐activated protein kinase, extracellular regulated kinase, and c‐Jun N‐terminal kinase as well as Smads. As attested by molecular modelling study, the purified peptide interacted with the core interface residues in bone morphogenetic protein receptors with high affinity. Thus, the purified peptide could serve as a potential pharmacological substance for controlling bone metabolism. 相似文献
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