Identification of protein bands in polyacrylamide gel by protein printing

Previously described methods for identification of proteins separated in cylindrical polyacrylamide gels have been found to be costly in time and antiserum and difficult to apply to small amounts of protein as are found in cerebrospinal fluid. We describe a method which involves printing of the proteins on the cut surface of the gel onto nitrocellulose paper. The protein bands of the imprint can then be identified using labelled antibodies. We have found this to be economical and quick, and it has permitted sensitive and reliable identification of proteins in unconcentrated cerebrospinal fluid and aqueous humour.     

免疫固定电泳检测对浆细胞肿瘤相关肾损害的诊断价值

目的:探讨免疫固定电泳(immunofixation electrophoresis,IFE)检测对浆细胞肿瘤相关肾损害的诊断价值。方法:回顾性分析我院肾病科665例住院患者的免疫固定电泳分型结果及检出M蛋白带的患者骨髓检查结果。结果:665例肾病科患者中检出44例单克隆免疫球蛋白带,检出率6.6%,其中IgG型24例(κ型6例,λ型18例),IgA型10例(κ型6例,λ型4例),IgM型2例(均为κ型),单纯轻链κ型2例,单纯轻链λ型6例;此44例患者经骨髓检查,38例确诊为多发性骨髓瘤(multiple myeloma,MM),2例确诊为巨球蛋白血症(waldenstrom macroglobulinemia,WM),骨髓涂片中骨髓瘤细胞比例均≥10%;骨髓瘤细胞10%的4例,诊断为意义未明单克隆免疫球蛋白血症(monoclonal gammopathy of un-determined significance,MGUS)。结论:免疫固定电泳检测可对浆细胞肿瘤相关肾损害提供重要的诊断和分型依据,值得临床应用。     

Impairment of Blood-Cerebrospinal Fluid Barrier in Multiple Sclerosis

The blood-CSF barrier (BCB) function in active multiple sclerosis (MS) was studied by means of CSF proteins analysis using disc electrophoresis and immunofixation. Forty-five CSF samples were obtained by repeat lumbar punctures at various intervals, from four autopsy-proven cases and three male and nine female patients with clinically definite MS. When total protein content was increased, the percentages of prealbumin and tau fraction were decreased significantly in association with the presence of haptoglobin (Hp) polymers in nearly all the samples, as a result of increased permeability of the BCB. Even when the total protein content was normal, Hp polymers were detected in 56% of the samples, and the tau fraction tended to be decreased. Monoclonal immunoglobulin and Hp polymers were both recognized in some cases. The results suggested a more frequent occurrence of BCB impairment in MS than had formerly been revealed by CSF albumin analysis, and accorded with the recent reports of contrast-enhancing lesions of MS brain in computerized tomography.     

中国武汉地区汉族人补体第四成份(C4)的遗传多态现象

对神经氨酸酶处理去涎的血浆进行高压琼脂糖电泳后,分别应用免疫固定和溶血覆盖技术调查了我国武汉地区汉族180人的C4多态现象。在C4A座位发现5个变型:C4A4、3、2、1和91;在C4B座位发现6个变型:C4B 3、2、1、92、9W和96。两个座位均有静息等位基因(C4A~*QO和C4B~*QO)存在。它们相应的基因频率为:C4A~*4,0.014;3,0.633;2,0.192;1,0.011;91,0.003;QO,0.147;C4B~*3,0.006;2,0.127;1,0.751;92,0.041;9W,0.003;96,0.006;QO,0.0660单零C4A(c4A QO)表型个体占总体28.3％,单零C4B(C4B QO)占11.1％;纯合C4A缺乏(C4A QO,QO)和纯合C4B缺乏(C4BQO,QO)分别占0.56％。和1.11％卡方测验表明,C4A和C4B基因频率分别符合Hardy-Weinberg遗传平衡定律。