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The genus Hypericum (Hypericaceae) has attracted scientific interest as its members have yielded many bioactive compounds. In the present study we investigated the content of hypericin, pseudohypericin, hyperforin, adhyperforin, chlorogenic acid, neochlorogenic acid, caffeic acid, 2,4-dihydroxybenzoic acid, 13,II8-biapigenin, hyperoside, isoquercitrin, quercitrin, quercetin, avicularin, rutin, (+)-catechin and (−)-epicatechin in aerial parts of plants from populations of H. androsaemum L. and H. polyphyllum Boiss. & Bal. from Turkey growing at different altitudes. The plant materials were dried and subsequently assayed for chemical content by HPLC. All the tested compounds were detected in both species at varying levels depending upon the altitude the plants were growing, except for hypercins and rutin which did not accumulate in H. androsaemum. It was observed that overall the compounds were more abundant in plants from higher altitudes. The differences in the levels of the compounds could contribute to the ability of the plants to deal with the abiotic stress of lower temperature and higher ultraviolet (UV)-B radiation which would be greater at higher altitudes compared to lower altitudes. 相似文献
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Hypericum perforatum L. (St. John’s wort) and Hypericum sampsonii Hance are medicinal plants used in China in the treatment of viruses and other disorders. In the current study, we investigated
the effects of cytokinins 6-benzylaminopurin (BA), zeatin (ZT) and thidiazuron (TDZ) on plant growth and production of hypericins
(pseudohypericin and hypericin) and hyperforin. Our data suggested that culture of H. perforatum in modified MS (Murashige and Skoog) medium, with a 50% reduction in ammonium nitrate and potassium nitrate, and supplemented
with BA (0.44 μM) and indolebutyric acid (IBA, 0.049 μM), resulted in increased production of hypericins. Similar results
were noted with H. sampsonii with minor changes to the medium (0.46 μM ZT and 0.049 μM IBA). There were approximately 2.95-, 2.62-fold increases in H. perforatum pseudohypericin and hypericin production by TDZ (0.45 μM) induction compared to the controls. No enhancement of hypericins
and hyperforin production was elicited by TDZ in H. sampsonii. The elicitor methyl jasmonate (MJA, 50 μM) and its analog, 2,3-dihydroxypropyl jasmonate (DHPJA, 50 μM), were also used
in H. perforatum and H. sampsonii shoot culture to increase secondary metabolite production, eliciting an increase in the production of hypericins and hyperforin.
While leaf senescence and biomass inhibition were observed in cultures induced by MJA, no such effects were observed with
DHPJA. 相似文献
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Jana Koperdáková Hedviga Komarovská Ján Košuth Annalisa Giovannini Eva Čellárová 《Acta Physiologiae Plantarum》2009,31(2):351-358
Hairy root-regenerated clones of Hypericum perforatum L. grown in vitro similarly to those successfully adapted to ex vitro conditions showed phenotype features typical for plants
transformed with Agrobacterium rhizogenes T-DNA. These included reduced apical dominance, increased branching, dwarfing and reduced fertility. Transgenic clones differed
in ability to develop root system as a necessary condition for transfer to the soil. One of the profiling characters, capability
of hypericin biosynthesis was altered as well. Dark glands as the sites of hypericin accumulation and/or synthesis exhibited
significantly higher densities on both, leaves and petals of transgenic clones comparing to controls. In the genome of transgenic
clones, rolABC genes were detected. Both clones harboured similar copy number of individual rol genes. However, copy numbers descended from rolA to rolC gene in both clones. 相似文献
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Xiao-Na Liu Xiu-Qing Zhang Shi-Xiang Zhang Jun-She Sun 《Plant Cell, Tissue and Organ Culture》2007,91(1):1-7
Four precursors (l-phenylalanine, l-tryptophan, cinnamic acid and emodin) and one signal elicitor (methyl jasmonate, MeJA) were added to liquid cultures of Hypericum perforatum L. to study their effect on production of hyperforin and hypericins (pseudohypericin and hypericin). The addition of l-phenylalanine (75 to 100 mg l−1) enhanced production of hypericins, but hyperforin levels were decreased. Hypericin, pseudohypericin and hyperforin concentrations
were all decreased when l-tryptophan (25 to 100 mg l−1) was added to the medium. However, addition of l-tryptophan (50 mg l−1) with MeJA (100 μM) stimulated hyperforin production significantly (1.81-fold) and resulted in an increased biomass. Cinnamic acid (25, 50 mg l−1) and emodin (1.0 to 10.0 mg l−1) each enhanced hyperforin accumulation in H. perforatum, but did not affect accumulation of hypericins. 相似文献
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