全文获取类型
收费全文 | 1006篇 |
免费 | 46篇 |
国内免费 | 28篇 |
出版年
2024年 | 6篇 |
2023年 | 15篇 |
2022年 | 18篇 |
2021年 | 27篇 |
2020年 | 30篇 |
2019年 | 35篇 |
2018年 | 28篇 |
2017年 | 27篇 |
2016年 | 21篇 |
2015年 | 29篇 |
2014年 | 61篇 |
2013年 | 64篇 |
2012年 | 45篇 |
2011年 | 38篇 |
2010年 | 34篇 |
2009年 | 57篇 |
2008年 | 60篇 |
2007年 | 47篇 |
2006年 | 40篇 |
2005年 | 42篇 |
2004年 | 43篇 |
2003年 | 28篇 |
2002年 | 19篇 |
2001年 | 11篇 |
2000年 | 13篇 |
1999年 | 11篇 |
1998年 | 12篇 |
1997年 | 18篇 |
1996年 | 14篇 |
1995年 | 9篇 |
1994年 | 12篇 |
1993年 | 12篇 |
1992年 | 9篇 |
1991年 | 13篇 |
1990年 | 10篇 |
1989年 | 8篇 |
1988年 | 13篇 |
1987年 | 6篇 |
1986年 | 8篇 |
1985年 | 16篇 |
1984年 | 14篇 |
1983年 | 4篇 |
1982年 | 6篇 |
1981年 | 7篇 |
1980年 | 6篇 |
1979年 | 4篇 |
1978年 | 7篇 |
1977年 | 4篇 |
1976年 | 4篇 |
1973年 | 6篇 |
排序方式: 共有1080条查询结果,搜索用时 15 毫秒
1.
Dr. M. Nilsson R. Ekholm G. Fayet S. Maroux L. E. Ericson 《Cell and tissue research》1987,250(2):295-303
Summary The ultrastructural location of aminopeptidase N on the cell surface of isolated porcine thyroid follicle cells was studied with immunocytochemistry using antibodies against intestinal aminopeptidase N and protein A-colloidal gold. Gold particles, indicating immunoreactivity, were selectively attached to the apical cell surface. Occasionally, there was a sparse labelling of the basal cell surface. In follicles kept at 4° C most gold particles at the apical cell surface appeared as clusters, with each gold particle situated at a constant distance of about 20 nm from the membrane surface. The gold particles were concentrated on the membranes of microvilli, in comparison to the smooth (intermicrovillar) portions of the apical plasma membrane. In follicles incubated at 37° C for 5–180 min gold particles were slowly internalized by predominantly smooth-surfaced micropinocytic vesicles and subsequently appeared in colloid droplets and lysosomes. Gold particles were not observed in Golgi cisternae. TSH did not appear to influence the rate of internalization. TSH-induced pseudopods were unlabelled.Our electron-microscopic observations confirm previous immunofluorescence-microscopic evidence that aminopeptidase N is selectively expressed in the apical plasma membrane domain in the thyroid follicle cell. Furthermore, aminopeptidase N appears to be distributed in microdomains within the apical plasma membrane. Earlier indications of molecular differences between the pseudopod membrane and the apical plasma membrane proper are further emphasized.This study was supported by Grant No 12X-537 from the Swedish Medical Research Council 相似文献
2.
Summary The hairs (stereocilia = stereovilli) of sensory cells from the inner ear of vertebrates are interconnected by several types of connectors, whose role is unknown. They appear to stabilize the hair bundle mechanically, and may be directly involved in mechano-electric transduction. Our transmission electron-microscopical investigation of sensory epithelia from two species of fish (Rutilus rutilus, Scardinius erythrophthalmus, both Leuciscidae) has shown that not only the connectors but also the surface charges of the membrane are important factors for determining the shape of the hair bundle and the spatial interrelation of the stereovilli. A reduction of the ionic strength in the medium leads to an increase in distance between the stereovilli. This may be the result of an extension of the spread of the surface potential of the membrane at low ionic strength. The connectors are not broken by the increase in distance between the stereovilli. They are EDTA (ethylene-diamine-tetra-acetic-acid) resistant as are some cell adhesion molecules such as N-CAM (nerve-cell adhesion molecule) and protein A from Dictyostelium discoideum. The connectors do not prevent polycation-induced fusion of adjacent stereovillar membranes. 相似文献
3.
A. Mauger H. Emonard D. J. Hartmann J. M. Foidart P. Sengel 《Development genes and evolution》1987,196(5):295-302
Summary The distribution of various extracellular matrix components was studied in frozen sections of embryonic (14–18 days) and early postnatal (birth and 4 days post parturn) dorsal mouse skin using monospecific antibodies and indirect immunofluorescence. Basement membrane zone components — type IV collagen, laminin and heparan sulphate proteoglycan — were found to be uniformly and unchangingly distributed along the dermal-epidermal junction. In contrast, the distribution of interstitial matrix components — types I and III collagen, and fibronectin — was heterogeneous and varied with the stages of hair development. Collagens became sparse and were eventually completely removed from the prospective dermal papilla and from a one-cell-thick sheath of dermal cells around hair buds. They remained absent from the dermal papilla throughout hair organogenesis. Fibronectin was always present around dermal papilla cells and was particularly abundant along the dermal-epidermal junction of hair rudiments, as well as underneath hair buds. In contrast, in interfollicular skin, collagens accumulated in increasing density, while fibronectin became progressively sparser. It thus appears that interstitial collagens and fibronectin are distributed in a manner which is related to hair morphogenesis. In morphogenetically active regions, collagen density is low, while that of fibronectin is high. Conversely, in histologically stabilized zones, collagen is abundant and fibronectin is sparse. This microheterogeneous distribution of interstitial collagens and of fibronectin might thus constitute part of the morphogenetic message that the dermis is known to transmit to the epidermis during the development of skin and of cutaneous appendages. 相似文献
4.
Dr. P. Gil-Loyzaga R. Pujol R. Mollicone A.-M. Dalix R. Oriol 《Cell and tissue research》1989,257(1):17-21
Summary The presence of human blood-group antigens was analyzed in the rat cochlea during its postnatal development, using anti-A, anti-B and anti-H antibodies. At no stage was reactivity with anti-A antibody observed. With the anti-H antibody, a strong reactivity was observed from 1 to 9 days after birth within hair cells and some other surface epithelial cells of the cochlear duct. After postnatal day 9, only a faint reactivity persisted in a few non-sensory cells. With the anti-B antibody, only hair cells were selectively labeled. At early stages (postnatal day 1 and 3), the reactivity was intense and observed both around the cell surface and within the supranuclear region of cytoplasm. Later on, the reactivity decreased; it was limited at postnatal day 9 to a reactive spot below the cuticular plate. Results are compared with a preliminary finding describing the first appearance of B and H antigens in the organ of Corti at a prenatal stage, and with data concerning other sensory and neural structures. The appearance and progressive disappearance of B and H antigens on sensory and non-sensory cells can be correlated with significant events in the development of the cochlea. The transient expression of B and H antigens in cochlear sensory cells may correspond to developmental changes in their surface glycoconjugates. 相似文献
5.
Günter Kämper Hans-Ulrich Kleindienst 《Journal of comparative physiology. A, Neuroethology, sensory, neural, and behavioral physiology》1990,167(2):193-200
1. | Filiform hairs of various lengths on the cerci of adult crickets vibrate in a sound field. These movements were measured with a photodetector for sound frequencies from 10 Hz to 200 Hz in the species Acheta domestica, Gryllus bimaculatus and Phaeophilacris spectrum. |
2. | With low air-particle velocities, the hair shafts were deflected sinusoidally from their resting position, without bending or secondary oscillations (Figs. 2 A, 3 A). At higher velocities (from ca. 80 mm/s peak velocity, depending on the properties of the individual hairs), the shaft struck the cuticular rim of the socket in which the base of the hair is seated (Fig. 2B). This contact was made at an average angular displacement from the resting position of 5.16°±1.0°. |
3. | The best frequencies of the hairs were found to be between 40 Hz and 100 Hz (Fig. 5A). The slope of the amplitude curve for constant peak air-particle velocity at frequencies below the best frequencies was between 0 and 6 dB/octave. Long hairs had smaller slope values than short hairs (Fig. 5C). |
4. | At its best frequency the ratio of maximal tip displacement of a hair to the displacement of the air particles in the sound field was between 0.2 and 2. Only a small number of hairs (2 out of 36) showed tip displacements exceeding twice the air-particle displacement. The values of maximal angular displacement were not correlated to hair length (Fig. 5 B). |
5. | The angular displacement of the hairs was phase shifted with respect to the air-particle velocity by 0° to +45° (phase lead) at sound frequencies around 10 Hz and by -45° to -120° (phase lag) at 200 Hz (Figs. 3C, 4B). At a particular frequency long hairs tended to have larger phase lags than shorter hairs (Fig. 5D). |
6.
Hair morphology was exammed on developing seedlings and mature plants of ten species from five of seven series inPhysalis. The taxonomic importance of hair development and the distribution of hair types then was evaluated with respect to other comparative data. Two classes of hair types were observed, as found in earlier investigations ofSolanum andRhododendron. The first hair class comprises a sequence from unbranched hairs to dendroid-stelliform hairs. Hairs of this class show considerable variability among taxa in size, presence or absence of glandular tips, and presence and degree of branching, and so were useful in making taxonomic comparisons. The second hair class consists of short hairs, each tipped with a multicellular gland. This second hair class was not useful taxonomically because of its lack of variability in morphology and its often rare occurrence on a plant. The taxa studied exhibit distinctive trichome features which serve to distinguish series and, in some cases together with other data, provided new insight into previously enigmatic species relationships. 相似文献
7.
8.
Culture and characterization of dental follicle cells from rat molars 总被引:12,自引:0,他引:12
Summary Because the dental follicle is necessary for the eruption of teeth of limited eruption, it was the objective of this study to determine if the cells of the follicle could be cultured in vitro. To achieve this, dental follicles and associated enamel organs were dissected from the first and second mandibular molars of 6–7-day-old rats (secretory stage of amelogenesis), and then cultured in a medium that promotes fibroblast growth — the predominant cell type of the dental follicle. The cultured cells grew to confluency and were kept through 3 passages before experimentation. The cultured cells were fibroblastic in shape, elongate with processes, and transmission electron microscopy revealed that they contained an abundant rough endoplasmic reticulum, but did not form desmosomes. Immunofluorescent staining for anti-vimentin showed that all the cells stained and electron-microscopic immunogold labeling indicated that the antibody was associated with intermediate filaments. As revealed by SDS-polyacrylamide gel electrophoresis and Western blotting, the cultured cells synthesized and secreted the extracellular matrix molecules fibronectin and procollagens. Subsequent immunofluorescence staining of permeabilized and non-permeabilized cells confirmed the presence of fibronectin and type I collagen both intra- and extracellularly. Thus, based on all the above characteristics, the cultured cells appeared to be fibroblasts derived from the dental follicle, although a few of the fibroblasts may be derived from undifferentiated mesenchymal cells interposed between the alveolar bone and follicle. Experiments now can be conducted to determine how these cultured cells respond directly to growth factors that alter the rates of tooth eruption. 相似文献
9.
The hair density of free-ranging Japanese monkeys (Macaca fuscata fuscata) living in three different areas was investigated. The Japanese monkeys had thicker hair than other macaques. The hair density
in the Japanese monkeys varied with locality: the northern monkeys had thicker hair than the southern ones. The density did
not vary markedly with age up to 3 years of age, but then decreased gradually up to adult age (≧7 years old). The remarkable
growth of the trunk suggested that the total number of hairs increased with age, especially during the period as a juvenile. 相似文献
10.
Summary The rabbit Graafian follicles are encircled by a capillary network between the theca interna and the avascular membrana granulosa. After injection of an ovulatory dose of human chorionic gonadotrophin (HCG) the theca interna cells showed an increase in the amount of smooth endoplasmic reticulum, lipid droplets and mitochondria with tubular cristae. In addition, considerably more junctions, similar to the abutment nexuses of granulosa cells were found; annular nexuses also appeared. At 4 hours after injection of HCG a prominent oedema was evident in the theca interna layer, particularly in the apical region.Small fenestrations in the endothelium of the blood capillaries increased in amount after HCG injection, and close to the time of ovulation, large gaps or perforations, 1–3 in diameter, were found in the thin, distended part of the endothelial cells. The surrounding basement membrane became fragmented and partly lost, so that a seemingly free passage from the capillary lumen to the interstitium was eventually established. Leakage of fluid, causing interstitial oedema, presumably proceeds until the pressure in the pericapillary interstitium has risen to the pressure in the capillaries. Some hours before and up to ovulation the pericapillary interstitium has also broad communications with the cavity of the follicles. Therefore, both pressure and fluid can be passed from the capillaries-via the interstitium-to the follicle antrum. However, influx of fluid with subsequent follicle expansion and ovulation-at constant pressure-does not occur until the tensile strength of the follicle wall has decreased.This investigation was supported by grants from the Swedish Medical Besearch Council (Projects No. B72-12X-78-07A, B73-12X-78-08B and B74-12X-78-09C). The technical assistance of Miss Ingalis Fransson, Miss Kerstin Nilsson, and Mrs. Ulla-Britt Westman is greatly appreciated. 相似文献