大腹园蛛丝腺的氨基酸组成分析

通过解剖大腹园蛛,获得５种丝腺体,并对各丝腺蛋白进行了氨基酸组成分析和蛋白质相对分子质量测定,比较了不同丝腺体蛋白质组成的差异,发现不同丝腺体蛋白质氨基酸组成中Ｇｌｙ和Ｇｌｕ均有较高含量,而Ｓｅｒ的含量并不高,壶状腺中含有较高的Ｐｒｏ,而管状腺中含有较高的Ａｒｇ．同时发现不同丝腺体蛋白质的相对分子质量存在较明显差异,例如集合腺中不同相对分子质量的蛋白质分布较均匀,而在葡萄状腺中大分子质量的蛋白质明显高于小分子质量蛋白质．     

HPCL2基因表达的选择性剪切分析

2-羟基植烷酸辅酶A裂解酶（2-hydroxypanthyl-CoA lyase,HPCL2）是3-甲基脂肪酸α-氧化途径中的关键酶.采用鸟枪法测序技术,得到了HPCL2基因的基因组序列.结果显示,HPCL2基因组大小为40 829 bp,共有17个外显子,16个内含子.外显子平均大小为116 bp,内含子平均大小为2 429 bp,属于结构紧凑基因.从dbEST数据库中筛选与HPCL2基因的mRNA (GenBank Acc.:AJ131753) 相互重叠表达序列标签(EST)共有213个,分别来自29个不同的组织.将EST与基因组序列进行Blast分析,共检测17个EST具有选择性剪切,其中14例属外显子遗漏（exon skipping）,2例外显子增加 (exon inclusion)和1例剪切位点改变(splicing site shift).外显子遗漏主要发生在5′端的第3到第8个外显子.结果表明,外显子遗漏可能是HPCL2基因选择性剪切的主要形式.     

Determination of natural moisturizing factors in the skin: Raman microspectroscopy versus HPLC

Background: Natural moisturizing factor (NMF) is used as genotypic and phenotypic biomarker in diagnostics. This study is a side-to-side comparison of two different methods to determine NMF in atopic dermatitis patients: Raman microspectroscopy and stratum corneum tape stripping followed by HPLC.

Results: Measured NMF values were significantly correlated (R²?=?.61; p?<?.0001), both methods demonstrated a concentration-depth dependence of NMF and reduced NMF levels in the carriers of filaggrin null mutations. Good agreement between measurements of left and right arms indicated robustness and good reproducibility of both methods.

Conclusions: Both methods showed comparable performance, choice of method will rather be influenced by practical consideration.