Tomato protoplast DNA transformation: physical linkage and recombination of exogenous DNA sequences

Tomato protoplasts have been transformed with plasmid DNA's, containing a chimeric kanamycin resistance gene and putative tomato origins of replication. A calcium phosphate-DNA mediated transformation procedure was employed in combination with either polyethylene glycol or polyvinyl alcohol. There were no indications that the tomato DNA inserts conferred autonomous replication on the plasmids. Instead, Southern blot hybridization analysis of seven kanamycin resistant calli revealed the presence of at least one kanamycin resistance locus per transformant integrated in the tomato nuclear DNA. Generally one to three truncated plasmid copies were found integrated into the tomato nuclear DNA, often physically linked to each other. For one transformant we have been able to use the bacterial ampicillin resistance marker of the vector plasmid pUC9 to rescue a recombinant plasmid from the tomato genome. Analysis of the foreign sequences included in the rescued plasmid showed that integration had occurred in a non-repetitive DNA region. Calf-thymus DNA, used as a carrier in transformation procedure, was found to be covalently linked to plasmid DNA sequences in the genomic DNA of one transformant. A model is presented describing the fate of exogenously added DNA during the transformation of a plant cell. The results are discussed in reference to the possibility of isolating DNA sequences responsible for autonomous replication in tomato.     

藏马鸡(Crossoptilon crossoptilon)的繁殖生态研究

藏马鸡是典型高山地牺雉类,见于海拔3,500—3,900米的针叶林带。自4月中至7月底为繁殖期。一“夫”配一“妻”。营巢于海拔3,544—3,790米的岩洞、灌丛中或倒木下。共发现4巢,3巢已被破坏,成功率25％。近年来,由于过渡砍伐森林,天敌的伤害,自身弱点(繁殖率低,成熟期长(3年左右)以及其他因素,藏马鸡的数量日趋减少,正面临绝种的厄运。必须及时地采取有效保护措施。     

青海藏族青少年骨龄与生长发育关系研究

本文报告了青海省境内,世居在海拔3000-4000米地区的728名7-18岁健康藏族青少年学生的手、腕部骨骼发育情况,对骨化中心出现和骨骺愈合求出了50%出现年龄,并对骨龄与青春期身高突增的关系及与月经初衬潮的关系进行了分析。     

应用PCR技术检测细小病毒H－1DNA在人肝癌与裸鼠正常组织中复制的差异

应用ＰＣＲ技术检测细小病毒Ｈ－１ＤＮＡ在人肝癌与裸鼠正常组织中复制的差异黄青山,马承武,郭兰萍,陈献华,罗祖玉（上海复旦大学生理与生物物理学系,上海２００４３３）关键词：自主性细小病毒Ｈ－１及ＭＶＭ,聚合酶链式反应（ＰＣＲ）,人肝癌模型,抑瘤作用,肿...     

糜子黄、黑种皮遗传及转录组学分析

同源异型域-亮氨酸拉链(homedomain-leucine zipper,HD-Zip)转录因子广泛参与植物的生长发育和抗胁迫过程。该研究通过生物信息学方法对青稞HD-Zip基因家族进行全基因组分析鉴定,并采用qRT-PCR技术分析非生物胁迫下该基因的表达特性,为深入探讨青稞HD-Zip转录因子的生物学功能及其在高原作物抗逆育种中的应用奠定基础。结果表明：(1)成功从青稞基因组中共鉴定出41个HD-Zip基因家族成员,依次命名为i>HvvHD-ZipⅠ-1~Ⅳ-13,且这些基因在7条染色体上呈不均匀分布。(2)理化性质分析发现,HvvHD-Zip蛋白包含197～885个不等的氨基酸残基;分子量范围在19 914.36～94 014.87 Da;亚细胞定位表明HvvHD-Zip蛋白都位于细胞核。(3)根据多序列比对、系统进化、基因结构和保守基序差异将其聚为4个亚家族,各亚家族分类特征与系统聚类结果一致。(4)顺式作用元件预测分析发现,i>HvvHD-Zip基因启动子中含有11种植物激素和胁迫响应元件。(5)qRT-PCR结果显示,HvvHD-Zip Ⅰ、Ⅱ、Ⅳ亚家族基因对各胁迫响应明显;与根组织相比,多数i>HvvHD-Zip基因在叶组织中响应明显(上调或下调);与冷和盐胁迫相比,i>HvvHD-Zip各基因对旱胁迫响应较强。     

黄山雄性短尾猴换群行为的研究

１９８６至１９９２年对安徽黄山一群短尾猴连续进行７年的换群行为研究表明：雄性的换群行为发生在临近交配期开始前后,大多数为成年个体;这些个体与换群前的顺位无关,且未受到迁入新群的其它个体更多的攻击,迁入雄体还倍受雌体青睐。据此推测,外群雌性的性吸引是雄性短尾猴换群的直接原因。雄性短尾猴换群的结果,降低了种群的近亲繁殖率,增加了不同种群间的基因交流,对种群遗传多样性的保存和发展具有重要意义．因此,进化选择压力有利于换群行为的发展。     

短尾猴种群生态学研究：Ⅲ.年龄结构和生命表

本文研究了黄山短猴（Ｍａｃａｃａ ｔｈｉｂｅｔａｎａ）种群年龄结构和雌性生命表。根据毛色、个体大小和行为等指标,可将其种群划分为６个年龄组,即婴猴组、少年组、青年组、年轻成年组、中年组和老年组。雌性短尾猴的静态生命表可较真实地反映了它在自然保护下的自下而上状况,其瞬时增长率（γ）为正值,表明只要加强对短尾猴现有栖息环境的保护,种群才会得到发展。     

拉萨郊区藏族的指纹研究

本文报道了拉萨郊区５１７例（男２２６人,女２９１人）藏族健康人的指纹参数正常值、调查分析了指纹类型、指纹组合、指纹指数和指嵴纹计数。比较了藏族不同居群、不同民族和人种间的差异。结果表明,藏族有自己的指纹特点,又显著蒙古人种的一般特征。     

Alpine grassland plants grow earlier and faster but biomass remains unchanged over 35 years of climate change

Satellite data indicate significant advancement in alpine spring phenology over decades of climate warming, but corresponding field evidence is scarce. It is also unknown whether this advancement results from an earlier shift of phenological events, or enhancement of plant growth under unchanged phenological pattern. By analyzing a 35‐year dataset of seasonal biomass dynamics of a Tibetan alpine grassland, we show that climate change promoted both earlier phenology and faster growth, without changing annual biomass production. Biomass production increased in spring due to a warming‐induced earlier onset of plant growth, but decreased in autumn due mainly to increased water stress. Plants grew faster but the fast‐growing period shortened during the mid‐growing season. These findings provide the first in situ evidence of long‐term changes in growth patterns in alpine grassland plant communities, and suggest that earlier phenology and faster growth will jointly contribute to plant growth in a warming climate.