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Isabelle Bourguin Thierry Chardès Daniel Bout 《FEMS immunology and medical microbiology》1995,12(2):121-126
Abstract Gamma-interferon (IFN-γ) has been reported to be a major mediator of resistance to toxoplasma infection, mainly through macrophage activation. Cholera toxin used as oral adjuvant induces enhanced protection. Following oral immunization of C57BL/6 mice with a Toxoplasma gondii sonicate (TSo), in association with either cholera toxin (CT) or its B subunit (CTB), the ability of primed sensitized peritoneal macrophages (PMgF) to prevent T. gondii intracellular proliferation in vitro was examined both with and without rIFN-γ activation. Under these conditions, the inhibition of T. gondii multiplication was greatly enhanced in PMgF from mice immunized with a TSo and CT as an oral adjuvant. In contrast, PMgF from mice immunized with a TSo in association with CTB showed a decrease in their microbiostatic activity towards T. gondii . This negative effect on IFN-γ-treated PMgF was cancelled out by the addition of a small amount of CT in association with TSo and CTB in the immunization regimen. These data suggest that CT could act as an oral adjuvant in vaccination against toxoplasmosis by increasing the microbiostatic activity of MgF activated with IFN-γ. Further studies, using intestinal effector cells such as enterocytes, are needed to confirm the value of CT for enhancing this major mechanism of protection against T. gondii infection. 相似文献
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