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Species identification and comparative population genetics of four coastal houndsharks based on novel NGS‐mined microsatellites
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Simo N. Maduna Charné Rossouw Charlene da Silva Michelle Soekoe Aletta E. Bester‐van der Merwe 《Ecology and evolution》2017,7(5):1462-1486
The common smooth‐hound (Mustelus mustelus ) is the topmost bio‐economically and recreationally important shark species in southern Africa, western Africa, and Mediterranean Sea. Here, we used the Illumina HiSeq? 2000 next‐generation sequencing (NGS ) technology to develop novel microsatellite markers for Mustelus mustelus . Two microsatellite multiplex panels were constructed from 11 polymorphic loci and characterized in two populations of Mustelus mustelus representative of its South African distribution. The markers were then tested for cross‐species utility in Galeorhinus galeus , Mustelus palumbes , and Triakis megalopterus , three other demersal coastal sharks also subjected to recreational and/or commercial fishery pressures in South Africa. We assessed genetic diversity (N A, A R, H O, H E, and PIC) and differentiation (F ST and D est) for each species and also examined the potential use of these markers in species assignment. In each of the four species, all 11 microsatellites were variable with up to a mean N A of 8, A R up to 7.5, H E and PIC as high as 0.842. We were able to reject genetic homogeneity for all species investigated here except for T . megalopterus . We found that the panel of the microsatellite markers developed in this study could discriminate between the study species, particularly for those that are morphologically very similar. Our study provides molecular tools to address ecological and evolutionary questions vital to the conservation and management of these locally and globally exploited shark species. 相似文献
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The appearance of deformed vertebrae from a single mature female school shark, Galeorhinus galeus , are described. Two unusual, pronounced bumps were noticed in the caudal region of this shark. There were no scars in the skin over these protrusions, suggesting that the lesions had arisen internally. Radiographie and histologie investigation of these lesions showed that mineralized tissue had probably been lost following an injury to the tail. Histological observations provided circumstantial evidence that mineralized tissue had been removed by internal processes, but did not reveal the agency by which skeletal tissue had been resorbed. Since the capacity to resorb mineralized tissue is characteristic of animals possessing bone, the apparent loss of mineralized tissue seen in this shark provides circumstantial evidence for the existence of bone cell lineages in school sharks. This evidence is discussed in relation to the possible implications for evolutionary and fisheries biology. 相似文献
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