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Donald J. Nelson Stephen W. Lafon Thomas E. Jones Thomas Spector Randolph L. Berens J.Joseph Marr 《Biochemical and biophysical research communications》1982,108(1):349-354
Formycin B, a pyrazolo(4,3-d)pyrimidine C-nucleoside, inhibited the growth of promastigotes in culture with an ED90 of 0.2 μg/ml. Promastigotes incubated for 24 hrs with Formycin B at 10 μg/ml were found to convert it to the ribonucleotide, formycin B 5′-monophosphate. The parasites were also capable of aminating formycin B 5′-monophosphate as evidenced by the appearance of formycin A di- and triphosphate. The RNA contained the formycin A moiety in 3′,5′-polynucleotide linkage. Succino-AMP synthetase from these parasites was able to use formycin B 5′-monophosphate as an alternate-substrate with a K'm of 26 μM and a V'm of about 1% the V'm IMP. Formycin B 5′-monophosphate was also a substrate for mammalian succino-AMP synthetase with a Vm' of 40% the Vm' of IMP. 相似文献
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