Haploid plant regeneration from unpollinated ovule cultures of niger (<Emphasis Type="Italic">Guizotia abyssinica</Emphasis> (L. f.) Cass.)

Haploid plants were regenerated in vitro from unpollinated ovules of niger (Guizotia abyssinica (L. f.) (Cass.) on Murashige and Skoog nutrient medium (MS) supplemented with 10 μM naphthaleneacetic acid or 10 μM NAA + 1.5 μM kinetin and 30 g/l sucrose. Gamborg (B5) medium was the best for plant regeneration (in comparison with MS, Nitsch and Nitsch (NN), and Chu (N6) media) from cultured ovules, and 6.66 and 7.33 ovules of JNC-6 and Ootacamund cultivars were involved in direct plant regeneration on this medium. Matured ovules (ovules collected one day before anthesis or on the day of anthesis) only responded to cultural regimes and involved in direct plantlet development. Cytological preparation of root tips and chloroplast counts in the guard cells of leaf stomata of regenerated plants confirmed their haploid nature. This text was submitted by the authors in English.     

Stomatal closure by ultraviolet radiation

The effect of ultraviolet radiation (UV) (255–325 nm) on stomatal closure was investigated on tef [ Eragrostis tef (Zucc) Trotter] in the presence of white light (ca 50 ·mol m⁻² s⁻¹). The action spectrum showed that UV (ca 2 ·mol m⁻² s⁻¹, half band width about 10 nm) of 285 nm or shorter wavelengths was very efficient in causing stomatal closure. The effectiveness decreased sharply towards longer wavelengths. Radiation of 313 nm or longer wavelengths was practically without effect. Increasing UV intensity increased stomatal resistance. When stronger white light (5 to 9 times stronger than the one used during irradiation) was administered, stomates re-opened rapidly irrespective of whether the UV was on or off, although a subsequent gradual closing tendency was observed when the UV was on.     

海甘蓝种子成熟过程中脂肪酸含量以及脱落酸和山梨醇的效应

海甘蓝种子在成熟过程中,棕榈酸、硬脂酸和亚麻酸的含量不断下降,而二十碳烯酸和芥酸的含量呈上升趋势。选用开花后２５～２７ｄ的海甘蓝幼胚分别在含不同浓度的ＡＢＡ或高渗透剂的培养基中培养１～３ｄ,发现其各种脂肪酸的变化趋势和种子自然成熟过程中脂肪酸的变化相似,说明ＡＢＡ或高渗透剂可能是种子成熟过程中各种脂肪酸合成和相互转化所需的条件。     

Effect of heat treatment on the antimicrobial properties of tef dough, injera, kocho and aradisame and the fate of selected pathogens

A known population from each of a 24h culture of Bacillus cereus, Pseudomonas aeruginosa, Salmonella spp., Shigella spp., Klebsiella spp. and Staphylococcus aureus was inoculated into tef flour–water/kocho–water mixtures in screw-capped flasks and allowed to ferment for 30h at room temperature (18–21°C). The flasks were then heat-treated. Cultures of the test bacteria were inoculated into tubes containing graded volumes of 30-h-fermented tef dough/kocho extracts which had been heat-treated at 45, 61 and 80°C in assay broth containing aqueous extracts from injera and aradisame. They were incubated for 24h at 32°C and optical densities determined. Populations of the major indigenous bacteria, yeasts and moulds in fermented tef dough (30h), kocho samples, injera and aradisame were determined from other control portions of the same samples. Higher temperature (80°C) heat-treatment promoted the inhibitory potential of extracts from doughs of both foods as compared with lower temperature heat-treatments (45 and 61°C). Asporogenous test bacteria were affected more than the spore-formers. Better efficacy of extracts from injera and aradisame suggested improved antimicrobial properties of the baked products than in doughs. Heat of baking inactivated all vegetative cells although spores of B.cereus, the yeasts and moulds survived the heat (100°C) applied for 5min. The c.f.u./g of food for B. cereus was below the disease-causing level (0.5×101 and 1.5×103, in injera and aradisame, respectively). Actual baking temperatures in homes are higher than the ones used here; if post-baking contamination is minimized or prevented, the products would be microbiologically safe with respect to the asporogenous pathogens when served fresh. Further studies on aflatoxins and improved storage conditions for kocho are recommended.     

高芥酸油料植物海甘蓝的引种和利用

本文对高芥酸油料植物海甘蓝的栽培特性、农业生物学特性、种子成分以及栽培措施进行综合报道,并对其产物的利用和芥酸的工业用途进行了讨论。     

Establishing genomic tools and resources for Guizotia abyssinica (L.f.) Cass.-the development of a library of expressed sequence tags, microsatellite loci, and the sequencing of its chloroplast genome

We present an EST library, chloroplast genome sequence, and nuclear microsatellite markers that were developed for the semi-domesticated oilseed crop noug (Guizotia abyssinica) from Ethiopia. The EST library consists of 25 711 Sanger reads, assembled into 17 538 contigs and singletons, of which 4781 were functionally annotated using the Arabidopsis Information Resource (TAIR). The age distribution of duplicated genes in the EST library shows evidence of two paleopolyploidizations—a pattern that noug shares with several other species in the Heliantheae tribe (Compositae family). From the EST library, we selected 43 microsatellites and then designed and tested primers for their amplification. The number of microsatellite alleles varied between 2 and 10 (average 4.67), and the average observed and expected heterozygosities were 0.49 and 0.54, respectively. The chloroplast genome was sequenced de novo using Illumina’s sequencing technology and completed with traditional Sanger sequencing. No large re-arrangements were found between the noug and sunflower chloroplast genomes, but 1.4% of sites have indels and 1.8% show sequence divergence between the two species. We identified 34 tRNAs, 4 rRNA sequences, and 80 coding sequences, including one region (trnH-psbA) with 15% sequence divergence between noug and sunflower that may be particularly useful for phylogeographic studies in noug and its wild relatives.     

High-Throughput Discovery of Mutations in Tef Semi-Dwarfing Genes by Next-Generation Sequencing Analysis

Tef (Eragrostis tef) is a major cereal crop in Ethiopia. Lodging is the primary constraint to increasing productivity in this allotetraploid species, accounting for losses of ∼15–45% in yield each year. As a first step toward identifying semi-dwarf varieties that might have improved lodging resistance, an ∼6× fosmid library was constructed and used to identify both homeologues of the dw3 semi-dwarfing gene of Sorghum bicolor. An EMS mutagenized population, consisting of ∼21,210 tef plants, was planted and leaf materials were collected into 23 superpools. Two dwarfing candidate genes, homeologues of dw3 of sorghum and rht1 of wheat, were sequenced directly from each superpool with 454 technology, and 120 candidate mutations were identified. Out of 10 candidates tested, six independent mutations were validated by Sanger sequencing, including two predicted detrimental mutations in both dw3 homeologues with a potential to improve lodging resistance in tef through further breeding. This study demonstrates that high-throughput sequencing can identify potentially valuable mutations in under-studied plant species like tef and has provided mutant lines that can now be combined and tested in breeding programs for improved lodging resistance.     

Native Alternatives for Non-Native Turfgrasses in Central Florida: Germination and Responses to Cultural Treatments

Little information exists about the establishment of native longleaf pine flatwoods species for use in restoration efforts and as buffers around natural areas in the southeastern United States. Composition of groundcover in these systems is dominated by perennial graminoid species. Vegetation in current buffers is generally non‐native turfgrass that can escape into natural areas, often reducing establishment and survival of native species. Where management objectives involve actively restoring native groundcover or reducing the probability of invasion by these non‐native turfgrasses, identification of native species and restoration methods is needed. We investigated seed germination and establishment of four species native to longleaf pine flatwoods in central Florida and one species native to the adjacent wetland communities. Paspalum setaceum, Panicum anceps, Eustachys petraea, and Eragrostis refracta were directly seeded, and P. distichum was planted as sprigs into three former P. notatum pastures. Irrigation, fertilization, weed control, and mowing treatments were assessed in terms of cover development of the sown species. Paspalum distichum developed the highest percent cover—over 80% in wet areas after 1 year. Mowing had mixed impacts depending on the species, and fertilization never significantly increased cover. Directly seeded species developed sparse cover (0–40%), probably as a result of drought conditions. However, E. petraea and E. refracta appeared more promising for use on rights‐of‐way when using high sowing rates. A second experiment conducted on a roadside included these two species and sprigged P. distichum. Both E. petraea and P. distichum developed more than 45% cover on the roadside. Establishment of these natives from seed or sprigs was significantly enhanced when site preparation effectively reduced the seedbank of other species present in the soil.     

How does the C4 grass Eragrostis pilosa respond to elevated carbon dioxide and infection with the parasitic angiosperm Striga hermonthica?

Eragrostis pilosa (Linn.) P Beauv., a C₄ grass native to east Africa, was grown at both ambient (350 μmol mol⁻¹ and elevated (700 μmol mol⁻¹) CO₂ in either the presence or absence of the obligate, root hemi-parasite Striga hermonthica (Del.) Benth. Biomass of infected grasses was only 50% that of uninfected grasses at both CO₂ concentrations, with stems and reproductive tissues of infected plants being most severely affected. By contrast, CO₂ concentration had no effect on growth of E. pilosa , although rates of photosynthesis were enhanced by 30–40% at elevated CO₂. Infection with S. hermonthica did not affect either rates of photosynthesis or leaf areas of E. pilosa , but did bring about an increase in root:shoot ratio, leaf nitrogen and phosphorus concentration and a decline in leaf starch concentration at both ambient and elevated CO₂. Striga hermonthica had higher rates of photosynthesis and shoot concentrations of soluble sugars at elevated CO₂, but there was no difference in biomass relative to ambient grown plants. Both infection and growth at elevated CO₂ resulted in an increase in the Δ¹³C value of leaf tissue of E. pilosa , with the CO₂ effect being greater. The proportion of host-derived carbon in parasite tissue, as determined from δ¹³C values, was 27% and 39% in ambient and elevated CO₂ grown plants, respectively. In conclusion, infection with S. hermonthica limited growth of E. pilosa , and this limitation was not removed or alleviated by growing the association at elevated CO₂.