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Aixia Li Wenlong Yang Xueyuan Lou Dongcheng Liu Jiazhu Sun Xiaoli Guo Jing Wang Yiwen Li Kehui Zhan Hong-Qing Ling Aimin Zhang 《植物学报(英文版)》2013,55(11):1026-1037
Plant height is an important agronomic trait. Dramatic increase in wheat yield during the "green revolution" is mainly due to the widespread utilization of the Reduced height (Rht)-1gene. We analyzed the natural allelic variations of three homoeologous loci Rht-A1, Rht-B1, and Rht-D1 in Chinese wheat (Triticum aestivum L.) micro-core collections and the Rht-B1/D1 genotypes in over 1,500 bred cultivars and germplasms using a modified EcoTILLING. We identified six new Rht-A1 allelic variations (Rht-Alb-g), eight new Rht-B1 allelic variations (Rht-Blh-o), and six new Rht-D1 allelic variations (Rht-Dle-j). These allelic variations contain single nucleotide polymorphisms (SNPs) or small insertions and deletions in the coding or uncoding regions, involving two frame-shift mutations and 15 missenses. Of which, Rht-Dle and Rht-Dlh resulted in the loss of interactions of GID1-DELLA-GID2, Rht-Blicould increase plant height. We found that the Rht-Blh contains the same SNPs and 197 bp fragment insertion as reported in Rht-Blc. Further detection of Rht-Blh in Tibet wheat germplasms and wheat relatives indicated that Rht-Blc may originate from Rht-Blh. These results suggest rich genetic diversity at the Rht-1 loci and provide new resources for wheat breeding. 相似文献
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EcoTILLING技术是由TILLING技术发展而来的快速发现和确定自然群体中等位基因多态性的方法,是一种全新的反向遗传学研究方法。本文介绍了EcoTILLING技术的特点及其应用现状和前景。 相似文献
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Rapid method for detecting SNPs on agarose gels and its application in candidate gene mapping 总被引:6,自引:0,他引:6
Chitra Raghavan Ma. Elizabeth B. Naredo Hehe Wang Genelou Atienza Bin Liu Fulin Qiu Kenneth L. McNally Hei Leung 《Molecular breeding : new strategies in plant improvement》2007,19(2):87-101
TILLING (Targeting Induced Local Lesions IN Genomes) exploits the fact that CEL I endonuclease cleaves heteroduplexes at positions
of single nucleotide or small indel mismatches. To detect single nucleotide polymorphisms (SNPs) across a population, DNA
pools are created and a target locus under query is PCR-amplified and subjected to heteroduplex formation, followed by CEL
I cleavage. Currently, the common method used to detect cleaved products is by polyacrylamide gel electrophoresis using a
high-throughput genotyping platform. Exact SNPs are then determined by sequencing. We sought to simplify the detection of
CEL I-cleaved products on conventional agarose gels to make the technique more accessible to collaborating partners in developing
countries where access to instrumentation could be limiting. Here, we used a panel of stress-related genes to evaluate SNP
detection across 48 rice genotypes by contrasting them individually against IR64 and Nipponbare. SNP detection calls corresponded
perfectly with those obtained from the Li-Cor genotypers. We were able to detect SNPs in pools of eight DNA templates, suggesting
that the agarose gel system could be used to screen for SNPs with comparable throughput as that of the Li-Cor genotypers and
showed that the throughput can be increased by analyzing larger amplicons (∼3 kb). The agarose method offers a significant
advantage by alleviating the need for labeled primers. We further demonstrated that the agarose method can be effectively
used in gene mapping, an application particularly useful for parental lines with low levels of polymorphism. The lower cost
and simplicity of the technique make it possible for broader applications of SNP-based markers for germplasm characterization
and mapping studies. 相似文献
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M. Laura Ramos James J. Huntley Soheila J. Maleki Peggy Ozias-Akins 《Plant molecular biology》2009,69(3):325-335
Peanut (Arachis hypogaea L.), can elicit type I allergy becoming the most common cause of fatal food-induced anaphylactic reactions. Strict avoidance
is the only effective means of dealing with this allergy. Ara h 2, a peanut seed storage protein, has been identified as the
most potent peanut allergen and is recognized by approximately 90% of peanut hypersensitive individuals in the US. Because
peanut has limited genetic variation, wild relatives are a good source of genetic diversity. After screening 30 Arachis duranensis accessions by EcoTILLing, we characterized five different missense mutations in ara d 2.01. None of these polymorphisms induced major conformational modifications. Nevertheless, a polymorphism in the immunodominant
epitope #7 (S73T) showed a 56–99% reduction in IgE-binding activity and did not affect T cell epitopes, which must be retained
for effective immunotherapy. The identification of natural hypoallergenic isoforms positively contributes to future immunological
and therapeutic studies and peanut cultivar development.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
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Aixia Li Wenlong Yang Xueyuan Lou Dongcheng Liu Jiazhu Sun Xiaoli Guo Jing Wang Yiwen Li Kehui Zhan HongQing Ling Aimin Zhang 《植物学报(英文版)》2013,55(11):1026-1037
Plant height is an important agronomic trait. Dramatic increase in wheat yield during the“green revolution”is mainly due to the widespread utilization of the Reduced height (Rht)-1 gene. We analyzed th... 相似文献
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