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排序方式: 共有131条查询结果,搜索用时 15 毫秒
1.
Hydraulic control of stomatal conductance in Douglas fir [Pseudotsuga menziesii (Mirb.) Franco] and alder [Alnus rubra (Bong)] seedlings 总被引:1,自引:0,他引:1
Experiments were conducted on 1-year-old Douglas fir [Pseudotsuga menziesii (Mirb.) Franco] and 2- to 3-month-old alder [Alnus rubra (Bong)] seedlings growing in drying soils to determine the relative influence of root and leaf water status on stomatal conductance (gc). The water status of shoots was manipulated independently of that of the roots using a pressure chamber that enclosed the root system. Pressurizing the chamber increases the turgor of cells in the shoot but not in the roots. Seedling shoots were enclosed in a whole-plant cuvette and transpiration and net photosynthesis rates measured continuously. In both species, stomatal closure in response to soil drying was progressively reversed with increasing pressurization. Responses occurred within minutes of pressurization and measurements almost immediately returned to pre-pressurization levels when the pressure was released. Even in wet soils there was a significant increase in gc with pressurization. In Douglas fir, the stomatal response to pressurization was the same for seedlings grown in dry soils for up to 120 d as for those subjected to drought stress over 40 to 60 d. The stomatal conductance of both Douglas fir and alder seedlings was less sensitive to root chamber pressure at higher vapour pressure deficits (D), and stomatal closure in response to increasing D from 1.04 to 2.06 kPa was only partially reversed by pressurization. Our results are in contrast to those of other studies on herbaceous species, even though we followed the same experimental approach. They suggest that it is not always appropriate to invoke a ‘feedforward’ model of short-term stomatal response to soil drying, whereby chemical messengers from the roots bring about stomatal closure. 相似文献
2.
Physiological effects of five months exposure to low concentrations of O3 and NH3 on Douglas fir (Pseudotsuga menziesii) 总被引:2,自引:0,他引:2
Three years old seedlings of Douglas fir (Pseudotsuga menziesii) were exposed lo filtered air, O3 (day and night concentrations of 78 and 30 μgm?3: respectively). NH3 (54 μg m?3) and to a mixture of NH3+O3 (day and night concentrations of 49 + 83 and 49 + 44 μg m?3 respectively), for 5 months in fumigation chambers. Both gas exchange and chlorophyll fluorescence were measured on shoots which had sprouted at the beginning of the exposure period. After 4. 8, 10 and 20 weeks of exposure, light response curves of electron transport rate (J) were determined, in which J was deduced from chlorophyll fluorescence. Net CO2 assimiialion was measured at maximum light intensity of 560) μmol m?2 S?1 (Pn.560). After 8 and 10 weeks of exposure also light response curves of CO2 assimilation were assessed. Shoots exposed to O3 showed a reduction in net CO2 assimilation as compared to the control shoots during the entire exposure period. The reduction was related lo a lower chlorophyll content and a lower electron transport rate, whereas no effect on quantum yield efficiency (qy) was observed. In contrast, shoots exposed to NH3 showed a positive effect on photosynthesis. Shoots exposed to NH3. + O3 showed a rapid increase in Pn.560, in the period between 4 and 8 weeks to a level equal of that of the NH3-treatment. After this period a decline in Pn.560 was observed. After 10 weeks of exposure shoots exposed to O3 showed an increased transpiration rate in the dark as compared to the control shoots. In addition, water use efficiency (WUE) declined as a result of an increase in leaf conductance. Both observations indicate that the stomatal apparatus was affected by O3. A high transpiration rate in the dark was also found for shoots esposed to NHX. However, shoots exposed to NH3+ O3 showed neither an effect on WUE, nor an effect on transpiration rate in the dark. The possibility that NH3 delayed the O3 induced effects on photosynthesis and stomatal conductance is discussed. 相似文献
3.
G. Pilate B. Sotta R. Maldiney M. Jacques L. Sossountzov E. Miginiac 《Physiologia plantarum》1989,76(1):100-106
Bud quiescence release, considered as the ultimate dormancy breaking phase, was achieved in Pseudotsuga menziesii (Mirb.) Franco by a 9-week cold (5°C) treatment, under short daylength (9 h) followed by a transfer to mild temperature (22°C) under long daylength (16 h). Indole-3-acetic acid (IAA), abscisic acid (ABA), zeatin-type (Z) and isopentenyladenine-type (iPA) cytokinin (CK) levels were measured by means of an ELISA technique performed on HPLC-fractionated extracts of terminal and axillary buds. During the cold period, all hormones except IP-type CK levels decreased, whereas the opposite observation was made after transfer to mild temperature and long daylength, when buds started to grow. Some other immunoreactive compounds were also detected and quantified. The ABA-glucosyl ester (ABA-GE) level pattern was similar to that of ABA, but no accumulation occurred at mild temperatures. A putative IAA conjugate, more polar than IAA, was also detected. Its level increased transiently like IAA in terminal buds and, to a lesser extent, in axillary buds during the 10th week of the experiment. In terminal buds, isopentenyladenosine ([9R]-iP) was released by alkaline hydrolysis of a polar immunoreactive compound detected with anti-[9R]iP antibodies. This compound accumulated during the cold period and quickly dropped at 22°C. Relationships between environmental conditions and endogenous hormones are discussed. 相似文献
4.
Pieter H. B. De Visser Willem G. Keltjens Günther R. Findenegg 《Trees - Structure and Function》1996,10(5):301-307
In a pot trial growth and transpiration of 3-year-old Douglas-fir seedlings on an acid, sandy soil was examined at a deficient
(30 kg N ha –
1 year –
1) and an excessive level (120 kg N ha –
1 year –
1) of NH4 application. Dissolved ammonium sulphate was applied to the pots weekly for two growing seasons. In half of the pots a complete
set of other nutrients was applied in optimal proportions to the applied nitrogen. Water supply was optimal and transpiration
was recorded. At the end of the second treatment season irrigation was stopped for 2 weeks during dry and sunny weather. Both
high application of NH4 and additional nutrients increased shoot growth and transpiration demand in the first treatment year. The root system was
smaller at higher N level and this reduced water uptake accordingly. In the second year the combination of high NH4
+ and additional nutrients affected root functioning predominantly due to salinity effects and this seriously decreased water
uptake capacity and shoot water potentials, finally resulting in tree death. Without addition of other nutrients the high
NH4
+ application resulted in a high degree of soil acidification, which damaged the roots, that showed a decrease in water uptake
capacity. At the low NH4 supply level soil acidification was lower, and root functioning was not affected, and the trees recovered quickly from the
imposed drought. Higher needle K and P status depressed transpiration rates at the low NH4 application rate.
Received: 9 January 1995 / Accepted: 18 September 1995 相似文献
5.
6.
The endoplasmic reticulum (ER) molecular chaperone, BiP, plays a role in the cotranslational translocation and subsequent
folding and assembly of newly synthesized proteins targeted to the ER and secretory pathway. The sequence encoding a Douglas-fir
(Pseudotsuga menziesii [Mirb] Franco) BiP homologue (PmBiP) was identified by differential screening of a seedling cDNA library. Southern blotting
indicated that PmBiP is most likely present as a single copy. The deduced amino acid sequence of PmBiP contains an HEEL tetrapeptide
sequence which functions to retain PmBiP in the ER and is different from HDEL commonly found in angiosperm plant BiPs. Amino
acid sequence alignment and phylogenetic analysis show that PmBiP is highly similar to other plant BiPs yet forms a distinct
phylogenetic subgroup which is separate from the angiosperm BiPs. Northern and western blotting revealed that PmBiP is subject
to developmental regulation during seed development, germination, and early seedling growth and is seasonally regulated in
needles of young seedlings.
Received: 21 February 2000 / Accepted: 13 April 2000 相似文献
7.
William L. Baker Thomas T. Veblen Rosemary L. Sherriff † 《Journal of Biogeography》2007,34(2):251-269
Aim Forest restoration in ponderosa pine and mixed ponderosa pine–Douglas fir forests in the US Rocky Mountains has been highly influenced by a historical model of frequent, low‐severity surface fires developed for the ponderosa pine forests of the Southwestern USA. A restoration model, based on this low‐severity fire model, focuses on thinning and prescribed burning to restore historical forest structure. However, in the US Rocky Mountains, research on fire history and forest structure, and early historical reports, suggest the low‐severity model may only apply in limited geographical areas. The aim of this article is to elaborate a new variable‐severity fire model and evaluate the applicability of this model, along with the low‐severity model, for the ponderosa pine–Douglas fir forests of the Rocky Mountains. Location Rocky Mountains, USA. Methods The geographical applicability of the two fire models is evaluated using historical records, fire histories and forest age‐structure analyses. Results Historical sources and tree‐ring reconstructions document that, near or before ad 1900, the low‐severity model may apply in dry, low‐elevation settings, but that fires naturally varied in severity in most of these forests. Low‐severity fires were common, but high‐severity fires also burned thousands of hectares. Tree regeneration increased after these high‐severity fires, and often attained densities much greater than those reconstructed for Southwestern ponderosa pine forests. Main conclusions Exclusion of fire has not clearly and uniformly increased fuels or shifted the fire type from low‐ to high‐severity fires. However, logging and livestock grazing have increased tree densities and risk of high‐severity fires in some areas. Restoration is likely to be most effective which seeks to (1) restore variability of fire, (2) reverse changes brought about by livestock grazing and logging, and (3) modify these land uses so that degradation is not repeated. 相似文献
8.
Facilitation of tree establishment by ectomycorrhizal (EM) networks (MNs) may become increasingly important as drought stress increases with climate change in some forested regions of North America. The objective of this study was to determine (1) whether temperature, CO(2) concentration ([CO(2)]), soil moisture, and MNs interact to affect plant establishment success, such that MNs facilitate establishment when plants are the most water stressed, and (2) whether transfer of C and water between plants through MNs plays a role in this. We established interior Douglas-fir (Pseudotsuga menziesiivar.glauca) seedlings in root boxes with and without the potential to form MNs with nearby conspecific seedlings that had consistent access to water via their taproots. We varied temperature, [CO(2)], and soil moisture in growth chambers. Douglas-fir seedling survival increased when the potential existed to form an MN. Growth increased with MN potential under the driest soil conditions, but decreased with temperature at 800 ppm [CO(2)]. Transfer of (13)C to receiver seedlings was unaffected by potential to form an MN with donor seedlings, but deuterated water (D(2)O) transfer increased with MN potential under ambient [CO(2)]. Chlorophyll fluorescence was reduced when seedlings had the potential to form an MN under high [CO(2)] and cool temperatures. We conclude that Douglas-fir seedling establishment in laboratory conditions is facilitated by MN potential where Douglas-fir seedlings have consistent access to water. Moreover, this facilitation appears to increase as water stress potential increases and water transfer via networks may play a role in this. These results suggest that conservation of MN potential may be important to forest regeneration where drought stress increases with climate change. 相似文献
9.
D. J. Lodge 《The New phytologist》2000,146(3):353-354
Ecological theories concerning the relationship of suites of traits with resource supply and plant potential growth rate have concentrated primarily on leaf structure and physiology – much less is known about interspecific patterns of variation in root structure and physiology. In this issue, Wahl & Ryser reduce this knowledge gap by demonstrating important correlations of root structure with potential growth rate and height among a range of perennial temperate grass species adapted to sites of a wide range of fertility (see pp. 459–471). 相似文献
10.
A method for direct estimation of percentage apoplastic water volume (% APO) in conifer needles is described. The method presented here, and designated the pressure-needle (P-N) method, measures the relative water content of the needles to develop a curve similar to the pressure-volume (P-V) curve. P-V and P-N curves were developed for Picea pungens Engelm. cv. Hoopsi, Pinus sylvestris L., Abies gradis (Dougl.) L., and Pseudotsuga menziesii (Mirb) Franco. The % APO estimated by the two procedures varied as much as 2-fold, while other parameters were similar. The P-V method generated consistently higher and more variable % APO than the P-N method, due to the inclusion of the apoplastic water of the stem in the P-V method. For conifers, the P-N method offers a more accurate and precise method for determining % APO. 相似文献