排序方式: 共有14条查询结果,搜索用时 15 毫秒
1.
JuKyung Lee SiHyeong Cho JungHwan Lee HeonYul Ryu JinGoo Park SunHee Lim ByungDo Oh ChangWoo Lee Wilber Huang Ahmed Busnaina HeaYeon Lee 《Journal of biotechnology》2013
We have reported that nanowell array (NWA) can enhance electrochemical detection of molecular binding events by controlling the binding sites of the captured molecules. Using NWA biosensor based amperometric analysis, we have detected biological macromolecules such as DNA, protein or aptamers at low concentrations. In this research, we developed an impedimetric immunosensor based on wafer-scale NWA for electrochemical detection of stress-induced-phosphoprotein-1 (STIP-1). In order to develop NWA sensor through the cost-effective combination of high-throughput nanopattern, the NWA electrode was fabricated on Si wafer by krypton-fluoride (KrF) stepper semiconductor process. Finally, 12,500,000 ea nanowell with a 500 nm diameter was fabricated on 4 mm × 2 mm substrate. Next, by using these electrodes, we measured impedance to quantify antigen binding to the immunoaffinity layer. The limit of detection (LOD) of the NWA was improved about 100-fold compared to milli-sized electrodes (4 mm × 2 mm) without an NWA. These results suggest that wafer-scale NWA immunosensor will be useful for biosensing applications because their interface response is appropriate for detecting molecular binding events. 相似文献
2.
Gold nanoparticles were used to enhance the immobilization amount and retain the immunoactivity of recombinant dust mite allergen Der f2 immobilized on a glassy carbon electrode (GCE). The interaction between allergen and antibody was studied by electrochemical impedance spectroscopy (EIS). Self-assembled Au colloid layer (?=16nm) deposited on (3-mercaptopropyl)trimethoxysilane (MPTS)-modified GCE offered a basis to control the immobilization of allergen Der f2. The impedance measurements were based on the charge transfer kinetics of the [Fe(CN)(6)](3-/4-) redox pair, compared with bare GCE, the immobilization of allergen Der f2 and the allergen-antibody interaction that occurred on the electrode surface altered the interfacial electron transfer resistance and thereby slowed down the charge transfer kinetics by reducing the active area of the electrode or by preventing the redox species in electrolyte solution from approaching the electrode. The interactions of allergen with various concentrations of monoclonal antibody were also monitored through the change of impedance response. The results showed that the electron transfer resistance increased with increasing concentrations of monoclonal antibody. 相似文献
3.
Whittaker SB Spence GR Günter Grossmann J Radford SE Moore GR 《Journal of molecular biology》2007,366(3):1001-1015
Previous work shows that the transiently populated, on-pathway intermediate in Im7 folding contains three of the four native alpha-helices docked around a core stabilised by native and non-native interactions. To determine the structure and dynamic properties of this species in more detail, we have used protein engineering to trap the intermediate at equilibrium and analysed the resulting proteins using NMR spectroscopy and small angle X-ray scattering. Four variants were created. In L53AI54A, two hydrophobic residues within helix III are truncated, preventing helix III from docking stably onto the developing hydrophobic core. In two other variants, the six residues encompassing the native helix III were replaced with three (H3G3) or six (H3G6) glycine residues. In the fourth variant, YY, two native tyrosine residues (Tyr55 and Tyr56) were re-introduced into H3G6 to examine their role in determining the properties of the intermediate ensemble. All four variants show variable peak intensities and broad peak widths, consistent with these proteins being conformationally dynamic. Chemical shift analyses demonstrated that L53AI54A and YY contain native-like secondary structure in helices I and IV, while helix II is partly formed and helix III is absent. Lack of NOEs and rapid NH exchange for L53AI54A, combined with detailed analysis of the backbone dynamics, indicated that the hydrophobic core of this variant is not uniquely structured, but fluctuates on the NMR timescale. The results demonstrate that though much of the native-like secondary structure of Im7 is present in the variants, their hydrophobic cores remain relatively fluid. The comparison of H3G3/H3G6 and L53AI54A/YY suggests that Tyr55 and/or Tyr56 interact with the three-helix core, leading other residues in this region of the protein to dock with the core as folding progresses. In this respect, the three-helix bundle acts as a template for formation of helix III and the creation of the native fold. 相似文献
4.
Previous work shows that Im9 folds in a two-state transition while its homologue Im7 folds in a three-state transition via an on-pathway kinetic intermediate state (KIS), with this difference being related to frustration in the structure of Im7. We have used NMR spectroscopy to study conformational dynamics connected to the frustration. A combination of equilibrium peptide N1H/N2H exchange, model-free analyses of backbone NH relaxation data and relaxation dispersion (RD)-NMR shows that the native state of Im7 is in equilibrium with an intermediate state that is lowly populated [equilibrium intermediate state (EIS)]. Comparison of kinetic and thermodynamic parameters describing the EIS native-state equilibrium obtained by RD-NMR with previously reported parameters describing the KIS native-state equilibrium obtained from stopped-flow fluorescence studies of refolding His-tagged Im7 shows that the KIS and the EIS are the same species. 15N chemical shifts of the EIS obtained from the RD-NMR analysis show that residues forming helix III in the native state are unstructured in the EIS while other residues experiencing frustration in the native state are in structured regions of the EIS. We show that binding of Im7 and its L53A/I54A variant (which resembles the EIS as shown in previous work) to the cognate partner for Im7, the DNase domain of colicin E7, causes the dynamic processes associated with the frustration to be dampened. 相似文献
5.
Qidong Li Li Li Peijie Wu Nuo Xu Liang Wang Matthew Li Alvin Dai Khalil Amine Liqiang Mai Jun Lu 《Liver Transplantation》2019,9(43)
Nickel sulfides are regarded as promising anode materials for advanced rechargeable lithium‐ion batteries due to their high theoretical capacity. However, capacity fade arising from significant volume changes during operation greatly limits their practical applications. Herein, confined NiSx@C yolk–shell microboxes are constructed to address volume changes and confine the active material in the internal void space. Having benefited from the yolk–shell structure design, the prepared NiSx@C yolk–shell microboxes display excellent electrochemical performance in lithium‐ion batteries. Particularly, it delivers impressive cycle stability (460 mAh g?1 after 2000 cycles at 1 A g?1) and superior rate performance (225 mAh g?1 at 20 A g?1). Furthermore, the lithium storage mechanism is ascertained with in situ synchrotron high‐energy X‐ray diffractions and in situ electrochemical impedance spectra. This unique confined yolk–shell structure may open up new strategies to create other advanced electrode materials for high performance electrochemical storage systems. 相似文献
6.
Enhancement of hydrogen production in a single chamber microbial electrolysis cell through anode arrangement optimization 总被引:1,自引:0,他引:1
Reducing the inner resistances is crucial for the enhancement of hydrogen generation in microbial electrolysis cells (MECs). This study demonstrates that the optimization of the anode arrangement is an effective strategy to reduce the system resistances. By changing the normal MEC configuration into a stacking mode, namely separately placing the contacted anodes from one side to both sides of cathode in parallel, the solution, biofilm and polarization resistances of MECs were greatly reduced, which was also confirmed with electrochemical impedance spectroscopy analysis. After the anode arrangement optimization, the current and hydrogen production rate (HPR) of MEC could be enhanced by 72% and 118%, reaching 621.3 ± 20.6 A/m3 and 5.56 m3/m3 d respectively, under 0.8 V applied voltage. A maximum current density of 1355 A/m3 with a HPR of 10.88 m3/m3 d can be achieved with 1.5 V applied voltage. 相似文献
7.
以菊花(Chrysanthemum morifolium)‘神马’扦插苗茎段和叶片为材料,测定其高温胁迫(25~50℃)下电阻抗图谱参数的变化,通过膜透性(相对电导率)与电阻抗图谱参数间的相关性,来证明电阻抗图谱法研究菊花耐热性的有效性。结果表明:随着温度升高,茎叶的胞外电阻、胞内电阻、弛豫时间呈现先增加,后急剧下降的趋势,而弛豫时间分布系数变化趋势为不规则下降。茎叶相对电导率是随着温度的升高先增加后降低再急剧增加。经过菊花茎叶电阻抗图谱参数拟合菊花的耐热性与相对电导率表示的耐热性温度极为接近。相关分析表明,高温胁迫下菊花茎叶相对电导率与胞外电阻(茎p<0.01,叶p<0.05)、弛豫时间(p<0.05)之间有显著的相关。由此可见,胞外电阻和弛豫时间是测定菊花耐热性的适合参数。 相似文献
8.
Ultrasensitive DNA sensor based on gold nanoparticles/reduced graphene oxide/glassy carbon electrode
Ali Benvidi Afsaneh Dehghani Firouzabadi Seyed Mohammad Moshtaghiun Mohammad Mazloum-Ardakani Marzieh Dehghan Tezerjani 《Analytical biochemistry》2015
We have designed a simple and novel electrochemical biosensor based on glassy carbon electrode (GCE) for DNA detection. GCE was modified with reduced graphene oxide (RGO) and gold nanoparticles (AuNPs) by the electrochemical method, which is helpful for immobilization of thiolated bioreceptors. The electrode modification processes were characterized by scanning electron microscopy (SEM) and electrochemical methods. Then a single-stranded DNA (ssDNA) probe for BRCA1 5382 insC mutation detection was immobilized on the modified electrode for a specific time. The experimental conditions, such as probe immobilization time and target DNA (complementary DNA) hybridization time and temperature with probe DNA, were optimized using electrochemical methods. The electrochemical response for DNA hybridization and synthesis was measured using electrochemical impedance spectroscopy (EIS) and cyclic voltammetry (CV) methods. The calibration graph contains two linear ranges; the first part is in the range of 3.0 × 10−20 to 1.0 × 10−12 M, and the second segment part is in the range of 1.0 × 10−12 to 1.0 × 10−7 M. The biosensor showed excellent selectivity for the detection of the complementary sequences from noncomplementary sequences, so it can be used for detection of breast cancer. 相似文献
9.
Electrochemically fabricated polypyrrole nanofiber-modified electrode as a new electrochemical DNA biosensor 总被引:4,自引:0,他引:4
A new biosensor employing immobilized DNA on a nano-structured conductive polymer fixed onto a platinum electrode is presented. Upon optimization of synthesis parameters, polypyrrole nanofibers, 30-90 nm in diameter, were synthesized in an aqueous media by the electropolymerization of pyrrole using normal pulse voltammetry (NPV). The nanofiber film was investigated by scanning electron microscopy (SEM), cyclic voltammetry (CV) and electrochemical impedance spectroscopy (EIS). Double-stranded DNA was physisorbed onto the PPy nanofiber films. Various parameters, including the pH and DNA concentration, were optimized. The DNA immobilized on the nanofiber films was characterized using differential pulse voltammetry (DPV) and Fourier-transform infrared (FTIR) spectroscopy. Using DPV to study the interaction of spermidine with DNA, a binding constant (K) value of 4.08 x 10(5)+/-0.05 M(-1) was obtained. For the determination of spermidine, the proposed method exhibited a good dynamic range, correlation coefficient (0.05-1.0 microM and 0.9983, respectively) and a low detection limit (0.02 microM), although Ca(2+) ions were found to electrostatically bind to DNA and weaken the spermidine-DNA interaction. 相似文献
10.