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Chun-he Wan Guang-hua Fu Shao-hua Shi Long-fei Cheng Hong-mei Chen Chun-xiang Peng Su Lin Yu Huang 《中国病毒学》2011,26(5):289-296
Duck circovirus (DuCV), a potential immunosuppressive virus, was investigated in Southern China from March 2006 to December
2009 by using a polymerase chain reaction (PCR) based method. In this study, a total of 138 sick or dead duck samples from
18 different farms were examined with an average DuCV infection rate of ∼35%. It was found that ducks between the ages of
40∼60 days were more susceptible to DuCV. There was no evidence showing that the DuCV virus was capable of vertical transmission.
Farms with positive PCR results exhibited no regularly apparent clinical abnormalities such as feathering disorders, growth
retardation or lower-than-average weight. The complete genomes of 9 strains from Fujian Province and 1 from Zhejiang Province
were sequenced and analyzed. The 10 DuCV genomes, compared with others genomes downloaded from GenBank, ranged in size from
1988 to 1996 base pairs, with sequence identities ranging from 83.2% to 99.8%. Phylogenetic analysis based on genome sequences
demonstrated that DuCVs can be divided into two distinct genetic genotypes, Group I (the Euro-USA lineage) and Group II (the
Taiwan lineage), with approximately 10.0% genetic difference between the two types. Molecular epidemiological data suggest
there is no obvious difference among DuCV strains isolated from different geographic locations or different species, including
Duck, Muscovy duck, Mule duck, Cheery duck, Mulard duck and Pekin duck. 相似文献
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A standardised methodology has been used to define genotypes based on pairwise sequence comparisons (PASC). PASC is a widely accepted method in virus taxonomy, which is based on the histogram of pairwised differences among sequences. Recently, Zhang et al. (2013) concluded that the average p-distance of duck circovirus (DuCV) between genotypes 1 and 2 was 0.170, and subtype distance thresholds were 0.032 in DuCV-1 and 0.018 in DuCV-2, respectively. However, there might be some concerns on the methodology application to define the genotype of DuCV. Taking into account the concerns mentioned above, our authors conducted the PASC analyses of 54 capsid gene (ORF2) and genomic sequences including all the sequences from Zhang et al. (2013). Our results confirmed the existence of two DuCV genotypes (1 and 2) and, we suggest that DuCV ORF2 and genomic distance genotype thresholds were 0.061 and 0.038, respectively. 相似文献
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Zhilong Zhang Renyong Jia Yanyan Lu Mingshu Wang Dekang Zhu Shun Chen Zhongqiong Yin Xiaoyue Chen Anchun Cheng 《Gene》2013
Duck circovirus (DuCV) is a contagious immunosuppressive virus affecting many duck species, which is responsible for multiple outbreaks in poultry industries worldwide. In this study, the first DuCV isolate GH01 was identified in Sichuan by PCR, which shared a high level of nucleotide identity (81.8–99.4%) with sequences of other DuCV isolates available in GenBank. Comparative phylogenetic and pairwise sequence comparison analyses indicated that DuCV could be divided into two genotypes (DuCV-1 and DuCV-2) and six subtypes (1a, 1b, 1c, 2a, 2b and 2c) based on the complete genome sequence. The results revealed that both DuCV-1 and DuCV-2 had evolved from the same ancestor but undergone divergent evolution. Interestingly, phylogenetic analyses indicated that three isolates were classified into a cluster DuCV-2a using complete DuCV genome sequence and cap gene, except rep gene. Recombination analyses revealed that DuCV-2a arose from recombination between DuCV-1a and DuCV-2b isolates within the rep genes, and the recombination events mainly occur both in non-structural protein coding region and structural protein coding region. In addition, the mechanisms of recombination supporting the genetic variability in DuCV isolates were investigated. Likewise, selective pressure indicated that purifying selection had been a major driving force in maintaining diversity among the DuCV isolates. Because eradicating the virus from commercial ducks is impossible, it is necessary to take effective control measures and implement them throughout the world. 相似文献
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Chun-he Wan Guang-hua Fu Shao-hua Shi Long-fei Cheng Hong-mei Chen Chun-xiang Peng Su Lin Yu Huang 《Virologica Sinica》2011,(5)
Duck circovirus (DuCV),a potential immunosuppressive virus,was investigated in Southern China from March 2006 to December 2009 by using a polymerase chain reaction (PCR) based method.In this study,a total of 138 sick or dead duck samples from 18 different farms were examined with an average DuCV infection rate of ~35%.It was found that ducks between the ages of 40~60 days were more susceptible to DuCV.There was no evidence showing that the DuCV virus was capable of vertical transmission.Farms with positive ... 相似文献
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Shi-jin Jiang Xing-xiao Zhang Shao-ning Liu Yu Wang Yi-bo Kong Xiu-li Wei Ya-ni Sun Qin Zhao 《中国病毒学》2008,23(4):265-271
The duck circovirus (DuCV) infection in sick ducks from Fujian Province was investigated. The liver samples of 43 sick Muscovy ducks with infectious serositis were collected from 12 duck farms in Fujian Province.Based on the published sequences of DuCV, two primers were designed for the detection of DuCV and four pairs of primers were designed to amplify four overlapping fragments that cover the complete genome of DuCV. The specific PCR products were amplified from positive samples. The fragments were then cloned into pMD18-T vector and sequenced, and the full length genomic sequence of the FJ0601 isolate of DuCV was obtained. PCR analysis showed that the proportion of ducks which were positive for circovirus was 79% and 10 out of the 12 farms were positive. Sequence analysis showed that the complete genome of DuCV-FJ0601 was 1988 bp and possessed features common to the family Circoviridae which included a stem-loop structure and the Rep protein motifs. Homology analysis showed that FJ0601 isolate of DuCV had 97.3%~97.5% nucleotide sequence identity to all the four Taiwan isolates (TC1/2002, TC2/2002, TC3/2002, TC4/2002), 82.9% identity to the America (33753-52) isolate and 82.3% identity to the Germany isolate. Phylogenetic analysis with Clustal W, however,showed that FJ0601 isolate of DuCV was on a common branch with Taiwan isolates, and Germany and America isolates belonged to the other branch. 相似文献
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