温度及采收期对砀山酥梨耐贮性的影响研究

本文利用灰色系统理论对供试酥梨的适宜贮温、最佳采收期及较优窖形作了综合性研究,结果表明:三种贮温中O℃库温的保鲜效果最理想,关联度达0.9123;就地冷贮以八月底采收为佳,关联度达0.8941;四种供比较的窖形中以3号窖贮藏效果为著,关联度为0.9028．     

湖南通道侗族端午节中的民族植物学

通过长期的民族植物学调查,我们发现通道侗族端午节文化习俗古老,保留有传统的祭祀、药浴、配戴饰品、食用保健野菜等习俗.在端午节中,人们利用的植物种类丰富,用于悬挂门户的植物有4种,涉及4科4属;用于药浴的植物有31种,涉及20科28属;用于保健的野生植物有47种,涉及33科44属.这些植物大多为野生药用植物,它们作用不一,用法也不尽相同,而其中一些能用来悬挂、药浴及保健的植物未见有相关报道,它们所包含的科学知识需要进一步去揭示.通道侗族聚居区的民族植物学知识蕴藏丰富,值得仔细深入地发掘和全面研究.     

Method Optimization for Extracting High-Quality RNA From the Human Pancreas Tissue

Nucleic acid sequencing is frequently used to determine the molecular basis of diseases. Therefore, proper storage of biological specimens is essential to inhibit nucleic acid degradation. RNA isolated from the human pancreas is generally of poor quality because of its high concentration of endogenous RNase. In this study, we optimized the method for extracting high quality RNA from paired tumor and normal pancreatic tissues obtained from eight pancreatic cancer patients post-surgery. RNA integrity number (RIN) was checked to evaluate the integrity of RNA, we tried to extract the RNA with an RIN value of 8 or higher that allows for the latest genetic analysis. The effect of several parameters, including the method used for tissue lysis, RNAlater treatment, tissue weight at storage, and the time to storage after surgical resection, on the quantity and quality of RNA extracted was examined. Data showed that the highest quantity of RNA was isolated using a combination of manual and mechanical methods of tissue lysis. Additionally, sectioning the tissues into small pieces (<100 mg) and treating them with RNAlater solution prior to storage increased RNA stability. Following these guidelines, high quality RNA was obtained from 100% (8/8) of tumor tissues and 75% (6/8) of normal tissues. High-quality RNA was still stable under repeated freezing and thawing. The application of these results during sample handling and storage in clinical settings will facilitate the genetic diagnosis of diseases and their subsequent treatment.     

Quantitative Biomarkers for Prediction of Epidermal Growth Factor Receptor Mutation in Non-Small Cell Lung Cancer

OBJECTIVES: To predict epidermal growth factor receptor (EGFR) mutation status using quantitative radiomic biomarkers and representative clinical variables. METHODS: The study included 180 patients diagnosed as of non-small cell lung cancer (NSCLC) with their pre-therapy computed tomography (CT) scans. Using a radiomic method, 485 features that reflect the heterogeneity and phenotype of tumors were extracted. Afterwards, these radiomic features were used for predicting epidermal growth factor receptor (EGFR) mutation status by a least absolute shrinkage and selection operator (LASSO) based on multivariable logistic regression. As a result, we found that radiomic features have prognostic ability in EGFR mutation status prediction. In addition, we used radiomic nomogram and calibration curve to test the performance of the model. RESULTS: Multivariate analysis revealed that the radiomic features had the potential to build a prediction model for EGFR mutation. The area under the receiver operating characteristic curve (AUC) for the training cohort was 0.8618, and the AUC for the validation cohort was 0.8725, which were superior to prediction model that used clinical variables alone. CONCLUSION: Radiomic features are better predictors of EGFR mutation status than conventional semantic CT image features or clinical variables to help doctors to decide who need EGFR tyrosine kinase inhibitor (TKI) treatment.     

广西三江侗族儿童青少年头面部发育指标分析

本文对广西三江侗族自治县的 7— 16岁乡村侗族男女中、小学生 2 0 87名 (男 10 5 5 ,女 10 32 )的头面部9项指标进行测量 ,并计算有关指数 ,分析和比较了儿童青少年容貌特征发育与年龄增长的关系。根据头面部指数分型 ,确定了广西三江侗族自治县侗族儿童青少年的头面部形态。依各项指数 ,侗族儿童青少年多属圆头型 ,高头型 ,阔头型及阔面型。     

云南新植物

发表天南星属(天南星科)一新种苍山南星Arisaema cangshanense X.D.Dong,通泉草一新变种,白花通泉草Mazus japonica(Thunb.)O, Kuntze var.leucanthus X.D.Dong和光叶蔷薇一新变种,重瓣光叶蔷薇Rosa wichuraiana Crep.var.multipetala X.D.Dong     

黄东海陆架区沉积物中磷的形态分布及生物可利用性

采用1992年Ruttenberg连续提取法(SEDEX)将黄东海陆架区沉积物中的磷分为交换态磷(Ex-P),Fe结合态磷(Fe-P),自生磷(Au-P),碎屑磷(De-P),有机磷(Or-P),分析了各形态磷的平面和垂直分布特征;利用沉积物年代序列测定的结果,探讨了柱状沉积物中不同形态磷的含量变化,并进一步分析了该区域磷形态的生物可利用性.结果表明,黄东海陆架区表层沉积物各形态磷平均含量为:Au-P(140.72 μg/g)＞De-P(59.23 μg/g)＞Or-P(32.69 μg/g)＞Fe-P(29.91 μg/g)＞Ex-P(5.92 μg/g);各形态磷在沉积时间序列上分布不同,反映了不同时期人类活动和气候环境等因子对磷埋藏量影响的不同,其中Au-P在长江口H1-18站位含量比南黄海中部3个站位要低得多;调查区表层潜在生物有效磷为13.55％左右,仅仅占沉积磷中的一小部分.     

侗族新生儿的~Gγ/~Aγ值及一例高~Gγ者的γ基因图谱分析

用聚丙烯酰胺凝胶电泳法测定了广西和湖南省侗族居住区的120例侗族新生儿脐血胎儿血红蛋白中B.subtilis与~Ar比值(~Gr/~Ar值)。显示出119例新生儿的~Gr值(％~Gr)在56—76％之间,均值与标准差为69.4±3.1％;一例新生儿~Gr值高达81.4％,基因图谱分析确定其基因型为~Gr-~(AG)r-~Ar/~Gr-~Ar。     

湘西河流表层沉积物重金属污染特征及其潜在生态毒性风险

花垣河和峒河是湘西地区受到锰矿和铅锌矿生产影响严重的两条河流。通过表层沉积物采样分析了Cd、Pb、Cu、Ni、Cr、Zn和Mn的总量,根据BCR连续提取程序分析沉积物样品中重金属的地球化学赋存形态,采用内梅罗指数法和地积累指数法评价了沉积物重金属污染特征,根据重金属的富集程度探讨了重金属污染来源,采用淡水生态系统沉积物质量基准(SQGs,TEL/PEL)和毒性单位评价了花垣河和峒河沉积物中重金属元素的生态毒性风险。结果表明,花垣河和峒河绝大多数位点的表层沉积物中Cd、Pb、Cu、Ni、Cr、Zn和Mn的总量高于参照点,形成严重的复合污染,花垣河沉积物中重金属的污染水平明显高于峒河,但沿程变化规律不明显,而峒河沉积物中重金属的沿程变化较有规律,即上游含量低,中下游含量较高。两条河流表层沉积物中富集程度居前列的均为Cd、Pb、Zn和Mn。花垣河和峒河沉积物重金属污染主要来源于矿业生产所产生废渣和废水的点排放。在花垣河和峒河的大多数位点,Cd、Pb和Mn的形态具有共同特征,其生物可利用态均较大程度地超过生物不可利用态,而且Mn和Cd的生物可直接利用态所占比例远高于其它重金属,而Cu和Cr的生物可直接利用态所占比例很低。花垣河沉积物中Cd、Pb和Zn在所有位点极大地超过PEL,在峒河中下游,Cd、Pb、Ni和Zn超过PEL,具有较大的潜在生物毒性。除上游S1位点外,花垣河的其余各位点都具有明显的急性毒性,峒河中下游各位点具有明显的急性毒性,这些河段需要重点治理。     

西沙群岛东岛小军舰鸟繁殖种群的初步观察

2003年3-4月对西沙群岛的鸟类资源进行了考察,在西沙群岛的东岛发现小军舰鸟(Fregata minor)繁殖,为迄今所知该种在我国仅有的繁殖记录。野外观察共发现7个巢,其中5个巢中途毁损,另见幼鸟13只,非繁殖雄性个体2只。还观察到小军舰鸟与红脚鲣鸟相互影响的负面行为,如抢食、窃巢材等。调查结果显示,西沙群岛小军舰鸟分布范围现已缩小,而且种群数量显著下降。