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Molecular Cloning and Regional Distribution of a Human Proton Receptor
Subunit with Biphasic Functional Properties 总被引:8,自引:0,他引:8
Abstract : Small changes of extracellular pH activate depolarizing inward currents in most nociceptive neurons. It has been recently proposed that acid sensitivity of sensory as well as central neurons is mediated by a family of proton-gated cation channels structurally related to Caenorhabditis elegans degenerins and mammalian epithelial sodium channels. We describe here the molecular cloning of a novel human proton receptor, hASIC3, a 531-amino acid-long subunit homologous to rat DRASIC. Expression of homomeric hASIC3 channels in Xenopus oocytes generated biphasic inward currents elicited at pH <5, providing the first functional evidence of a human proton-gated ion channel. Contrary to the DRASIC current phenotype, the fast desensitizing early component and the slow sustained late component differed both by their cationic selectivity and by their response to the antagonist amiloride, but not by their pH sensitivity (pH50 = 3.66 vs. 3.82). Using RT-PCR and mRNA blot hybridization, we detected hASIC3 mRNA in sensory ganglia, brain, and many internal tissues including lung and testis, so hASIC3 gene expression was not restricted to peripheral sensory neurons. These functional and anatomical data strongly suggest that hASIC3 plays a major role in persistent proton-induced currents occurring in physiological and pathological conditions of pH changes, likely through a tissue-specific heteropolymerization with other members of the proton-gated channel family. 相似文献
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Nina Boiko Volodymyr Kucher James D. Stockand Benjamin A. Eaton 《The Journal of biological chemistry》2012,287(47):39878-39886
The molecular transformation of an external stimulus into changes in sensory neuron activity is incompletely described. Although a number of molecules have been identified that can respond to stimuli, evidence that these molecules can transduce stimulation into useful neural activity is lacking. Here we demonstrate that pickpocket1 (ppk1), a Drosophila homolog of mammalian Degenerin/epithelial sodium channels, encodes an acid-sensing sodium channel that conducts a transient depolarizing current in multidendritic sensory neurons of Drosophila melanogaster. Stimulation of Ppk1 is sufficient to bring these sensory neurons to threshold, eliciting a burst of action potentials. The transient nature of the neural activity produced by Ppk1 activation is the result of Ppk1 channel gating properties. This model is supported by the observation of enhanced bursting activity in neurons expressing a gain of function ppk1 mutant harboring the degenerin mutation. These findings demonstrate that Ppk1 can function as an ionotropic molecular sensory transducer capable of transforming the perception of a stimulus into phasic neuronal activity in sensory neurons. 相似文献
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One of the looming mysteries in signal transduction today is the question of how mechanical signals, such as pressure or mechanical
force delivered to a cell, are interpreted to direct biological responses. All living organisms, and probably all cells, have
the ability to sense and respond to mechanical stimuli. At the single-cell level, mechanical signaling underlies cell-volume
control and specialized responses such as the prevention of poly-spermy in fertilization. At the level of the whole organism,
mechanotransduction underlies processes as diverse as stretch-activated reflexes in vascular epithelium and smooth muscle;
gravitaxis and turgor control in plants; tissue development and morphogenesis; and the senses of touch, hearing, and balance.
Intense genetic, molecular, and elecrophysiological studies in organisms ranging from nematodes to mammals have highlighted
members of the recently discovered DEG/ENaC family of ion channels as strong candidates for the elusive metazoan mechanotransducer.
Here, we discuss the evidence that links DEG/ENaC ion channels to mechanotransduction and review the function of Caenorhabiditis elegans members of this family called degenerins and their role in mediating mechanosensitive behaviors in the worm. 相似文献
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