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1.
Additive hazards regression for case-cohort studies 总被引:3,自引:0,他引:3
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Microorganisms surviving for 5300 years 总被引:2,自引:0,他引:2
Abstract Recently, the well-preserved corpse of a prehistoric man with an age of approximately 5300 years bp was discovered in the Central European Alps. Analysis of materials associated with the individual has revealed the presence of microorganisms which are believed to have survived since the time of death. So far, two fungi have been isolated and identified as species of the genera Chaetomium and Absidia , respectively. In addition, we have obtained one bacterial isolate which we have identified as a Streptomyces species. Our findings demonstrate that microorganisms can remain viable under appropriate circumstances for thousands of years. The isolates may enable us to study evolutionary trends within microorganisms. 相似文献
4.
The Yanomama Indians of Southern Vanezuela and Northern Brazil are one of the largest, relatively unacculturated tribes of the tropical rain forest. Over a period of eight years data have been collected from a considerable portion of their territory on estimated age, sex ratio, fertility rates (as determined by physical examination and urine tests), and infant death rates. Although it has been impossible to collect direct data on infanticide, this subject can be approached indirectly through distortions of the sex ratio and anecdotal information. Some historical data are also available as a basis for estimating tribal expansion in the past 100 years. With this material it has been possible to construct Life Tables for the Yanomama, and to explore the results of various perturbations of the input parameters. Data are also presented on patterns of mating and reproduction: number of spouses, mean and variance in number of surviving children, frequency of “extra-marital conceptions” based on the results of extensive blood group typings, and consanguinity rates as determined by observation and computer simulation. Although we do not present the Yanomama as typical, these data are seen as providing a basis for more realistic population models than have existed in the past. In addition, the data provide a basis for relatively precise estimates of such demographic measures as Fisher's Reproductive Value, Crow's Index of Total Selection, and Weiss' Index of Growth Regulation. 相似文献
5.
J. H. Becking 《Plant and Soil》1987,100(1-3):183-212
Summary The survival of Azolla was studied in an artificial system which simulated the soil/water interface and the desiccation of
soil during a fallow period in lowland rice culture. Tests with non-sporulating and sporulating Azolla fronds showed that
Azolla only survives with sporulated fronds. At their reappearance the Azolla fronds already harboured the Anabaena endophyte.
A detailed light microscopic and transmission electron microscopic study of macro- and micros-porocarp formation and development
revealed that the endophyte is transmitted by the macrosporocarps and not by the microsporocarps. The Anabaena cells within
the macrosporocarps are found just below the indusium cap. These cells are not nitrogen-fixing akinetes. The free-living Anabaena
cells at the stem apex and below the overarching developing leaves do not bear heterocysts and accordingly are non nitrogen-fixing.
During the development of the leaf the Anabaena enters the leaf cavity, but later the pore of this, cavity closes and the
imprisoned cyanobacteria are lysed before the leaf decays. As the Azolla leaves age a nitrogen-fixing capability is successively
built up concomittantly with the production of heterocysts. Heterocyst frequencies of 40–50% can be found inAnabaena azollae. Usually a gradient of nitrogen-fixing capacity occurs along the Azolla rhizome with two distinct peaks at leaf number 7/8
and at leaf number 13/14 from the apex. 相似文献
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8.
鳗鲡幼鱼耳石日轮的研究 总被引:16,自引:1,他引:15
本文报道采自辽东半岛沿岸鳗鲡(Anguilla japonica)的白仔鳗和经人工培育的当年幼鳗耳石日轮生长的观察结果。白仔鳗和幼鳗耳石平均直径均与体全长成直线相关。12尾白仔鳗耳石的平均日轮数146.3,据此推测其产卵期为11—12月。观察证实从咸淡水转人到淡水生活的幼鳗耳石的环纹有过渡带存在。 相似文献
9.
Long-term survival of Legionella pneumophila serogroup 1 under low-nutrient conditions and associated morphological changes 总被引:1,自引:0,他引:1
Christine Paszko-Kolva Manouchehr Shahamat Rita R. Colwell 《FEMS microbiology letters》1992,102(1):45-55
Abstract Extended survival of Legionella pneumophila , using both a clinical and an environmental isolate, was studied in drinking water, creek water, and estuarine water microcosms. Legionella populations were monitored by acridine orange direct counts (AODC) and viable count on buffered charcoal yeast extract agar amended with alpha-ketoglutarate (BCYEα). Initial colony counts of the clinical isolate in drinking and creek water microcosms were 2 × 108 cfu/ml and, after incubation for 1.5 years, the plate counts decreased to 3 × 106 cfu/ml. The AODC counts, however, did not change significantly. The clinical isolate in estuarine water decreased in plate counts to 102 (cfu/ml) over the same period. After incubation for 1.5 years at 15°C in the microcosms, Legionella plate counts of creek and drinking water decreased by two logs. Direct microscopic examination of aliquots removed from all microcosms revealed the presence of small bacilli, large bacilli and rare filamentous cells. The environmental isolate demonstrated only one colony morphology upon culture on BCYEα. Interestingly, after four months incubation in the microcosm, upon plating the clinical isolate on BCYEα, two distinct colony types were evident. Examination by immunofluorescent staining employing a monoclonal antibody against L. pneumophila revealed both bacillus and filamentous forms. The total cellular proteins of both morphotypes were examined by sodium dodecyl sulfate polyacrylyamide gel electrophoresis (SDS-PAGE), demonstrating identical protein patterns. Those Legionella cells remaining culturable during 1.5 years of incubation grew rapidly when transferred to BCYEα. Incubation was continued and it was found that some strains of L. pneumophila serogroup 1 can remain viable for longer than 2.4 years under low-nutrient conditions. 相似文献
10.
Roy E. Crabtree Edward C. Cyr Renée E. Bishop Laura M Falkenstein John M. Dean 《Environmental Biology of Fishes》1992,35(4):361-370
Synopsis Leptocephali were collected in June 1981 and July 1989 over the continental shelf and slope of the Florida west coast. Tarpon larvae ranged 5.5–24.4 mm standard length (SL) and were the second most abundant leptocephalus species. Sagittae examined with compound microscopes and scanning electron microscopy had increments that were presumed to be formed daily. Increment counts made using the two microscopic techniques were not significantly different. Estimated ages ranged 2–25 days with a growth rate (± standard error) of 0.92 ± 0.04 mm d–1 The least squares linear regression equation SL = 2.78 + 0.92 (age in days) best described the relationship between estimated age and length. Adult tarpon appear to undergo a substantial spawning migration from inshore areas frequented during spring and summer to offshore spawning grounds. Spawning occurs during May, June, and July, although the spawning season may be of greater duration. 相似文献